Biodegradable Polypeptide-based Vesicles with Intrinsic Blue Fluorescence for Antibacterial Visualization

Yu-Ying Yang; Ling-Shan Chen; Min Sun; Cong-Yu Wang; Zhen Fan; Jian-Zhong Du

doi:10.1007/s10118-021-2593-0

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Biodegradable Polypeptide-based Vesicles with Intrinsic Blue Fluorescence for Antibacterial Visualization

ARTICLE | Updated：2021-06-18

- Biodegradable Polypeptide-based Vesicles with Intrinsic Blue Fluorescence for Antibacterial Visualization
- Biodegradable Polypeptide-based Vesicles with Intrinsic Blue Fluorescence for Antibacterial Visualization
- Chinese Journal of Polymer Science 2021年39卷第11期页码：1412-1420
- Affiliations：
  
  a.Department of Orthopedics, Shanghai Tenth People’s Hospital, Tongji University, Shanghai 200072, China
  b.Department of Polymeric Materials, School of Materials Science and Engineering, Tongji University, Shanghai 201804, China
  c.Institute for Advanced Study, Tongji University, Shanghai 200092, China
- Author bio：
  
  fanzhen2018@tongji.edu.cn (Z.F.)
  jzdu@tongji.edu.cn (J.Z.D.)
- Funds：
  
  This work was financially supported by the National Natural Science Foundation of China (Nos. 51803152, 22075212 and 21925505), the program for professor of special appointment (Eastern Scholar) at Shanghai institutions of higher learning and Natural Science Foundation of Shanghai (No. 19ZR1478800). J.D. is the recipient of National Science Fund for Distinguished Young Scholars.
- DOI：10.1007/s10118-021-2593-0
  中图分类号：
- 收稿：2021-02-27，
  
  修回：2021-4-14，
  
  录用：2021-4-16，
  
  网络首发：2021-06-18，
  
  纸质出版：2021-09-18
- Accepted：
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Yang, Y. Y.; Chen, L. S.; Sun, M.; Wang, C. Y.; Fan, Z.; Du, J. Z. Biodegradable polypeptide-based vesicles with intrinsic blue fluorescence for antibacterial visualization. Chinese J. Polym. Sci. 2021, 39, 1412–1420

Yu-Ying Yang, Ling-Shan Chen, Min Sun, et al. Biodegradable Polypeptide-based Vesicles with Intrinsic Blue Fluorescence for Antibacterial Visualization[J]. Chinese Journal of Polymer Science, 2021, 39(11): 1412-1420.
Yang, Y. Y.; Chen, L. S.; Sun, M.; Wang, C. Y.; Fan, Z.; Du, J. Z. Biodegradable polypeptide-based vesicles with intrinsic blue fluorescence for antibacterial visualization. Chinese J. Polym. Sci. 2021, 39, 1412–1420 DOI： 10.1007/s10118-021-2593-0.

Yu-Ying Yang, Ling-Shan Chen, Min Sun, et al. Biodegradable Polypeptide-based Vesicles with Intrinsic Blue Fluorescence for Antibacterial Visualization[J]. Chinese Journal of Polymer Science, 2021, 39(11): 1412-1420. DOI： 10.1007/s10118-021-2593-0.

摘要

Abstract

Fluorescence imaging has been an indispensable tool to provide dynamic information about the localization and quantity of organisms. Meanwhile

due to the intrinsic hollow structure and modularized biofunctionalities

polymer vesicles have been widely applied in biomedical field. However

most polymer vesicles are embedded with organic fluorophores for fluorescence imaging

which have certain drawbacks such as leakage and possible cytotoxicity. Here

we present a biodegradable polypeptide-based vesicle with intrinsic blue fluorescence without introducing any fluorophore for real-time visualization of antibacterial process. Through modular design to integrate multiple functional fragments

poly(

-caprolactone)-block-poly(tryptophan)-block-poly(lysine-

stat

-phenylalanine) [PCL

-PTrp

-P(Lys

stat

-Phe

)] was synthesized

where PCL chains form the hydrophobic membrane

P(Lys-

stat

-Phe) and PTrp provide intrinsic fluorescence and broad-spectrum antibacterial activity. It is noteworthy that the fluorescence emission was shifted from invisible ultraviolet range of amino acids to visible range (emission maximum at 436 nm)

which makes it possible to visualize the antibacterial process. In addition

through utilizing the intrinsic fluorescence of vesicles

confocal fluorescent imaging of vesicles with bacteria validated the specific adhesion of vesicle towards bacteria

and the bacterial death through membrane disruption. Overall

we provided a novel approach to developing biodegradable fluorescent polypeptide-based vesicles for real-time visualization of antibacterial process.

关键词

Keywords

references

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