Antibacterial Sutures Coated with Smooth Chitosan Layer by Gradient Deposition

Ying-Ge Chen; Chu-Xin Li; Yu Zhang; Yong-Dan Qi; Jun Feng; Xian-Zheng Zhang

doi:10.1007/s10118-022-2770-9

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Antibacterial Sutures Coated with Smooth Chitosan Layer by Gradient Deposition

RESEARCH ARTICLE | Updated：2022-08-19

- Antibacterial Sutures Coated with Smooth Chitosan Layer by Gradient Deposition
- Antibacterial Sutures Coated with Smooth Chitosan Layer by Gradient Deposition
- 高分子科学（英文版） 2022年40卷第9期页码：1050-1061
- Affiliations：
  
  Key Laboratory of Biomedical Polymers of Ministry of Education & Department of Chemistry, Wuhan University, Wuhan 430072, China
- Author bio：
  
  fengjun@whu.edu.cn (J.F.)
  xz-zhang@whu.edu.cn (X.Z.Z.)
- Funds：
  
  This work was financially supported by the National Natural Science Foundation of China (Nos. 51973164, 52131302, 22135005 and 51833007), National Key Research and Development Program of China (2019YFA0905603) and Fundamental Research Funds for the Central Universities (No. 2042021kf0037). All of the animal experiments were conducted under protocols approved by the Institutional Animal Care and Use Committee (IACUC) of the Animal Experiment Center of Wuhan University (Wuhan, P. R. China).
- DOI：10.1007/s10118-022-2770-9
  中图分类号：
- 收稿：2022-03-30，
  
  修回：2022-04-20，
  
  录用：2022-05-09，
  
  网络首发：2022-07-12，
  
  纸质出版：2022-09-01
- Accepted：
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Chen, Y. G.; Li, C. X.; Zhang, Y.; Qi, Y. D.; Feng, J.; Zhang, X. Z. Antibacterial sutures coated with smooth chitosan layer by gradient deposition. Chinese J. Polym. Sci. 2022, 40, 1050–1061

Ying-Ge Chen, Chu-Xin Li, Yu Zhang, et al. Antibacterial Sutures Coated with Smooth Chitosan Layer by Gradient Deposition[J]. Chinese Journal of Polymer Science, 2022, 40(9): 1050-1061.
Chen, Y. G.; Li, C. X.; Zhang, Y.; Qi, Y. D.; Feng, J.; Zhang, X. Z. Antibacterial sutures coated with smooth chitosan layer by gradient deposition. Chinese J. Polym. Sci. 2022, 40, 1050–1061 DOI： 10.1007/s10118-022-2770-9.

Ying-Ge Chen, Chu-Xin Li, Yu Zhang, et al. Antibacterial Sutures Coated with Smooth Chitosan Layer by Gradient Deposition[J]. Chinese Journal of Polymer Science, 2022, 40(9): 1050-1061. DOI： 10.1007/s10118-022-2770-9.

摘要

We developed a "gradient deposition" technique to coat a continuous and smooth layer of chitosan onto the surface of absorbable sutures. The coated sutures exhibited excellent anti-bacterial and anti-inflammation activities for significantly enhanced wound healing effect.

Abstract

Owing to the significant importance in clinics

antibacterial activity is thought as one indispensable feature of the next generation of absorbable sutures. It is challenging but imperative to arm the existing absorbable sutures with antibacterial functions. The present study describes a "gradient deposition" technique to coat a continuous and smooth layer of chitosan on the surface of absorbable sutures. Specifically

chitosan solution is arranged to undergo gradient pH decline step by step while during each pH interval

the solution is allowed to stand for a predetermined period of time in order to control gradual chitosan deposition. Chitosan nanoparticles are found to be first generated on suture surface and finally developed into a smooth chitosan layer as the antibacterial surface.

In vitro

and

in vivo

results demonstrated that coating chitosan on sutures by our technique could relieve wound inflammation

stimulate collagen deposition

regenerate blood vessels

and assist tissue repairing

consequently leading to a significant enhancement of wound healing effect. This technique is highlighted with low cost

extreme convenience and excellent safety without organic solvents. Furthermore

the "gradient deposition" technique would not affect the fundamental properties of matrix and thus hold promises as a universal way for superficial antibacterial modification towards almost all the surgical implanted materials

including but not limited to absorbable sutures.

