To explore construction of novel mimicking biomembrane on biomaterials surfaces, a new polymerizable phosphatidylcholine containing a long monoalkyl chain ended with acryl group (AASOPC) was designed and synthesized, which was easily derived from the terminal amino group of 9-(2-amino-ethylcarbamoyl)-nonyl-1-phosphatidyl-choline (ASOPC) reacting with acryloyl chloride. The obtained AASOPC was grafted on poly(ethylene terephthalate) (PET) ,via, surface-initiated atom-transfer radical polymerization (SI-ATRP) to form mimicking biomembrane. These modified surface structures of PET were investigated using water contact angle (WAC), X-ray photoelectron spectroscopy (XPS) and atomic force microscopy (AFM). The results indicated that the new mimicking phosphatidylcholine biomembrane could be prepared on inert polymer surfaces by using the acryloyl phosphatidylcholine (AASOPC) ,via, surface-initiated atom transfer radical polymerization (SI-ATRP).