Fabrication of Highly Anisotropic and Interconnected Porous Scaffolds to Promote Preosteoblast Proliferation for Bone Tissue Engineering

Ya-Hui Liu; Wei Liu; Zi-Li Zheng; Xin Wei; Nouman Ali Shah; Hao Lin; Bai-Song Zhao; Shi-Shu Huang; Jia-Zhuang Xu; Zhong-Ming Li

doi:10.1007/s10118-021-2573-4

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Fabrication of Highly Anisotropic and Interconnected Porous Scaffolds to Promote Preosteoblast Proliferation for Bone Tissue Engineering

ARTICLE | Updated：2021-05-25

- Fabrication of Highly Anisotropic and Interconnected Porous Scaffolds to Promote Preosteoblast Proliferation for Bone Tissue Engineering
- Fabrication of Highly Anisotropic and Interconnected Porous Scaffolds to Promote Preosteoblast Proliferation for Bone Tissue Engineering
- Chinese Journal of Polymer Science 2021年39卷第9期页码：1191-1199
- Affiliations：
  
  a.College of Polymer Science and Engineering, State Key Laboratory of Polymer Materials Engineering, Sichuan University, Chengdu 610065, China
  b.Department of Anesthesiology, Guangzhou Women and Children's Medical Center, Guangzhou 510623, China
  c.Department of Orthopaedic Surgery, West China Hospital, Sichuan University, Chengdu 610065, China
- Author bio：
  
  zhaobaisong@gwcmc.org (B.S.Z.)
  jzxu@scu.edu.cn (J.Z.X.)
- Funds：
  
  The authors gratefully acknowledge the financial support from the National Key R&D Program of China (No. 2018YFB0704200) and the National Natural Science Foundation of China (Nos. 51803139, 52022061, 52033005 and 52003169).
- DOI：10.1007/s10118-021-2573-4
  中图分类号：
- 收稿：2021-01-30，
  
  修回：2021-2-22，
  
  录用：2021-2-24，
  
  网络首发：2021-05-25，
  
  纸质出版：2021-07-18
- Accepted：
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Liu, Y. H.; Liu, W.; Zheng, Z. L.; Wei, X.; Shah, N. A.; Lin, H.; Zhao, B. S.; Huang, S. S.; Xu, J. Z.; Li, Z. M. Fabrication of highly anisotropic and interconnected porous scaffolds to promote preosteoblast proliferation for bone tissue engineering. Chinese J. Polym. Sci. 2021, 39, 1191–1199

Ya-Hui Liu, Wei Liu, Zi-Li Zheng, et al. Fabrication of Highly Anisotropic and Interconnected Porous Scaffolds to Promote Preosteoblast Proliferation for Bone Tissue Engineering[J]. Chinese Journal of Polymer Science, 2021, 39(9): 1191-1199.
Liu, Y. H.; Liu, W.; Zheng, Z. L.; Wei, X.; Shah, N. A.; Lin, H.; Zhao, B. S.; Huang, S. S.; Xu, J. Z.; Li, Z. M. Fabrication of highly anisotropic and interconnected porous scaffolds to promote preosteoblast proliferation for bone tissue engineering. Chinese J. Polym. Sci. 2021, 39, 1191–1199 DOI： 10.1007/s10118-021-2573-4.

Ya-Hui Liu, Wei Liu, Zi-Li Zheng, et al. Fabrication of Highly Anisotropic and Interconnected Porous Scaffolds to Promote Preosteoblast Proliferation for Bone Tissue Engineering[J]. Chinese Journal of Polymer Science, 2021, 39(9): 1191-1199. DOI： 10.1007/s10118-021-2573-4.

摘要

Abstract

Mimicking the complex structure of natural bone remains a challenge for bone tissue scaffolds. In this study

a novel processing strategy was developed to prepare the bone-like scaffolds that are featured by highly oriented and fully interconnected pores. This type of biomimetic scaffolds was evolved from solid phase stretching of immiscible polycaprolactone (PCL)/poly(ethylene oxide) (PEO) blends with co-continuous structure and the pore morphology was inherited from selective extraction of water soluble PEO phase. The pore anisotropy was readily tuned by varying the stretching strain without loss of interconnectivity. Significant promotion in preosteoblast proliferation

alkaline phosphatase activity and osteogenic gene expression was observed in the oriented porous scaffolds compared to the isotropic porous counterpart. The oriented architecture provided a topographical cue for aligned growth of preosteoblasts

which activated the Wnt/

-catenin signaling pathway. The proposed strategy enriches the toolbox for the scaffold design and fabrication for bone tissue engineering.