关键词

Keywords

references

Dennis, C.; Sethu, S.; Nayak, S.; Mohan, L.; Morsi, Y.; Manivasagam, G . Suture materials—current and emerging trends . J. Biomed. Mater. Res. A , 2016 . 104 1544 - 1559 . DOI: 10.1002/jbm.a.35683 http://doi.org/10.1002/jbm.a.35683 .

de la Harpe, K. M.; Kondiah, P. P. D.; Marimuthu, T.; Choonara, Y. E . Advances in carbohydrate-based polymers for the design of suture materials: a review . Carbohydr. Polym. , 2021 . 261 117860 DOI: 10.1016/j.carbpol.2021.117860 http://doi.org/10.1016/j.carbpol.2021.117860 .

Pillai, C. K. S.; Sharma, C. P . Review paper: absorbable polymeric surgical sutures: chemistry, production, properties, biodegradability, and performance . J. Biomater. Appl. , 2010 . 25 291 - 366 . DOI: 10.1177/0885328210384890 http://doi.org/10.1177/0885328210384890 .

Ashraf, I.; Butt, E.; Veitch, D.; Wernham, A. Dermatological surgery: an update on suture materials and techniques . Part 1 . Clin. Exp. Dermatol. , 2021 . 46 1400 - 1410 . DOI: 10.1111/ced.14770 http://doi.org/10.1111/ced.14770 .

Abhari, R. E.; Martins, J. A.; Morris, H . L.; Mouthuy, P. A.; Carr, A. Synthetic sutures: clinical evaluation and future developments . J. Biomater. Appl. , 2017 . 32 410 - 421 . DOI: 10.1177/0885328217720641 http://doi.org/10.1177/0885328217720641 .

Taylor, J. S.; Marten, C. A.; Potts, K. A.; Cloutier, L. M.; Cain, K. E.; Fenton, S. L.; Tatum, T. N.; James, D. A.; Myers, K. N.; Hubbs, C. A.; Burzawa, J. K.; Vachhani, S.; Nick, A. M.; Meyer, L. A.; Graviss, L. S.; Ware, K. M.; Park, A. K.; Aloia, T. A.; Bodurka, D. C.; Levenback, C. F.; Schmeler, K. M . What is the real rate of surgical site infection? . J. Oncol. Pract. , 2016 . 12 e878 - e883 . DOI: 10.1200/JOP.2016.011759 http://doi.org/10.1200/JOP.2016.011759 .

Wagner, J. C.; Wetz, A.; Wiegering, A.; Lock, J . F.; Löb, S.; Germer, C. T.; Klein, I. Successful surgical closure of infected abdominal wounds following preconditioning with negative pressure wound therapy . Langenbeck. Arch. Surg. , 2021 . 406 2479 - 2487 . DOI: 10.1007/s00423-021-02221-w http://doi.org/10.1007/s00423-021-02221-w .

Renko, M.; Paalanne, N. Tapiainen, T.; Hinkkainen, M.; Pokka, T.; Kinnula, S.; Sinikumpu, J . J.; Uhari, M.; Serlo, W. Triclosan-containing sutures versus ordinary sutures for reducing surgical site infections in children: a double-blind, randomised controlled trial . Lancet. Infect. Dis. , 2017 . 17 50 - 57 . DOI: 10.1016/S1473-3099(16)30373-5 http://doi.org/10.1016/S1473-3099(16)30373-5 .

Tummalapalli, M.; Anjum, S.; Kumari, S.; Gupta, B . Antimicrobial surgical sutures: recent developments and strategies . Polym. Rev. , 2016 . 56 607 - 630 . DOI: 10.1080/15583724.2015.1119163 http://doi.org/10.1080/15583724.2015.1119163 .

Fields, A. C.; Pradarelli, J. C.; Itani, K. M. F . Preventing surgical site infections: looking beyond the current guidelines . JAMA , 2020 . 323 1087 - 1088 . DOI: 10.1001/jama.2019.20830 http://doi.org/10.1001/jama.2019.20830 .

Wu, X.; Kubilay, N . Z.; Ren, J.; Allegranzi, B.; Bischoff, P.; Zayed, B.; Pittet, D.; Li, J. Antimicrobial-coated sutures to decrease surgical site infections: a systematic review and meta-analysis . Eur. J. Clin. Microbiol. , 2017 . 36 19 - 32 . DOI: 10.1007/s10096-016-2765-y http://doi.org/10.1007/s10096-016-2765-y .

Deng, X.; Qasim, M.; Ali, A . Engineering and polymeric composition of drug-eluting suture: a review . J. Biomed. Mater. Res. A , 2021 . 109 2065 - 2081 . DOI: 10.1002/jbm.a.37194 http://doi.org/10.1002/jbm.a.37194 .

