Construction of Magnetic Resonance Imaging Visible Polymeric Vector for Efficient Tumor Targeted siRNA Delivery

Rong-Ze Wang; Si Huang; Qiao-Yun Zhang; Xing-Su Yu; Ke-Ze Hong; Jian-Rong Cao; Hong Xiao; Yong Wang; Xin-Tao Shuai

doi:10.1007/s10118-022-2794-1

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Construction of Magnetic Resonance Imaging Visible Polymeric Vector for Efficient Tumor Targeted siRNA Delivery

RESEARCH ARTICLE | Updated：2022-08-19

- Construction of Magnetic Resonance Imaging Visible Polymeric Vector for Efficient Tumor Targeted siRNA Delivery
- Construction of Magnetic Resonance Imaging Visible Polymeric Vector for Efficient Tumor Targeted siRNA Delivery
- 高分子科学（英文版） 2022年40卷第9期页码：1071-1079
- Affiliations：
  
  a.College of Chemistry and Materials Science, Jinan University, Guangzhou 510632, China
  b.Nanomedicine Research Center, The Third Affiliated Hospital, Sun Yat-sen University, Guangzhou 510630, China
  c.Department of Reproductive Medical Center, Guangdong Women and Children Hospital, Guangzhou 511400, China
- Author bio：
  
  wangy488@jnu.edu.cn (Y.W.)
  shuaixt@mail.sysu.edu.cn (X.T.S.)
- Funds：
  
  We thank the members of the Shuai’s laboratories for helpful discussion and technical assistance. This work was financially supported by the National Natural Science Foundation of China (Nos. 52173125 and 21805314), the Key Areas Research and Development Program of Guangzhou (No. 202007020006), and Natural Science Foundation of the Guangdong Province (No. 2021A1515010250).
- DOI：10.1007/s10118-022-2794-1
  中图分类号：
- 收稿：2022-04-01，
  
  修回：2022-05-01，
  
  录用：2022-05-07，
  
  网络首发：2022-07-12，
  
  纸质出版：2022-09-01
- Accepted：
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Wang, R. Z.; Huang, S.; Zhang, Q. Y.; Yu, X. S.; Hong, K. Z.; Cao, J. R.; Xiao, H.; Wang, Y.; Shuai, X. T. Construction of magnetic resonance imaging visible polymeric vector for efficient tumor targeted siRNA delivery. Chinese J. Polym. Sci. 2022, 40, 1071–1079

Rong-Ze Wang, Si Huang, Qiao-Yun Zhang, et al. Construction of Magnetic Resonance Imaging Visible Polymeric Vector for Efficient Tumor Targeted siRNA Delivery[J]. Chinese Journal of Polymer Science, 2022, 40(9): 1071-1079.
Wang, R. Z.; Huang, S.; Zhang, Q. Y.; Yu, X. S.; Hong, K. Z.; Cao, J. R.; Xiao, H.; Wang, Y.; Shuai, X. T. Construction of magnetic resonance imaging visible polymeric vector for efficient tumor targeted siRNA delivery. Chinese J. Polym. Sci. 2022, 40, 1071–1079 DOI： 10.1007/s10118-022-2794-1.

Rong-Ze Wang, Si Huang, Qiao-Yun Zhang, et al. Construction of Magnetic Resonance Imaging Visible Polymeric Vector for Efficient Tumor Targeted siRNA Delivery[J]. Chinese Journal of Polymer Science, 2022, 40(9): 1071-1079. DOI： 10.1007/s10118-022-2794-1.

摘要

We propose a cationic block polymer based on polyethylene glycol and poly aspartic acid for siRNA delivery. The vector exerts multi properties including good biocompatibility

high encapsulation efficiency

and tailorable modification for superparamagnetic iron oxide and targeting components

which result in a highly efficient and tumor targeted siRNA delivery.

Abstract

RNA interference (RNAi)

known for the highly efficient targeted gene silencing

has been demonstrated to be a promising means for cancer treatment. Meanwhile

an effective approach for siRNA delivery is urgently needed to meet the needs for its clinical application. Herein

we constructed a polymeric vector labeled with superparamagnetic iron oxide (SPIO) for magnetic resonance imaging (MRI) visible siRNA delivery. EGFR antibody was also modified to the surface of nanodrug to enhance the delivery effect. Our results showed that the vector exhibited great siRNA complexation ability and mediated an increased endocytosis of siRNA without obvious cytotoxicity. Besides

both

in vitro

and

in vivo

studies evidenced the vector could effectively deliver siRNA into tumor cells

exert highly interfering effect

and show potent MR imaging capacity. The study provides a promising MRI-visible and EGFR targeting delivery system to improve RNAi efficacy for cancer therapy.

关键词

Keywords

references

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