Mesenchymal Stem Cells Proliferation on Konjac Glucomannan Microcarriers: Effect of Rigidity

Xing-Ran Yan; Juan Li; Xiang-Ming Na; Tong Li; Yu-Fei Xia; Wei-Qing Zhou; Guang-Hui Ma

doi:10.1007/s10118-022-2800-7

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Mesenchymal Stem Cells Proliferation on Konjac Glucomannan Microcarriers: Effect of Rigidity

RESEARCH ARTICLE | Updated：2022-08-19

- Mesenchymal Stem Cells Proliferation on Konjac Glucomannan Microcarriers: Effect of Rigidity
- Mesenchymal Stem Cells Proliferation on Konjac Glucomannan Microcarriers: Effect of Rigidity
- 高分子科学（英文版） 2022年40卷第9期页码：1080-1089
- Affiliations：
  
  a.State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China
  b.University of Chinese Academy of Sciences, Beijing 100049, China
- Author bio：
  
  wqzhou@ipe.ac.cn (W.Q.Z.)
  ghma@ipe.ac.cn (G.H.M.)
- Funds：
  
  This work was financially supported by the Strategic Priority Research Program of the Chinese Academy of Sciences (No. XDA16020405), National Key Research and Development Program (2020YFA0112603), the National Natural Science Foundation of China (Nos. 21902160 and 21821005).
- DOI：10.1007/s10118-022-2800-7
  中图分类号：
- 收稿：2022-04-01，
  
  修回：2022-05-11，
  
  录用：2022-05-20，
  
  网络首发：2022-07-12，
  
  纸质出版：2022-09-01
- Accepted：
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Yan, X. R.; Li, J.; Na, X. M.; Li, T.; Xia, Y. F.; Zhou, W. Q.; Ma, G. H. Mesenchymal stem cells proliferation on konjac glucomannan microcarriers: effect of rigidity. Chinese J. Polym. Sci. 2022, 40, 1080–1089

Xing-Ran Yan, Juan Li, Xiang-Ming Na, et al. Mesenchymal Stem Cells Proliferation on Konjac Glucomannan Microcarriers: Effect of Rigidity[J]. Chinese Journal of Polymer Science, 2022, 40(9): 1080-1089.
Yan, X. R.; Li, J.; Na, X. M.; Li, T.; Xia, Y. F.; Zhou, W. Q.; Ma, G. H. Mesenchymal stem cells proliferation on konjac glucomannan microcarriers: effect of rigidity. Chinese J. Polym. Sci. 2022, 40, 1080–1089 DOI： 10.1007/s10118-022-2800-7.

Xing-Ran Yan, Juan Li, Xiang-Ming Na, et al. Mesenchymal Stem Cells Proliferation on Konjac Glucomannan Microcarriers: Effect of Rigidity[J]. Chinese Journal of Polymer Science, 2022, 40(9): 1080-1089. DOI： 10.1007/s10118-022-2800-7.

摘要

MSCs exhibit rapid proliferation and differentiation potential maintenance on microcarriers with intermediate rigidity which stimulates the fastest spreading rate.

Abstract

Mesenchymal stem cells (MSCs) have attracted great attention as a source of cells for regenerative medicine owing to their self-renewal ability and multiple differentiation potential. However

the serious shortage of MSCs and the difficulty of maintaining the differentiation potential during culture

in vitro

limit their application. Microcarrier culture technology is an effective method to realize large-scale culture of MSCs

whereas the effect of microcarriers properties on MSCs proliferation and differentiation potential maintenance should be investigated. In this study

Konjac glucomannan (KGM) microcarriers with a wide range of rigidity from 0.2 MPa to 10 MPa were prepared using water-in-oil emulsion polymerization. It was found that the microcarriers rigidity had great influence on MSCs by regulating cell spreading rate. Rapid proliferation and good differentiation potential maintenance of MSCs were achieved on the intermediate rigidity about 2.51±0.65 MPa with the faster spreading rate of cells. As a result

MSCs on 2.51±0.65 MPa KGM microcarriers proliferated about 27 times

which was 1.7 times as much as MSCs cultured on the commercial microcarriers Cytodex-1. The differentiation potential was also improved about 3.2 times compared to Cytodex-1. Therefore

it is indispensable to regulate microcarriers rigidity

especially for application of MSCs.

关键词

Keywords

references

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