Hydrogels with Dynamically Controllable Mechanics and Biochemistry for 3D Cell Culture Platforms

Hai-Yang Wu; Lei Yang; Jiang-Shan Tu; Jie Wang; Jin-Ge Li; Hong-Ying Lv; Xiao-Niu Yang

doi:10.1007/s10118-021-2639-3

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Hydrogels with Dynamically Controllable Mechanics and Biochemistry for 3D Cell Culture Platforms

Papers | Updated：2021-11-09

- Hydrogels with Dynamically Controllable Mechanics and Biochemistry for 3D Cell Culture Platforms
- Hydrogels with Dynamically Controllable Mechanics and Biochemistry for 3D Cell Culture Platforms
- Chinese Journal of Polymer Science 2022年40卷第1期页码：38-46
- Affiliations：
  
  a.State Key Laboratory of Polymer Physics and Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China
  b.School of Applied Chemistry and Engineering, University of Science and Technology of China, Hefei 230026, China
  c.Polymer Composite Engineering Laboratory, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China
  d.Huangpu Institute of Advanced Materials, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Guangzhou 510530, China
- Author bio：
  
  hongyinglv@ciac.ac.cn (H.Y.L.)
  xnyang@ciac.ac.cn (X.N.Y.)
- Funds：
  
  This work was financially supported by the National Natural Science Foundation of China (Nos. 21803069 and 21975249).
- DOI：10.1007/s10118-021-2639-3
  中图分类号：
- 收稿：2021-06-19，
  
  修回：2021-8-6，
  
  录用：2021-8-18，
  
  网络首发：2021-11-09，
  
  纸质出版：2022-01-01
- Accepted：
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Wu, H. Y.; Yang, L.; Tu, J. S.; Wang, J.; Li, J. G.; Lv, H. Y.; Yang, X. N. Hydrogels with dynamically controllable mechanics and biochemistry for 3D cell culture platforms. Chinese J. Polym. Sci. 2022, 40, 38–46

Hai-Yang Wu, Lei Yang, Jiang-Shan Tu, et al. Hydrogels with Dynamically Controllable Mechanics and Biochemistry for 3D Cell Culture Platforms[J]. Chinese Journal of Polymer Science, 2022, 40(1): 38-46.
Wu, H. Y.; Yang, L.; Tu, J. S.; Wang, J.; Li, J. G.; Lv, H. Y.; Yang, X. N. Hydrogels with dynamically controllable mechanics and biochemistry for 3D cell culture platforms. Chinese J. Polym. Sci. 2022, 40, 38–46 DOI： 10.1007/s10118-021-2639-3.

Hai-Yang Wu, Lei Yang, Jiang-Shan Tu, et al. Hydrogels with Dynamically Controllable Mechanics and Biochemistry for 3D Cell Culture Platforms[J]. Chinese Journal of Polymer Science, 2022, 40(1): 38-46. DOI： 10.1007/s10118-021-2639-3.

摘要

Abstract

Many cell-matrix interaction studies have proved that dynamic changes in the extracellular matrix (ECM) are crucial to maintain cellular properties and behaviors. Thus

developing materials that can recapitulate the dynamic attributes of the ECM is highly desired for three-dimensional (3D) cell culture platforms. To this end

we sought to develop a hydrogel system that would enable dynamic and reversible turning of its mechanical and biochemical properties

thus facilitating the control of cell culture to imitate the natural ECM. Herein

a hydrogel with dynamic mechanics and a biochemistry based on an addition-fragmentation chain transfer (AFCT) reaction was constructed. Thiol-modified hyaluronic acid (HA) and allyl sulfide-modified

-poly-L-lysine (EPL) were synthesized to form hydrogels

which were non-swellable and biocompatible. The reversible modulus of the hydrogel was first achieved through the AFCT reaction; the modulus can also be regulated stepwise by changing the dose of UVA irradiation. Dynamic patterning of fluorescent markers in the hydrogel was also realized. Therefore

this dynamically controllable hydrogel has great potential as a 3D cell culture platform for tissue engineering applications.

关键词

Keywords

references

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