Tuning the Stability of the Polyplex Nanovesicles of Oligonucleotides <italic style="font-style: italic">via</italic> a Zinc(II)-Coordinative Strategy

Quan Zhou; Xiang Li; Jia-Jia Xiang; You-Qing Shen

doi:10.1007/s10118-022-2764-7

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Tuning the Stability of the Polyplex Nanovesicles of Oligonucleotides via a Zinc(II)-Coordinative Strategy

RESEARCH ARTICLE | Updated：2022-08-19

- Tuning the Stability of the Polyplex Nanovesicles of Oligonucleotides via a Zinc(II)-Coordinative Strategy
- Tuning the Stability of the Polyplex Nanovesicles of Oligonucleotides via a Zinc(II)-Coordinative Strategy
- 高分子科学（英文版） 2022年40卷第9期页码：1034-1042
- Affiliations：
  
  a.Zhejiang Key Laboratory of Smart Biomaterials and Center for Bionanoengineering, College of Chemical and Biological Engineering, Zhejiang University, Hangzhou 310027, China
  b.Department of Cell Biology, School of Basic Medical Sciences, Zhejiang University, Hangzhou 310058, China
  c.The Forth Affiliated Hospital, Zhejiang University School of Medicine, Yiwu 322000, China
  d.Hangzhou Global Scientific and Technological Innovation Center, Hangzhou 311215, China
- Author bio：
  
  shenyq@zju.edu.cn
- Funds：
  
  This work was financially supported by the National Key Research and Development Program of China (No. 2021YFA1201200), the National Natural Science Foundation of China (Nos. 51833008, 52173141 and 82102192), Zhejiang Provincial Key Research and Development Program (No. 2020C01123), and China Postdoctoral Science Foundation (No. 2019M662059).
- DOI：10.1007/s10118-022-2764-7
  中图分类号：
- 收稿：2022-03-08，
  
  修回：2022-04-03，
  
  录用：2022-04-15，
  
  网络首发：2022-07-04，
  
  纸质出版：2022-09-01
- Accepted：
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Zhou, Q.; Li, X.; Xiang, J. J.; Shen, Y. Q. Tuning the stability of the polyplex nanovesicles of oligonucleotides via a zinc(II)-coordinative strategy. Chinese J. Polym. Sci. 2022, 40, 1034–1042

Quan Zhou, Xiang Li, Jia-Jia Xiang, et al. Tuning the Stability of the Polyplex Nanovesicles of Oligonucleotides via a Zinc(II)-Coordinative Strategy[J]. Chinese Journal of Polymer Science, 2022, 40(9): 1034-1042. DOI: 10.1007/s10118-022-2764-7.
Zhou, Q.; Li, X.; Xiang, J. J.; Shen, Y. Q. Tuning the stability of the polyplex nanovesicles of oligonucleotides via a zinc(II)-coordinative strategy. Chinese J. Polym. Sci. 2022, 40, 1034–1042 DOI： 10.1007/s10118-022-2764-7.

Quan Zhou, Xiang Li, Jia-Jia Xiang, et al. Tuning the Stability of the Polyplex Nanovesicles of Oligonucleotides via a Zinc(II)-Coordinative Strategy[J]. Chinese Journal of Polymer Science, 2022, 40(9): 1034-1042. DOI: 10.1007/s10118-022-2764-7. DOI：

摘要

we here report a new methodology for tuning the saline stabilities of polyplex nanovesicles of oligonucleotides by modifying the polycation with Zinc (II)-coordinative cationic chains.

Abstract

Oligonucleotide therapeutics have great potential to target the currently undruggable genes and to generate entirely new therapeutic paradigms in multiple types of disease

thus having attracted much attention in recent years. However

their applications are greatly hindered by a lack of safe and efficient oligonucleotide-delivery vectors. Polyplex nanovesicles formed from oligonucleotides and the cationic block have shown exceptional features for the delivery of therapeutic oligonucleotides and other biopharmaceuticals. Nevertheless

these polyplex nanovesicles are deeply fraught with difficulty in tolerating physiological ionic strength. Inspired by the high binding ability between the dipicolylamine (DPA)/zinc(II) complex and the phosphodiester moieties of oligonucleotides

herein

we designed a coordinative cationic block to solve the intrinsic stability dilemma. Moreover

we found the stability of the resulted polyplex nanovesicles could be easily tuned by the content of coordinated zinc ions.

In vitro

cellular studies implied that the prepared zinc(II)-coordinative polyplex nanovesicles preferred to retain in the lysosomes upon internalization

making them ideal delivery candidates for the lysosome-targeting oligonucleotide therapeutics.

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Tuning the Stability of the Polyplex Nanovesicles of Oligonucleotides via a Zinc(II)-Coordinative Strategy

Tuning the Stability of the Polyplex Nanovesicles of Oligonucleotides via a Zinc(II)-Coordinative Strategy

DOI：10.1007/s10118-022-2764-7

摘要

Abstract

关键词

Keywords

references