关键词

Keywords

references

Cui, L.; Zhang, J.; Zou, J.; Yang, X.; Guo, H.; Tian, H.; Zhang, P.; Wang, Y.; Zhang, N.; Zhuang, X.; Li, Z.; Ding, J.; Chen, X. . Electroactive composite scaffold with locally expressed osteoinductive factor for synergistic bone repair upon electrical stimulation . Biomaterials , 2020 . 230 119617 DOI: 10.1016/j.biomaterials.2019.119617 http://doi.org/10.1016/j.biomaterials.2019.119617 .

Hutmacher, D. W. . Scaffolds in tissue engineering bone and cartilage . Biomaterials , 2000 . 21 2529 - 2543 . DOI: 10.1016/S0142-9612(00)00121-6 http://doi.org/10.1016/S0142-9612(00)00121-6 .

Slotkin, J. R.; Pritchard, C. D.; Luque, B.; Ye, J.; Layer, R. T.; Lawrence, M. S.; O'Shea, T. M.; Roy, R. R.; Zhong, H.; Vollenweider, I.; Edgerton, V. R.; Courtine, G.; Woodard, E. J.; Langer, R. . Biodegradable scaffolds promote tissue remodeling and functional improvement in non-human primates with acute spinal cord injury . Biomaterials , 2017 . 123 63 - 76 . DOI: 10.1016/j.biomaterials.2017.01.024 http://doi.org/10.1016/j.biomaterials.2017.01.024 .

Zhu, T.; Cui, Y.; Zhang, M.; Zhao, D.; Liu, G.; Ding, J. . Engineered three-dimensional scaffolds for enhanced bone regeneration in osteonecrosis . Bioact. Mater. , 2020 . 5 584 - 601 . DOI: 10.1016/j.bioactmat.2020.04.008 http://doi.org/10.1016/j.bioactmat.2020.04.008 .

Ghobeira, R.; Philips, C.; Liefooghe, L.; Verdonck, M.; Asadian, M.; Cools, P.; Declercq, H.; de Vos, W. H.; de Geyter, N.; Morent, R. . Synergetic effect of electrospun PCL fiber size, orientation and plasma-modified surface chemistry on stem cell behavior . Appl. Surf. Sci. , 2019 . 485 204 - 221 . DOI: 10.1016/j.apsusc.2019.04.109 http://doi.org/10.1016/j.apsusc.2019.04.109 .

Li, X; Yin, H. M.; Luo, E.; Zhu, S. S.; Wang, P.; Zhang, Z.; Liao, G. Q.; Xu, J. Z.; Li, Z. M.; Li, J. H. . Accelerating bone healing by decorating BMP-2 on porous composite scaffolds . ACS Appl. Bio Mater. , 2019 . 2 5717 - 5726 . DOI: 10.1021/acsabm.9b00761 http://doi.org/10.1021/acsabm.9b00761 .

Zeng, W.; Hu, W. K.; Li, H.; Jing, Y. H.; Kang, H.; Jiang, Q.; Zhang, C. . Preparation and characterization of poly( γ -glutamic acid) hydrogels as potential tissue engineering scaffolds . Chinese J. Polym. Sci. , 2014 . 32 1507 - 1514 . DOI: 10.1007/s10118-014-1536-4 http://doi.org/10.1007/s10118-014-1536-4 .

Jia, S.; Wang, J.; Zhang, T.; Pan, W.; Li, Z.; He, X.; Yang, C.; Wu, Q.; Sun, W.; Xiong, Z.; Hao, D. . Multilayered scaffold with a compact interfacial layer enhances osteochondral defect repair . ACS Appl. Mater. Interfaces , 2018 . 10 20296 - 20305 . DOI: 10.1021/acsami.8b03445 http://doi.org/10.1021/acsami.8b03445 .

Scotti, K. L.; Dunand, D. C. . Freeze casting–a review of processing, microstructure and properties via the open data repository, FreezeCasting.net . Prog. Mater Sci. , 2018 . 94 243 - 305 . DOI: 10.1016/j.pmatsci.2018.01.001 http://doi.org/10.1016/j.pmatsci.2018.01.001 .

