An Effective Platform for SARS-CoV-2 Prevention by Combining Neutralization and RNAi Technology

Jun-Xiang Zhou; Xue Yin; Hao-Jun Liang

doi:10.1007/s10118-022-2846-6

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An Effective Platform for SARS-CoV-2 Prevention by Combining Neutralization and RNAi Technology

RESEARCH ARTICLE | Updated：2023-01-18

- An Effective Platform for SARS-CoV-2 Prevention by Combining Neutralization and RNAi Technology
- An Effective Platform for SARS-CoV-2 Prevention by Combining Neutralization and RNAi Technology
- 高分子科学（英文版） 2023年41卷第3期页码：327-333
- Affiliations：
  
  Department of Polymer Science and Engineering, Hefei National Research Center for Physical Sciences at the Microscale, iChEM (Collaborative Innovation Center of Chemistry for Energy Materials), University of Science and Technology of China, Hefei 230026, China
- Author bio：
  
  hjliang@ustc.edu.cn
- Funds：
  
  This work was financially supported by the National Natural Science Foundation of China (Nos. 21991132, 52033010 and 52021002), the National Key R&D Program of China (Nos. 2020YFA0710700 and 2020YFA0710703), the fundamental Research funds for the central universities (No. WK9110000005), and the Open Research Fund of State Key Laboratory of Polymer Physics and Chemistry (Changchun Institute of Applied Chemistry, Chinese Academy of Sciences).;Electronic supplementary information (ESI) is available free of charge in the online version of this article at http://doi.org/10.1007/s10118-022-2846-6.
- DOI：10.1007/s10118-022-2846-6
  中图分类号：
- 收稿：2022-06-21，
  
  修回：2022-7-17，
  
  录用：2022-7-18，
  
  网络首发：2022-09-21，
  
  纸质出版：2023-03-01
- Accepted：
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Zhou, J. X.; Yin, X.; Liang, H. J. An effective platform for SARS-CoV-2 prevention by combining neutralization and RNAi technology. Chinese J. Polym. Sci. 2023, 41, 327–333

Jun-Xiang Zhou, Xue Yin, Hao-Jun Liang. An Effective Platform for SARS-CoV-2 Prevention by Combining Neutralization and RNAi Technology[J]. Chinese Journal of Polymer Science, 2023, 41(3): 327-333.
Zhou, J. X.; Yin, X.; Liang, H. J. An effective platform for SARS-CoV-2 prevention by combining neutralization and RNAi technology. Chinese J. Polym. Sci. 2023, 41, 327–333 DOI： 10.1007/s10118-022-2846-6.

Jun-Xiang Zhou, Xue Yin, Hao-Jun Liang. An Effective Platform for SARS-CoV-2 Prevention by Combining Neutralization and RNAi Technology[J]. Chinese Journal of Polymer Science, 2023, 41(3): 327-333. DOI： 10.1007/s10118-022-2846-6.

摘要

A novel platform was established for prevention against the SARS-CoV-2 by utilizing a customized cell line. The pseudotyped SARS-CoV-2 was efficienly captured by the overexpressed ACE2 on the cell membrane. The subsequent viral replication and transcription processes could be blocked by the intracellular shRNAs designed as comlement to the viral genome fragments.

Abstract

At present

the coronavirus disease 2019 (COVID-19) pandemic is a global health crisis. Scientists all over the globe are urgently looking forward to an effective solution to prevent the spread of the epidemic and avoid more casualties at an early date. In this study

we establish an effective platform for the prevention of SARS-CoV-2 by combining the neutralization strategy and RNAi technology. To protect normal cells from infection

the customized cells are constructed to stably express viral antigenic receptor ACE2 on the cell membrane. These modified cells are used as bait for inducing the viral entry. The transcription and replication activities of viral genome are intercepted subsequently by the intracellular shRNAs

which are complementary to the viral gene fragments. A pseudotyped virus reconstructed from the HIV lentivirus is utilized as a virus model

by which we validate the feasibility and effectiveness of our strategy

in vitro

. Our work establishes an initial model and lays the foundation for future prevention and treatment of various RNA viruses.

关键词

Keywords

references

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