Amine-reactive Polymer Platform for Engineering Surface Modification of Next-generation Sequencing Chips

Wei Tian; Xin-Yuan Wang; Die-Wen Feng; Xiang-Qian Li; Yue-Kang Jin; Hui Li; Hao Liu

doi:10.1007/s10118-025-3413-8

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Amine-reactive Polymer Platform for Engineering Surface Modification of Next-generation Sequencing Chips

RESEARCH ARTICLE | Updated：2025-10-30

- Amine-reactive Polymer Platform for Engineering Surface Modification of Next-generation Sequencing Chips
- Amine-reactive Polymer Platform for Engineering Surface Modification of Next-generation Sequencing Chips
- 高分子科学（英文） 2025年43卷第11期页码：2030-2041
- Affiliations：
  
  a.State Key Laboratory of Advanced Fiber Materials, Center for Advanced Low-Dimension Materials, Donghua University, Shanghai 201620, China
  b.Salus BioMed Company, Shenzhen 518106, China
  c.Institute of Advanced Technology, University of Science and Technology of China, Hefei 230026, China
- Author bio：
  
  jinyk@mail.ustc.edu.cn (Y.K.J.)
  lihui@dhu.edu.cn (H.L.)
  liuh@dhu.edu.cn (H.L.)
- Funds：
  
  This work was financially supported by the Science and Technology Commission of Shanghai Municipality (No. 24ZR1401400). The authors would like to express their sincere gratitude to Shenzhen Salus BioMed Company for their strong support in this study. We are grateful for the generosity of the company in providing the sequencing facilities and reagents.;Electronic supplementary information (ESI) is available free of charge in the online version of this article at http://doi.org/10.1007/s10118-025-3413-8.
- DOI：10.1007/s10118-025-3413-8
  中图分类号：
- 收稿：2025-06-10，
  
  修回：2025-07-09，
  
  录用：2025-07-09，
  
  网络首发：2025-09-02，
  
  纸质出版：2025-11-05
- Accepted：
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Tian, W.; Wang, X. Y.; Feng, D. W.; Li, X. Q.; Jin, Y. K.; Li, H.; Liu, H. Amine-reactive polymer platform for engineering surface modification of next-generation sequencing chips. Chinese J. Polym. Sci. 2025, 43, 2030–2041

Wei Tian, Xin-Yuan Wang, Die-Wen Feng, et al. Amine-reactive Polymer Platform for Engineering Surface Modification of Next-generation Sequencing Chips[J]. Chinese Journal of Polymer Science, 2025, 43(11): 2030-2041.
Tian, W.; Wang, X. Y.; Feng, D. W.; Li, X. Q.; Jin, Y. K.; Li, H.; Liu, H. Amine-reactive polymer platform for engineering surface modification of next-generation sequencing chips. Chinese J. Polym. Sci. 2025, 43, 2030–2041 DOI： 10.1007/s10118-025-3413-8.

Wei Tian, Xin-Yuan Wang, Die-Wen Feng, et al. Amine-reactive Polymer Platform for Engineering Surface Modification of Next-generation Sequencing Chips[J]. Chinese Journal of Polymer Science, 2025, 43(11): 2030-2041. DOI： 10.1007/s10118-025-3413-8.

摘要

Part (a) illustrates the post-polymerization modification of the PPFPA platform with Monomer-1 and Monomer-2

introducing azide groups and hydrophilic functionalities. Part (b) shows the covalent anchoring of the surface modification polymer onto the sequencing chip surface via click chemistry.

Abstract

In this study

an amine-reactive poly(pentafluorophenyl acrylate) (

PPFPA

) platform was developed for advanced surface engineering of next-generation sequencing (NGS) chips. Through post-polymerization modification

PPFPA

was functionalized with dual moieties: azide groups for covalent immobilization of DBCO-modified DNA primers

via

click chemistry and tunable hydrophilic side chains to optimize biocompatibility and surface properties. Systematic screening revealed that hydrophobic azide carriers combined with neutral hydroxyl groups maximized the DNA immobilization efficacy

approaching the performance of commercial polyacrylamide-based polymers. The negatively charged carboxyl groups severely impede DNA primer attachment. Higher molecular weight derivatives further enhance the efficacy of DNA immobilization. In NGS validation

optimized surface modification polymers achieved robust surface density of clustered DNA and high sequencing accuracy

surpassing quality benchmarks and comparable to those of conventional analogs. This platform demonstrates significant potential for tailoring high-sensitivity surfaces for genomic applications

advancing clinical diagnostics

and personalized medicine.

关键词

Keywords

references

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