A Macroporous Cryogel with Enhanced Mechanical Properties for Osteochondral Regeneration <italic style="font-style: italic">In vivo</italic>

Xin-Yu Wu; Jun Yang; Fang-Hui Wu; Wang-Bei Cao; Tong Zhou; Zhao-Yi Wang; Chen-Xi Tu; Zhong-Ru Gou; Lei Zhang; Chang-You Gao

doi:10.1007/s10118-022-2835-9

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A Macroporous Cryogel with Enhanced Mechanical Properties for Osteochondral Regeneration In vivo

RESEARCH ARTICLE | Updated：2022-12-19

- A Macroporous Cryogel with Enhanced Mechanical Properties for Osteochondral Regeneration In vivo
- A Macroporous Cryogel with Enhanced Mechanical Properties for Osteochondral Regeneration In vivo
- 高分子科学（英文版） 2023年41卷第1期页码：40-50
- Affiliations：
  
  a.Ministry of Education, Key Laboratory of Macromolecular Synthesis and Functionalization, Department of Polymer Science and Engineering, Zhejiang University, Hangzhou 310027, China
  b.Department of Orthopaedics, the Third Hospital Affiliated to Wenzhou Medical University & Rui’an People’s Hospital, Rui’an 325200, China
  c.Bio-nanomaterials and Regenerative Medicine Research Division, Zhejiang-California International Nanosystem Institute, Zhejiang University, Hangzhou 310058, China
- Author bio：
  
  cygao@zju.edu.cn
- Funds：
  
  We thank Dr. Zhiyang Yu at the College of Agriculture and Biotechnology, Institute of Nuclear-Agricultural Science, Zhejiang University, for the assistance with the irradiation sterilization assay by a high-energy electron accelerator. This work was financially supported by the Key research and development program of Zhejiang Province (No. 2021C03113) and the Natural Science Foundation of Zhejiang Province (No. LD21E030001).;Electronic supplementary information (ESI) is available free of charge in the online version of this article at http://doi.org/10.1007/s10118-022-2835-9.
- DOI：10.1007/s10118-022-2835-9
  中图分类号：
- 收稿：2022-06-01，
  
  修回：2022-6-27，
  
  录用：2022-7-2，
  
  网络首发：2022-10-08，
  
  纸质出版：2023-01-01
- Accepted：
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Wu, X. Y.; Yang, J.; Wu, F. H.; Cao, W. B.; Zhou, T.; Wang, Z. Y.; Tu, C. X.; Gou, Z. R.; Zhang, L.; Gao, C. Y. A macroporous cryogel with enhanced mechanical properties for osteochondral regeneration in vivo. Chinese J. Polym. Sci. 2023, 41, 40–50

Xin-Yu Wu, Jun Yang, Fang-Hui Wu, et al. A Macroporous Cryogel with Enhanced Mechanical Properties for Osteochondral Regeneration In vivo[J]. Chinese Journal of Polymer Science, 2023, 41(1): 40-50. DOI: 10.1007/s10118-022-2835-9.
Wu, X. Y.; Yang, J.; Wu, F. H.; Cao, W. B.; Zhou, T.; Wang, Z. Y.; Tu, C. X.; Gou, Z. R.; Zhang, L.; Gao, C. Y. A macroporous cryogel with enhanced mechanical properties for osteochondral regeneration in vivo. Chinese J. Polym. Sci. 2023, 41, 40–50 DOI： 10.1007/s10118-022-2835-9.

Xin-Yu Wu, Jun Yang, Fang-Hui Wu, et al. A Macroporous Cryogel with Enhanced Mechanical Properties for Osteochondral Regeneration In vivo[J]. Chinese Journal of Polymer Science, 2023, 41(1): 40-50. DOI: 10.1007/s10118-022-2835-9. DOI：

摘要

A novel cell-free hydrogel scaffold with macroporous structure and enhanced mechanical properties is designed and fabricated by using two gelatin derivatives to regenerate osteochondral defect

in situ

Abstract

The dense networks of conventional hydrogel hinder the efficient cell penetration and thus tissue regeneration. In this study

a macroporous cryogel with excellent mechanical properties was designed and fabricated through cryogelation and crosslinking of methacrylated gelatin (Gel-MA) and gelatin grafted with 2-ureido-4[1

]-6-methyl-pyrimidinone (Gel-UPY). The successful modification of the two functional units on the side chain of gelatin was confirmed by

H-NMR and infrared spectroscopy. The macroporous structure of cryogel was verified by scanning electron microscopy and confocal microscopy. The compressive modulus of cryogel (110 kPa) was 2 magnitudes higher than that of the conventional hydrogel of the same chemical compositions (8 kPa). Both the hydrogel and cryogel exhibited excellent stress-recovery ability. The cryogel could well maintain the viability of chondrocytes and facilitated their migration into the scaffold interior. After being implanted into osteochondral defects in rabbits for 12 w

the Gel-MA/Gel-UPY cryogel better facilitated the regeneration of upper cartilage and subchondral bone compared to the conventional hydrogel.

关键词

Keywords

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A Macroporous Cryogel with Enhanced Mechanical Properties for Osteochondral Regeneration In vivo

DOI：10.1007/s10118-022-2835-9

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