Wang, P.; Yin, H.M.; Li, X.; Liu, W.; Chu, Y.X; Wang, Y.; Wang, Y.; Xu, J.Z.; Li, Z.M.; Li, J.H. . Simultaneously constructing nanotopographical and chemical cues in 3D-printed polylactic acid scaffolds to promote bone regeneration . Mat. Sci. Eng. C-Mater. , 2021 . 118 111457 DOI: 10.1016/j.msec.2020.111457 http://doi.org/10.1016/j.msec.2020.111457 .

Deville, S.; Saiz, E., Tomsia, A. P. . Freeze casting of hydroxyapatite scaffolds for bone tissue engineering . Biomaterials , 2006 . 27 5480 - 5489 . DOI: 10.1016/j.biomaterials.2006.06.028 http://doi.org/10.1016/j.biomaterials.2006.06.028 .

Huang, L.; Huang, J.; Shao, H.; Hu, X.; Cao, C.; Fan, S.; Song, L.; Zhang, Y. . Silk scaffolds with gradient pore structure and improved cell infiltration performance . Mater. Sci. Eng. C Mater. Biol. Appl. , 2019 . 94 179 - 189 . DOI: 10.1016/j.msec.2018.09.034 http://doi.org/10.1016/j.msec.2018.09.034 .

Cai, X.; Chen, L.; Jiang, T.; Shen, X.; Hu, J.; Tong, H. . Facile synthesis of anisotropic porous chitosan/hydroxyapatite scaffolds for bone tissue engineering . J. Mater. Chem. , 2011 . 21 12015 DOI: 10.1039/c1jm11503k http://doi.org/10.1039/c1jm11503k .

Ran, J.; Xie, L.; Sun, G.; Hu, J.; Chen, S.; Jiang, P.; Shen, X.; Tong, H. . A facile method for the preparation of chitosan-based scaffolds with anisotropic pores for tissue engineering applications . Carbohydr. Polym. , 2016 . 152 615 - 623 . DOI: 10.1016/j.carbpol.2016.07.054 http://doi.org/10.1016/j.carbpol.2016.07.054 .

Afshar, M.; Anaraki, A. P.; Montazerian, H.; Kadkhodapour, J. . Additive manufacturing and mechanical characterization of graded porosity scaffolds designed based on triply periodic minimal surface architectures . J. Mech. Behav. Biomed. Mater. , 2016 . 62 481 - 494 . DOI: 10.1016/j.jmbbm.2016.05.027 http://doi.org/10.1016/j.jmbbm.2016.05.027 .

Sultan, S.; Mathew, A. P. . 3D printed scaffolds with gradient porosity based on a cellulose nanocrystal hydrogel . Nanoscale , 2018 . 10 4421 - 4431 . DOI: 10.1039/C7NR08966J http://doi.org/10.1039/C7NR08966J .

Zhang, H.; Hussain, I.; Brust, M.; Butler, M. F.; Rannard, S. P.; Cooper, A. I. . Aligned two- and three-dimensional structures by directional freezing of polymers and nanoparticles . Nat. Mater. , 2005 . 4 787 - 793 . DOI: 10.1038/nmat1487 http://doi.org/10.1038/nmat1487 .

Fu, J.; Liu, X.; Tan, L.; Cui, Z.; Liang, Y.; Li, Z.; Zhu, S.; Zheng, Y.; Kwok Yeung, K. W.; Chu, P. K.; Wu, S. . Modulation of the mechanosensing of mesenchymal stem cells by laser-induced patterning for the acceleration of tissue reconstruction through the Wnt/ β -catenin signaling pathway activation . Acta Biomater. , 2020 . 101 152 - 167 . DOI: 10.1016/j.actbio.2019.10.041 http://doi.org/10.1016/j.actbio.2019.10.041 .

Nakayama, M.; Lim, W. Q.; Kajiyama, S.; Kumamoto, A.; Ikuhara, Y.; Kato, T.; Zhao, Y. . Liquid-crystalline hydroxyapatite/polymer nanorod hybrids: potential bioplatform for photodynamic therapy and cellular scaffolds . ACS Appl. Mater. Interfaces , 2019 . 11 17759 - 17765 . DOI: 10.1021/acsami.9b02485 http://doi.org/10.1021/acsami.9b02485 .

Xiao, Q. R.; Zhang, N.; Wang, X.; Man, X. Y.; Yang, K.; Lü, L. X.; Huang, N. P. . Oriented surface nanotopography promotes the osteogenesis of mesenchymal stem cells . Adv. Mater. Imterface , 2017 . 4 1600652 DOI: 10.1002/admi.201600652 http://doi.org/10.1002/admi.201600652 .

