NIR Emission Nanoparticles Based on FRET Composed of AIE Luminogens and NIR Dyes for Two-photon Fluorescence Imaging

Lei-Jing Liu; Wen Liu; Guang Ji; Zhi-Yuan Wu; Bin Xu; Jun Qian; Wen-Jing Tian

doi:10.1007/s10118-019-2206-3

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NIR Emission Nanoparticles Based on FRET Composed of AIE Luminogens and NIR Dyes for Two-photon Fluorescence Imaging

ARTICLE | Updated：2021-02-18

- NIR Emission Nanoparticles Based on FRET Composed of AIE Luminogens and NIR Dyes for Two-photon Fluorescence Imaging
- Chinese Journal of Polymer Science Vol. 37, Issue 4, Pages: 401-408(2019)
- Affiliations：
  
  a.State Key Lab for Supramolecular Structure and Materials, Jilin University, Changchun 130012, China
  b.State Key Laboratory of Modern Optical Instrumentations, Centre for Optical and Electromagnetic Research, JORCEP (Sino-Swedish Joint Research Center of Photonics), Zhejiang University, Hangzhou 310058, China
- Author bio：
  
  qianjun@zju.edu.cn (J.Q.)
  wjtian@jlu.edu.cn (W.J.T.)
- Funds：
- DOI：10.1007/s10118-019-2206-3
  CLC：
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Lei-Jing Liu, Wen Liu, Guang Ji, et al. NIR Emission Nanoparticles Based on FRET Composed of AIE Luminogens and NIR Dyes for Two-photon Fluorescence Imaging. [J]. Chinese Journal of Polymer Science 37(4):401-408(2019)
DOI：

Lei-Jing Liu, Wen Liu, Guang Ji, et al. NIR Emission Nanoparticles Based on FRET Composed of AIE Luminogens and NIR Dyes for Two-photon Fluorescence Imaging. [J]. Chinese Journal of Polymer Science 37(4):401-408(2019) DOI： 10.1007/s10118-019-2206-3.

摘要

Abstract

Near-infrared (NIR) nanoparticles (NPs) based on fluorescence resonance energy transfer (FRET) were prepared by co-encapsulation of a red aggregation-induced emission (AIE) molecule, 2-(4-bromophenyl)-3-(4-(4-(diphenylamino)styryl)phenyl)fumaronitrile (TB), and a commercial NIR fluorescence dye, silicon 2,3-naphthalocyanine bis(trihexylsilyloxide) (NIR775) with an amphiphilic polymer poly(styrene-,co,-maleic anhydride) (PSMA). The surface of the NPs, PSMA@TB/NIR775, was modified with poly(ethylene glycol) (PEG) to increase the ,in vivo, biocompatibility of the NPs. The PSMA@TB/NIR775 NPs showed a strong NIR (780 nm) narrow emission and excellent two-photon absorption property. Moreover, the NPs exhibited good monodispersity, stability, and low cytotoxicity. Under the excitation of a 1040 nm femtosecond (fs) laser, the emission peaks at 680 nm of TB and 780 nm of NIR775 excited by FRET were obtained. We utilized PSMA@TB/NIR775 NPs as fluorescent contrast agents for two-photon excited NIR microscopic imaging, and good NIR imaging effect of mouse brain vasculature was obtained with the imaging depth of about 150 µm. The FRET strategy by co-encapsulating AIE molecule and NIR dye will be helpful in preparing more narrow emission NIR probes for deep-tissue biological imaging.

关键词

Keywords

NIR emissionAIEFRETTwo-photon imagingAmphiphilic polymer

references

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