Engineering Antibacterial Activities and Biocompatibility of Hyperbranched Lysine-based Random Copolymers

Hui Liu; Xiao Liu; Yuan-Qiao Cao; Ming Liu; Miao-Miao Han; Sheng-Xiang Ji

doi:10.1007/s10118-022-2859-1

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Engineering Antibacterial Activities and Biocompatibility of Hyperbranched Lysine-based Random Copolymers

RESEARCH ARTICLE | Updated：2023-01-18

- Engineering Antibacterial Activities and Biocompatibility of Hyperbranched Lysine-based Random Copolymers
- Engineering Antibacterial Activities and Biocompatibility of Hyperbranched Lysine-based Random Copolymers
- 高分子科学（英文版） 2023年41卷第3期页码：345-355
- Affiliations：
  
  a.Key Laboratory of Polymer Ecomaterials, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China
  b.University of Science and Technology of China, Hefei 230026, China
- Author bio：
  
  hanmiaomiao@ciac.ac.cn (M.M.H.)
  sji@ciac.ac.cn (S.X.J.)
- Funds：
  
  This work was financially supported by the National Natural Science Foundation of China (No. 51973212) and Department of Science and Technology of Jilin Province (Nos. 20210203119SF and 20210203173SF).;Electronic supplementary information (ESI) is available free of charge in the online version of this article at http://doi.org/10.1007/s10118-022-2859-1.
- DOI：10.1007/s10118-022-2859-1
  中图分类号：
- 收稿：2022-07-18，
  
  修回：2022-8-18，
  
  录用：2022-8-23，
  
  网络首发：2022-10-20，
  
  纸质出版：2023-03-01
- Accepted：
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Liu, H.; Liu, X.; Cao, Y. Q.; Liu, M.; Han, M. M.; Ji, S. X. Engineering antibacterial activities and biocompatibility of hyperbranched lysine-based random copolymers. Chinese J. Polym. Sci. 2023, 41, 345–355

Hui Liu, Xiao Liu, Yuan-Qiao Cao, et al. Engineering Antibacterial Activities and Biocompatibility of Hyperbranched Lysine-based Random Copolymers[J]. Chinese Journal of Polymer Science, 2023, 41(3): 345-355.
Liu, H.; Liu, X.; Cao, Y. Q.; Liu, M.; Han, M. M.; Ji, S. X. Engineering antibacterial activities and biocompatibility of hyperbranched lysine-based random copolymers. Chinese J. Polym. Sci. 2023, 41, 345–355 DOI： 10.1007/s10118-022-2859-1.

Hui Liu, Xiao Liu, Yuan-Qiao Cao, et al. Engineering Antibacterial Activities and Biocompatibility of Hyperbranched Lysine-based Random Copolymers[J]. Chinese Journal of Polymer Science, 2023, 41(3): 345-355. DOI： 10.1007/s10118-022-2859-1.

摘要

The toxicity of hyperbranched poly(lysine) (HPL) was engineered by copolymerizing lysine with hydrophobic amino acids such as alanine

tryptophan

or phenylalanine to obtain hyperbranched random copolymers. These copolymers have comparable antibacterial activities to HPL while their cytotoxicities and in vivo toxicities are lowered.

Abstract

Antimicrobial peptides (AMPs) have been considered as an alternative to small molecule antibiotics since they are difficult to develop antimicrobial resistance. Hyperbranched polylysine (HPL)

an AMP mimics

has gained attention due to its broad-spectrum antibacterial activities

but it also suffers from high toxicity. Here we report a facile strategy to engineer the toxicity of HPL by copolymerizing lysine (K) with a hydrophobic amino acid

e.g.

alanine (A)

tryptophan (W) or phenylalanine (F)

to afford hyperbranched random copolymers. These copolymers have comparable antibacterial activities to HPL while their cytotoxicities and

in vivo

toxicities are lowered when the type and content of hydrophobic amino acid and the size of copolymers are optimized. The

G. mellonella

infection model demonstrates that the copolymers are effective against the

S. aureus

infection

in vivo

. The copolymers kill the bacteria through the disruption of cell membranes and the bacteria do not develop resistance to the copolymers.

关键词

Keywords

references

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