Zhang, K. K.; Wang, Y. Q.; Jiang, J.; Wang, X. F.; Hou, J. H.; Sun, S. H.; Li, Q. . Fabrication of highly interconnected porous poly(-caprolactone) scaffolds with supercritical CO 2 foaming and polymer leaching . J. Mater. Sci. , 2019 . 54 5112 - 5126 . DOI: 10.1007/s10853-018-3166-7 http://doi.org/10.1007/s10853-018-3166-7 .

Sun, Z. B.; Wei, Q. Y.; Xie, X. L.; Xu, L.; Ji, X.; Zhou, L.; Zhong G. J.; Li, Z. M. . Spatial dependence of ordering process in bulk materials of polylactide and its multiple system during hygrothermal aging . Polym. Degrad. Stabil. , 2020 . 174 109107 DOI: 10.1016/j.polymdegradstab.2020.109107 http://doi.org/10.1016/j.polymdegradstab.2020.109107 .

Guarino, V.; Guaccio, A.; Ambrosio, L. . Manipulating co-continuous polymer blends to create PCL scaffolds with fully interconnected and anisotropic pore architecture . J. Appl. Biomater. Biomech. , 2011 . 9 34 - 39 . DOI: 10.5301/JABB.2011.6473 http://doi.org/10.5301/JABB.2011.6473 .

Yuan, Z.; Favis, B. D. . Coarsening of immiscible co-continuous blends during quiescent annealing . AICHE J. , 2005 . 51 271 - 280 . DOI: 10.1002/aic.10281 http://doi.org/10.1002/aic.10281 .

Su, B.; Zhao, Y. S.; Chen, F.; Fu, Q. . Effect of microdomain structure on the mechanical behavior of binary blends . Chinese J. Polym. Sci. , 2015 . 33 964 - 975 . DOI: 10.1007/s10118-015-1649-4 http://doi.org/10.1007/s10118-015-1649-4 .

Zhao, D.; Zhu, T.; Li, J.; Cui, L.; Zhang, Z.; Zhuang, X.; Ding, J. . Poly(lactic- co -glycolic acid)-based composite bone-substitute materials . Bioact. Mater. , 2021 . 6 346 - 360 . DOI: 10.1016/j.bioactmat.2020.08.016 http://doi.org/10.1016/j.bioactmat.2020.08.016 .

Ghosh, S.; Vianac, J. C.; Reis, R. L.; Mano, J. F. . Development of porous lamellar poly(L-lactic acid) scaffolds by conventional injection molding process . Acta Biomater. , 2008 . 4 887 - 896 . DOI: 10.1016/j.actbio.2008.03.001 http://doi.org/10.1016/j.actbio.2008.03.001 .

Liu, R. H.; Jin, L. N.; Lu, D.; Hu, G.; He, L.; Shan, J. . Bi-continuous phases structured nanocomposite polymer electrolytes: facile preparation and electrochemical properties . Electrochim. Acta , 2013 . 114 372 - 378 . DOI: 10.1016/j.electacta.2013.09.166 http://doi.org/10.1016/j.electacta.2013.09.166 .

Xue, L.; Zhang, J.; Han, Y. . Phase separation induced ordered patterns in thin polymer blend films . Prog. Polym. Sci. , 2012 . 37 564 - 594 . DOI: 10.1016/j.progpolymsci.2011.09.001 http://doi.org/10.1016/j.progpolymsci.2011.09.001 .

Yin, H. M.; Huang, Y. F.; Ren, Y.; Wang, P.; Zhao, B.; Li, J. H.; Xu, J. Z.; Li, Z. M. . Toward biomimetic porous poly( ε -caprolactone) scaffolds: structural evolution and morphological control during solid phase extrusion . Composites Sci. Technol. , 2018 . 156 192 - 202 . DOI: 10.1016/j.compscitech.2018.01.009 http://doi.org/10.1016/j.compscitech.2018.01.009 .

Yin, H. M.; Li, X.; Xu, J. Z.; Zhao, B.; Li, J. H.; Li, Z. M. . Highly aligned and interconnected porous poly( ε -caprolactone) scaffolds derived from co-continuous polymer blends . Materials & Design , 2017 . 128 112 - 118 . .

