Molecular Chaperone-Dependent Polymer Translocation through Nanopores: The Effects of Chaperone Concentration and Chaperone-Polymer Interaction

Chang-Sheng Zuo; Kang Wang; Li-Zhen Sun; Ting-Ting Sun

doi:10.1007/s10118-023-3016-1

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Molecular Chaperone-Dependent Polymer Translocation through Nanopores: The Effects of Chaperone Concentration and Chaperone-Polymer Interaction

RESEARCH ARTICLE | Updated：2023-12-26

- Molecular Chaperone-Dependent Polymer Translocation through Nanopores: The Effects of Chaperone Concentration and Chaperone-Polymer Interaction
- Molecular Chaperone-Dependent Polymer Translocation through Nanopores: The Effects of Chaperone Concentration and Chaperone-Polymer Interaction
- 高分子科学（英文版） 2024年42卷第1期页码：125-132
- Affiliations：
  
  a.Department of Physics, Zhejiang University of Science and Technology, Hangzhou 310023, China
  b.Department of Applied Physics, Zhejiang University of Technology, Hangzhou 310014, China
- Author bio：
  
  sunlizhen@zjut.edu.cn (L.Z.S.)
  tingtingsun@zust.edu.cn (T.T.S.)
- Funds：
  
  This work was financially supported by the National Natural Science Foundation of China (Nos. 11704333 and 20904047) and the Natural Science Foundation of Zhejiang Province (Nos. LY17A040001 and LY19F030004).
- DOI：10.1007/s10118-023-3016-1
  中图分类号：
- 收稿：2023-05-08，
  
  修回：2023-6-1，
  
  录用：2023-6-4，
  
  网络首发：2023-07-24，
  
  纸质出版：2024-01-01
- Accepted：
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Zuo, C. S.; Wang, K.; Sun, L. Z.; Sun, T. T. Molecular chaperone-dependent polymer translocation through nanopores: the effects of chaperone concentration and chaperone-polymer interaction. Chinese J. Polym. Sci. 2024, 42, 125–132

Chang-Sheng Zuo, Kang Wang, Li-Zhen Sun, et al. Molecular Chaperone-Dependent Polymer Translocation through Nanopores: The Effects of Chaperone Concentration and Chaperone-Polymer Interaction[J]. Chinese Journal of Polymer Science, 2024, 42(1): 125-132.
Zuo, C. S.; Wang, K.; Sun, L. Z.; Sun, T. T. Molecular chaperone-dependent polymer translocation through nanopores: the effects of chaperone concentration and chaperone-polymer interaction. Chinese J. Polym. Sci. 2024, 42, 125–132 DOI： 10.1007/s10118-023-3016-1.

Chang-Sheng Zuo, Kang Wang, Li-Zhen Sun, et al. Molecular Chaperone-Dependent Polymer Translocation through Nanopores: The Effects of Chaperone Concentration and Chaperone-Polymer Interaction[J]. Chinese Journal of Polymer Science, 2024, 42(1): 125-132. DOI： 10.1007/s10118-023-3016-1.

摘要

Chaperone-accompanied polymer translocation through an nanopore are theoretical studied by calculating free energy landscape and translocation time. The translocation dynamics can be controlled by tuning chaperone concentration and chaperone-polymer interaction. The chaperone-assisted

chaperone-independent

and chaperone-hindered translocation events are observed at different chaperone conditions.

Abstract

The polymer translocation through a nanopore from a donor space (or named

cis

side) to a receiver space (

trans

side) in the chaperone-induced crowded environment has attracted increasing attention in recent years due to its significance in biological systems and technological applications. In this work

we mainly focus on the effects of chaperone concentration and chaperone-polymer interaction on the polymer translocation. By assuming the polymer translocation to be a quasi-equilibrium process

the free energy

of the polymer can be estimated by Rosenbluth-Rosenbluth method and then the translocation time

can be calculated by Fokker-Plank equation based on the obtained free energy landscape. Our calculation results show that the translocation time can be controlled by independently tuning the chaperone concentration and chaperone-polymer interaction at the

cis

side or the

trans

side. There exists a critical chaperone-polymer attraction

=−0.2 at which the volume exclusion and interaction effects of the chaperone can balance each other. Additionally

we also find that at large chaperone-polymer attraction

the translocation time is mainly governed by the diffusion coefficient of the polymer.

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references

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