Preparation and Characterization of Attractive Poly(amino acid) Hydrogels Based on 2-Ureido-4[1<italic style="font-style: italic">H</italic>]-pyrimidinone

Zhen Shi; Qi Wang; Gui-Fei Li; Yu-Feng Shou; Hong-Jie Zong; Shi-Feng Yan; Kun-Xi Zhang; Jing-Bo Yin

doi:10.1007/s10118-021-2498-y

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Preparation and Characterization of Attractive Poly(amino acid) Hydrogels Based on 2-Ureido-4[1H]-pyrimidinone

ARTICLE | Updated：2020-10-15

- Preparation and Characterization of Attractive Poly(amino acid) Hydrogels Based on 2-Ureido-4[1H]-pyrimidinone
- Chinese Journal of Polymer Science Vol. 39, Issue 3, Pages: 327-336(2021)
- Affiliations：
  
  Department of Polymer Materials, School of Materials Science and Engineering, Shanghai University, Shanghai 200444, China
- Author bio：
  
  guifeili@shu.edu.cn (G.F.L.)
  jbyin@oa.shu.edu.cn (J.B.Y.)
- Funds：
- DOI：10.1007/s10118-021-2498-y
  CLC：
- Received：14 July 2020，
  
  Revised：2020-8-28，
  
  Accepted：31 August 2020，
  
  Online First：15 October 2020，
  
  Published：02 January 2021
- Accepted：
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Shi, Z.; Wang, Q.; Li, G. F.; Shou, Y. F.; Zong, H. J.; Yan, S. F.; Zhang, K. X.; Yin, J. B. Preparation and characterization of attractive poly(amino acid) hydrogels based on 2-ureido-4[1, H,]-pyrimidinone. Chinese J. Polym. Sci. 2021, 39, 327–336

Zhen Shi, Qi Wang, Gui-Fei Li, et al. Preparation and Characterization of Attractive Poly(amino acid) Hydrogels Based on 2-Ureido-4[1H]-pyrimidinone[J]. Chinese Journal of Polymer Science, 2021, 39(3): 327-336. DOI: 10.1007/s10118-021-2498-y.
Shi, Z.; Wang, Q.; Li, G. F.; Shou, Y. F.; Zong, H. J.; Yan, S. F.; Zhang, K. X.; Yin, J. B. Preparation and characterization of attractive poly(amino acid) hydrogels based on 2-ureido-4[1, H,]-pyrimidinone. Chinese J. Polym. Sci. 2021, 39, 327–336 DOI： 10.1007/s10118-021-2498-y.

Zhen Shi, Qi Wang, Gui-Fei Li, et al. Preparation and Characterization of Attractive Poly(amino acid) Hydrogels Based on 2-Ureido-4[1H]-pyrimidinone[J]. Chinese Journal of Polymer Science, 2021, 39(3): 327-336. DOI: 10.1007/s10118-021-2498-y. DOI：

摘要

Abstract

Self-healing hydrogels with the shear-thinning property are novel injectable materials and are superior to traditional injectable hydrogels. The self-healing hydrogels based on 2-ureido-4[1

]-pyrimidinone (UPy) have recently received extensive attention due to their dynamic reversibility of UPy dimerization. However

generally

UPy-based self-healing hydrogels exhibit poor stability

cannot degrade

in vivo

and can hardly be excreted from the body

which considerably limit their bio-application. Here

using poly(

-glutamic acid) (PLGA) as biodegradable matrix

branching

-hydroxy-

-amino poly(ethylene oxide) (HAPEO) as bridging molecule to introduce UPy

and ethyl acrylate polyethylene glycol (MAPEG) to introduce double bond

the hydrogel precursors (PMHU) are prepared. A library of the self-healing hydrogels has been achieved with well self-healable and shear-thinning properties. With the increase of MAPEG grafting ratio

the storage modulus of the self-healing hydrogels decreases. The self-healing hydrogels are stable in solution only for 6 h

hard to meet the requirements of tissue regeneration. Consequently

ultraviolet (UV) photo-crosslinking is involved to obtain the dual crosslinking hydrogels with enhanced mechanical properties and stability. When MAPEG grafting ratio is 35.5%

the dual crosslinking hydrogels can maintain the shape in phosphate-buffered saline solution (PBS) for at least 8 days. Loading with adipose-derived stem cell spheroids

the self-healing hydrogels are injected and self-heal to a whole

and then they are crosslinked

in situ via

UV-irradiation

obtaining the dual crosslinking hydrogels/cell spheroids complex with cell viability of 86.7%±6.0%

which demonstrates excellent injectability

subcutaneous gelatinization

and biocompatibility of hydrogels as cell carriers. The novel PMHU hydrogels crosslinked by quadruple hydrogen bonding and then dual photo-crosslinking of double bond are expected to be applied for minimal invasive surgery or therapies in tissue engineering.

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Preparation and Characterization of Attractive Poly(amino acid) Hydrogels Based on 2-Ureido-4[1H]-pyrimidinone

DOI：10.1007/s10118-021-2498-y

摘要

Abstract

关键词

Keywords

references