Alshomer, F.; Madhavan, A.; Pathan, O.; Song, W . Bioactive sutures: a review of advances in surgical suture functionalisation . Curr. Med. Chem. , 2017 . 24 215 - 223 . DOI: 10.2174/0929867324666161118141724 http://doi.org/10.2174/0929867324666161118141724 .

Lee, J.; Yoo, J.; Kim, J.; Jang, Y.; Shin, K.; Ha, E.; Ryu, S.; Kim, B. G.; Wooh, S.; Char, K . Development of multimodal antibacterial surfaces using porous amine-reactive films incorporating lubricant and silver nanoparticles . ACS Appl. Mater. Interfaces , 2019 . 11 6550 - 6560 . DOI: 10.1021/acsami.8b20092 http://doi.org/10.1021/acsami.8b20092 .

Wang, X.; Liu, P.; Wu, Q.; Zheng, Z.; Xie, M.; Chen, G.; Yu, J.; Wang, X.; Li, G.; Kaplan, D . Sustainable antibacterial and anti-inflammatory silk suture with surface modification of combined-therapy drugs for surgical site infection . ACS Appl. Mater. Interfaces , 2022 . 14 11177 - 11191 . DOI: 10.1021/acsami.2c00106 http://doi.org/10.1021/acsami.2c00106 .

Serrano, C.; García-Fernández, L.; Fernández-Blázquez, J . P.; Barbeck, M.; Ghanaati, S.; Unger, R.; Kirkpatrick, J.; Arzt, E.; Funk, L.; Turón, P.; del Campo, A. Nanostructured medical sutures with antibacterial properties . Biomaterials , 2015 . 52 291 - 300 . DOI: 10.1016/j.biomaterials.2015.02.039 http://doi.org/10.1016/j.biomaterials.2015.02.039 .

Benesch, J.; Tengvall, P . Blood protein adsorption onto chitosan . Biomaterials , 2002 . 23 2561 - 2568 . DOI: 10.1016/S0142-9612(01)00391-X http://doi.org/10.1016/S0142-9612(01)00391-X .

Kim, U. J.; Lee, Y. R.; Kang, T. H.; Choi, J . W.; Kimura, S.; Wada, M. Protein adsorption of dialdehyde cellulose-crosslinked chitosan with high amino group contents . Carbohydr. Polym. , 2017 . 163 34 - 42 . DOI: 10.1016/j.carbpol.2017.01.052 http://doi.org/10.1016/j.carbpol.2017.01.052 .

Shi, X. W.; Li, X. X.; Du, Y. M . Recent progress of chitin-based materials . Acta Polymerica Sinica (in Chinese) , 2011 . 1 - 17 . DOI: 10.3724/SP.J.1105.2011.10239 http://doi.org/10.3724/SP.J.1105.2011.10239 .

Xia, G. X.; Wu, Y. M.; Bi, Y. F.; Chen, K.; Zhang, W. W.; Liu, S. Q.; Zhang, W. J.; Liu, R. H . Antimicrobial properties and application of polysaccharides and their derivatives . Chinese J. Polym. Sci. , 2021 . 39 133 - 146 . DOI: 10.1007/s10118-021-2506-2 http://doi.org/10.1007/s10118-021-2506-2 .

Li, J.; Wu, Y.; Zhao, L . Antibacterial activity and mechanism of chitosan with ultra high molecular weight . Carbohydr. Polym. , 2016 . 148 200 - 205 . DOI: 10.1016/j.carbpol.2016.04.025 http://doi.org/10.1016/j.carbpol.2016.04.025 .

Benchamas, G.; Huang, G.; Huang, S.; Huang, H . Preparation and biological activities of chitosan oligosaccharides . Trends Food. Sci. Tech. , 2021 . 107 38 - 44 . DOI: 10.1016/j.jpgs.2020.11.027 http://doi.org/10.1016/j.jpgs.2020.11.027 .

Shariatinia, Z . Pharmaceutical applications of chitosan . Adv. Colloid Interface Sci. , 2019 . 263 131 - 194 . DOI: 10.1016/j.cis.2018.11.008 http://doi.org/10.1016/j.cis.2018.11.008 .

Ghasemzadeh, H.; Sheikhahmadi, M.; Nasrollah, F . Full polysaccharide crosslinked-chitosan and silver nano composites, for use as an antibacterial membrane . Chinese J. Polym. Sci. , 2016 . 34 949 - 964 . DOI: 10.1007/s10118-016-1807-3 http://doi.org/10.1007/s10118-016-1807-3 .

