Preparation and Characterization of Attractive Poly(amino acid) Hydrogels Based on 2-Ureido-4[1<italic style="font-style: italic">H</italic>]-pyrimidinone

Zhen Shi; Qi Wang; Gui-Fei Li; Yu-Feng Shou; Hong-Jie Zong; Shi-Feng Yan; Kun-Xi Zhang; Jing-Bo Yin

doi:10.1007/s10118-021-2498-y

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Preparation and Characterization of Attractive Poly(amino acid) Hydrogels Based on 2-Ureido-4[1H]-pyrimidinone

ARTICLE | Updated：2020-10-15

- Preparation and Characterization of Attractive Poly(amino acid) Hydrogels Based on 2-Ureido-4[1H]-pyrimidinone
- Chinese Journal of Polymer Science Vol. 39, Issue 3, Pages: 327-336(2021)
- Affiliations：
  
  1.Department of Polymer Materials, School of Materials Science and Engineering, Shanghai University, Shanghai 200444, China
- Author bio：
  
  guifeili@shu.edu.cn (G.F.L.)
  jbyin@oa.shu.edu.cn (J.B.Y.)
- Funds：
- DOI：10.1007/s10118-021-2498-y
  CLC：
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Zhen Shi, Qi Wang, Gui-Fei Li, et al. Preparation and Characterization of Attractive Poly(amino acid) Hydrogels Based on 2-Ureido-4[1H]-pyrimidinone. [J]. Chinese Journal of Polymer Science 39(3):327-336(2021)
DOI：

Zhen Shi, Qi Wang, Gui-Fei Li, et al. Preparation and Characterization of Attractive Poly(amino acid) Hydrogels Based on 2-Ureido-4[1H]-pyrimidinone. [J]. Chinese Journal of Polymer Science 39(3):327-336(2021) DOI： 10.1007/s10118-021-2498-y.

摘要

Abstract

Self-healing hydrogels with the shear-thinning property are novel injectable materials and are superior to traditional injectable hydrogels. The self-healing hydrogels based on 2-ureido-4[1,H,]-pyrimidinone (UPy) have recently received extensive attention due to their dynamic reversibility of UPy dimerization. However, generally, UPy-based self-healing hydrogels exhibit poor stability, cannot degrade ,in vivo, and can hardly be excreted from the body, which considerably limit their bio-application. Here, using poly(,l,-glutamic acid) (PLGA) as biodegradable matrix, branching ,α,-hydroxy-,ω,-amino poly(ethylene oxide) (HAPEO) as bridging molecule to introduce UPy, and ethyl acrylate polyethylene glycol (MAPEG) to introduce double bond, the hydrogel precursors (PMHU) are prepared. A library of the self-healing hydrogels has been achieved with well self-healable and shear-thinning properties. With the increase of MAPEG grafting ratio, the storage modulus of the self-healing hydrogels decreases. The self-healing hydrogels are stable in solution only for 6 h, hard to meet the requirements of tissue regeneration. Consequently, ultraviolet (UV) photo-crosslinking is involved to obtain the dual crosslinking hydrogels with enhanced mechanical properties and stability. When MAPEG grafting ratio is 35.5%, the dual crosslinking hydrogels can maintain the shape in phosphate-buffered saline solution (PBS) for at least 8 days. Loading with adipose-derived stem cell spheroids, the self-healing hydrogels are injected and self-heal to a whole, and then they are crosslinked ,in situ via, UV-irradiation, obtaining the dual crosslinking hydrogels/cell spheroids complex with cell viability of 86.7%±6.0%, which demonstrates excellent injectability, subcutaneous gelatinization, and biocompatibility of hydrogels as cell carriers. The novel PMHU hydrogels crosslinked by quadruple hydrogen bonding and then dual photo-crosslinking of double bond are expected to be applied for minimal invasive surgery or therapies in tissue engineering.

关键词

Keywords

2-Ureido-4[1H]-pyrimidinone (UPy)Poly(l-glutamic acid) (PLGA)Self-healablePhoto-crosslinking

references

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Department of Chemical and Biomolecular Engineering, National University of Singapore

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Preparation and Characterization of Attractive Poly(amino acid) Hydrogels Based on 2-Ureido-4[1H]-pyrimidinone

DOI：10.1007/s10118-021-2498-y

摘要

Abstract

关键词

Keywords

references