Production of Functional Materials Derived from Regenerated Silk Fibroin by Utilizing 3D Printing and Biomimetic Enzyme-induced Mineralization

Ni Chen; Fei-Yu Luo; Gong-Wen Yang; Jin-Rong Yao; Xin Chen; Zheng-Zhong Shao

doi:10.1007/s10118-023-3059-3

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Production of Functional Materials Derived from Regenerated Silk Fibroin by Utilizing 3D Printing and Biomimetic Enzyme-induced Mineralization

RESEARCH ARTICLE | Updated：2024-02-20

- Production of Functional Materials Derived from Regenerated Silk Fibroin by Utilizing 3D Printing and Biomimetic Enzyme-induced Mineralization
- Production of Functional Materials Derived from Regenerated Silk Fibroin by Utilizing 3D Printing and Biomimetic Enzyme-induced Mineralization
- 高分子科学（英文版） 2024年42卷第3期页码：299-310
- Affiliations：
  
  State Key Laboratory of Molecular Engineering of Polymers, Laboratory of Advanced Materials and Department of Macromolecular Science, Fudan University, Shanghai 200433, China
- Author bio：
  
  zzshao@fudan.edu.cn
- Funds：
  
  This work was financially supported by the National Nature Science Foundation of China (No. 21935002). We also sincerely thank Gao Han (Ph.D candidate) at Fudan University for his support in cell experiment.;Electronic supplementary information (ESI) is available free of charge in the online version of this article at http://doi.org/10.1007/s10118-023-3059-3.
- DOI：10.1007/s10118-023-3059-3
  中图分类号：
- 收稿：2023-08-23，
  
  修回：2023-10-11，
  
  录用：2023-10-12，
  
  网络首发：2023-11-15，
  
  纸质出版：2024-03-01
- Accepted：
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Chen, N.; Luo, F. Y.; Yang, G. W.; Yao, J. R.; Chen, X.; Shao, Z. Z. Production of functional materials derived from regenerated silk fibroin by utilizing 3D printing and biomimetic enzyme-induced mineralization. Chinese J. Polym. Sci. 2024, 42, 299–310

Ni Chen, Fei-Yu Luo, Gong-Wen Yang, et al. Production of Functional Materials Derived from Regenerated Silk Fibroin by Utilizing 3D Printing and Biomimetic Enzyme-induced Mineralization[J]. Chinese Journal of Polymer Science, 2024, 42(3): 299-310.
Chen, N.; Luo, F. Y.; Yang, G. W.; Yao, J. R.; Chen, X.; Shao, Z. Z. Production of functional materials derived from regenerated silk fibroin by utilizing 3D printing and biomimetic enzyme-induced mineralization. Chinese J. Polym. Sci. 2024, 42, 299–310 DOI： 10.1007/s10118-023-3059-3.

Ni Chen, Fei-Yu Luo, Gong-Wen Yang, et al. Production of Functional Materials Derived from Regenerated Silk Fibroin by Utilizing 3D Printing and Biomimetic Enzyme-induced Mineralization[J]. Chinese Journal of Polymer Science, 2024, 42(3): 299-310. DOI： 10.1007/s10118-023-3059-3.

摘要

The 3D-printed RSF-hydroxyapatite composite materials that were prepared through a two-step technique by integrating 3D printing technology and ALP-induced biomimetic mineralization

demonstrated a promising bone substitute material with intricate structure

adjustable compression modulus of megapascal grade and variable hydroxyapatite content.

Abstract

Critical-sized bone defects

commonly encountered in clinical orthopedic surgery

present a significant challenge. One of the promising solutions is to prepare synthetic bone substitute materials with precise structural control

mechanical compatibility

and enhanced osteogenic induction performance

nevertheless the successful preparation of such materials remains difficult. In this study

a two-step technique

integrating an extrusion-based printing process with biomimetic mineralization induced by alkaline phosphatase (ALP)

was developed. Initially

a pre-cured hydrogel of regenerated silk fibroin (RSF) with a small quantity of hydroxypropyl cellulose (HPC) and ALP was prepared through heating the mixed aqueous solution. This pre-cured hydrogel demonstrated thixotropic property and could be directly extruded into predetermined structures through a 3D-printer. Subsequently

the 3D-printed RSF-based materials with ALP underwent biomimetic

in situ

mineralization in calcium glycerophosphate (Ca-GP) mineralizing solution

utilizing the polymer chains of RSF as templates and ALP as a trigger for cleaving phosphate bonds of Ca-GP. The resulting 3D-printed RSF-mineral composites including hydrogel and sponge possessed adjustable compression modulus of megapascal grade and variable hydroxyapatite content

which could be controlled by manipulating the duration of the mineralization process. Moreover

these 3D-printed RSF-mineral composites demonstrated non-cytotoxicity towards rat bone marrow mesenchymal stem cells. Therefore

they may hold great potential for applications involving the replacement of tissues characterized by osteoinductivity and intricate structures.

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