Avcu, E.; Baştan, F. E.; Abdullah, H. Z.; Rehman, M. A. U.; Avcu, Y. Y.; Boccaccini, A. R . Electrophoretic deposition of chitosan-based composite coatings for biomedical applications: a review . Prog. Mater. Sci. , 2019 . 103 69 - 108 . DOI: 10.1016/j.pmatsci.2019.01.001 http://doi.org/10.1016/j.pmatsci.2019.01.001 .

Boon-in, S.; Theerasilp, M.; Crespy, D. . Marrying the incompatible for better: Incorporation of hydrophobic payloads in superhydrophilic hydrogels . J. Colloid Interface Sci. , 2022 . 622 75 - 86 . DOI: 10.1016/j.jcis.2022.04.029 http://doi.org/10.1016/j.jcis.2022.04.029 .

Abdelkader, A.; Fathi, H. A.; Hamad, M. A.; Elsabahy, M . Nanomedicine: a new paradigm to overcome drug incompatibilities . J. Pharm. Pharmacol. , 2020 . 72 1289 - 1305 . DOI: 10.1111/jphp.13292 http://doi.org/10.1111/jphp.13292 .

Qin, B.; Fei, C.; Bridges Andrew, A.; Mashruwala Ameya, A.; Stone Howard, A.; Wingreen Ned, S.; Bassler Bonnie, L . Cell position fates and collective fountain flow in bacterial biofilms revealed by light-sheet microscopy . Science , 2020 . 369 71 - 77 . DOI: 10.1126/science.abb8501 http://doi.org/10.1126/science.abb8501 .

Ciofu, O.; Moser, C.; Jensen, P. O.; Hoiby, N. Tolerance and resistance of microbial biofilms. Nat. Rev. Microbiol. 2022 , DOI: 10.1038/s41579-022-00682-4

Hall-Stoodley, L.; Costerton, J. W.; Stoodley, P . Bacterial biofilms: from the natural environment to infectious diseases . Nat. Rev. Microbiol. , 2004 . 2 95 - 108 . DOI: 10.1038/nrmicro821 http://doi.org/10.1038/nrmicro821 .

Mahesh, L.; Kumar, V. R.; Jain, A.; Shukla, S.; Aragoneses, J. M.; Martínez González, J. M.; Fernández-Domínguez, M.; Calvo-Guirado, J. L. . Bacterial adherence around sutures of different material at grafted site: a microbiological analysis . Materials , 2019 . 12 2848 DOI: 10.3390/ma12182848 http://doi.org/10.3390/ma12182848 .

Katz, S.; Izhar, M.; Mirelman, D . Bacterial adherence to surgical sutures: a possible factor in suture induced infection . Ann. Surg. , 1981 . 194 35 - 41 . DOI: 10.1097/00000658-198107000-00007 http://doi.org/10.1097/00000658-198107000-00007 .

Ali-Mucheru, M. N.; Seville, M. T.; Miller, V.; Sampathkumar, P.; Etzioni, D. A . Postoperative surgical site infections: understanding the discordance between surveillance systems . Ann. Surg. , 2020 . 271 94 - 99 . DOI: 10.1097/SLA.0000000000002780 http://doi.org/10.1097/SLA.0000000000002780 .

Wills, B. W.; Smith, W. R.; Arguello, A. M.; McGwin, G.; Ghanem, E. S.; Ponce, B. A . Association of surgical jacket and bouffant use with surgical site infection risk . JAMA Surg. , 2020 . 155 323 - 328 . DOI: 10.1001/jamasurg.2019.6044 http://doi.org/10.1001/jamasurg.2019.6044 .

Alverdy, J . C.; Hyman, N.; Gilbert, J. Re-examining causes of surgical site infections following elective surgery in the era of asepsis . Lancet. Infect. Dis. , 2020 . 20 e38 - e43 . .

Tomihata, K.; Suzuki, M.; Oka, T.; Ikada, Y . A new resorbable monofilament suture . Polym. Degrad. Stab. , 1998 . 59 13 - 18 . DOI: 10.1016/S0141-3910(97)00183-3 http://doi.org/10.1016/S0141-3910(97)00183-3 .

Hu, W.; Huang, Z. M . Biocompatibility of braided poly(L-lactic acid) nanofiber wires applied as tissue sutures . Polym. Int. , 2010 . 59 92 - 99 . DOI: 10.1002/pi.2695 http://doi.org/10.1002/pi.2695 .

Baygar, T.; Sarac, N.; Ugur, A.; Karaca, I. R . Antimicrobial characteristics and biocompatibility of the surgical sutures coated with biosynthesized silver nanoparticles . Bioorg. Chem. , 2019 . 86 254 - 258 . DOI: 10.1016/j.bioorg.2018.12.034 http://doi.org/10.1016/j.bioorg.2018.12.034 .

Korntner, S.; Lehner, C.; Gehwolf, R.; Wagner, A.; Grütz, M.; Kunkel, N.; Tempfer, H.; Traweger, A . Limiting angiogenesis to modulate scar formation . Adv. Drug Deliver. Rev. , 2019 . 146 170 - 189 . DOI: 10.1016/j.addr.2018.02.010 http://doi.org/10.1016/j.addr.2018.02.010 .

Konieczny, P.; Naik, S . Healing without scarring . Science , 2021 . 372 346 - 347 . DOI: 10.1126/science.abi5770 http://doi.org/10.1126/science.abi5770 .

Willenborg, S.; Eming Sabine, A . Cellular networks in wound healing . Science , 2018 . 362 891 - 892 . DOI: 10.1126/science.aav5542 http://doi.org/10.1126/science.aav5542 .

Rodrigues, M.; Kosaric, N.; Bonham, C. A.; Gurtner, G. C . Wound healing: a cellular perspective . Physiol. Rev. , 2018 . 99 665 - 706 . .

Ip, W. K . E.; Hoshi, N.; Shouval Dror, S.; Snapper, S.; Medzhitov, R. Anti-inflammatory effect of IL-10 mediated by metabolic reprogramming of macrophages . Science , 2017 . 356 513 - 519 . DOI: 10.1126/science.aal3535 http://doi.org/10.1126/science.aal3535 .

Sato, Y.; Ohshima, T.; Kondo, T . Regulatory role of endogenous Interleukin-10 in cutaneous inflammatory response of murine wound healing . Biochem. Biophys. Res. Commun. , 1999 . 265 194 - 199 . DOI: 10.1006/bbrc.1999.1455 http://doi.org/10.1006/bbrc.1999.1455 .

Sun, W.; Wu, Y.; Zheng, M.; Yang, Y.; Liu, Y.; Wu, C.; Zhou, Y.; Zhang, Y.; Chen, L.; Li, H . Discovery of an orally active small-molecule tumor necrosis factor- α inhibitor . J. Med. Chem. , 2020 . 63 8146 - 8156 . DOI: 10.1021/acs.jmedchem.0c00377 http://doi.org/10.1021/acs.jmedchem.0c00377 .

Yu, Q. J.; Fen, Z, C.; Huang, L. P.; He, J. W.; Zhou, Z. L.; Liu, F . Ellagic acid (EA), a tannin was isolated from Eucalyptus citriodora leaves and its anti-inflammatory activity . Med. Chem. Res. , 2021 . 30 2277 - 2288 . DOI: 10.1007/s00044-021-02806-6 http://doi.org/10.1007/s00044-021-02806-6 .

Johnson, B. Z.; Stevenson, A. W.; Prêle, C. M.; Fear, M. W.; Wood, F. M . The role of IL-6 in skin fibrosis and cutaneous wound healing . Biomedicines , 2020 . 8 101 - 119 . DOI: 10.3390/biomedicines8050101 http://doi.org/10.3390/biomedicines8050101 .

Leknes, K. N.; Selvig, K. A.; Boe, O. E.; Wikesjö, U. M. E . Tissue reactions to sutures in the presence and absence of anti-infective therapy . J. Clin. Periodontol. , 2005 . 32 130 - 138 . .

Masini, B. D.; Stinner, D. J.; Waterman, S. M.; Wenke, J. C . Bacterial adherence to suture materials . J. Surg. Educ. , 2011 . 68 101 - 104 . DOI: 10.1016/j.jsurg.2010.09.015 http://doi.org/10.1016/j.jsurg.2010.09.015 .

Markel, D . C.; Bergum, C.; Wu, B.; BouAkl, T.; Ren, W. Does suture type influence bacterial retention and biofilm formation after irrigation in a mouse model? . Clin. Orthop. Relat. Res. , 2019 . 477 116 - 126 . DOI: 10.1097/CORR.0000000000000391 http://doi.org/10.1097/CORR.0000000000000391 .

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