Wei, Y.; Huang, R.; Dong, P.; Qi, X.D.; Fu, Q. . Preparation of polylactide/poly(ether)urethane blends with excellent electro-actuated shape memory via incorporating carbon black and carbon nanotubes hybrids fillers . Chinese J. Polym. Sci. , 2018 . 36 1175 - 1186 . DOI: 10.1007/s10118-018-2138-3 http://doi.org/10.1007/s10118-018-2138-3 .

Ghavidel Mehr, N.; Li, X.; Ariganello, M. B.; Hoemann, C. D.; Favis, B. D. . Poly( ε -caprolactone) scaffolds of highly controlled porosity and interconnectivity derived from co-continuous polymer blends: model bead and cell infiltration behavior . J. Mater. Sci. Mater. Med. , 2014 . 25 2083 - 2093 . DOI: 10.1007/s10856-014-5256-7 http://doi.org/10.1007/s10856-014-5256-7 .

Nofar, M.; Sacligil, D.; Carreau, P. J.; Kamal, M. R.; Heuzey, M. C. . Poly(lactic acid) blends: processing, properties and applications . Int. J. Biol. Macromol. , 2019 . 125 307 - 360 . DOI: 10.1016/j.ijbiomac.2018.12.002 http://doi.org/10.1016/j.ijbiomac.2018.12.002 .

Jašo, V.; Cvetinov, M.; Rakić, S.; Petrović, Z. S. . Bio-plastics and elastomers from polylactic acid/thermoplastic polyurethane blends . J. Appl. Polym. Sci. , 2014 . 131 41104 DOI: 10.1002/app.41104 http://doi.org/10.1002/app.41104 .

Yin, H. M.; Liu, W.; Huang, Y. F.; Ren, Y.; Xu, L.; Xu, J. Z.; Zhao, B. S.; Li, Z. M. . Surface epitaxial crystallization-directed nanotopography for accelerating preosteoblast proliferation and osteogenic differentiation . ACS Appl. Mater. Interfaces , 2019 . 11 42956 - 42963 . DOI: 10.1021/acsami.9b14800 http://doi.org/10.1021/acsami.9b14800 .

Danilevicius, P.; Georgiadi, L.; Pateman, C. J.; Claeyssens, F.; Chatzinikolaidou, M.; Farsari, M. . The effect of porosity on cell ingrowth into accurately defined, laser-made, polylactide-based 3D scaffolds . Appl. Surf. Sci. , 2015 . 336 2 - 10 . DOI: 10.1016/j.apsusc.2014.06.012 http://doi.org/10.1016/j.apsusc.2014.06.012 .

Jegat, C.; Virgilio, N., Favis, B. D. . Self-assembly of oil microdroplets at the interface in co-continuous polymer blends . Eur. Polym. J. , 2017 . 93 259 - 271 . DOI: 10.1016/j.eurpolymj.2017.06.002 http://doi.org/10.1016/j.eurpolymj.2017.06.002 .

Reignier, J.; Huneault, M. A. . Preparation of interconnected poly( ε -caprolactone) porous scaffolds by a combination of polymer and salt particulate leaching . Polymer , 2006 . 47 4703 - 4717 . DOI: 10.1016/j.polymer.2006.04.029 http://doi.org/10.1016/j.polymer.2006.04.029 .

Kamal, T.; Shin, T. J.; Park, S. Y. . Uniaxial tensile deformation of poly( ε -caprolactone) studied with SAXS and WAXS techniques using synchrotron radiation . Macromolecules , 2012 . 45 8752 - 8759 . DOI: 10.1021/ma301714f http://doi.org/10.1021/ma301714f .

Wang, L.; Qiu, Y.; Guo, Y.; Si, Y.; Liu, L.; Cao, J.; Yu, J.; Li, X.; Zhang, Q.; Ding, B. . Smart, elastic, and nanofiber-based 3D scaffolds with self-deploying capability for osteoporotic bone regeneration . Nano Lett. , 2019 . 19 9112 - 9120 . DOI: 10.1021/acs.nanolett.9b04313 http://doi.org/10.1021/acs.nanolett.9b04313 .

Lei, M.; Qu, X.; Liu, H.; Liu, Y.; Wang, S.; Wu, S.; Bentley, W. E.; Payne, G. F.; Liu, C. . Programmable electrofabrication of porous Janus films with tunable janus balance for anisotropic cell guidance and tissue regeneration . Adv. Fun. Mater. , 2019 . 29 1900065 DOI: 10.1002/adfm.201900065 http://doi.org/10.1002/adfm.201900065 .

Baron, R.; Kneissel, M. . WNT signaling in bone homeostasis and disease: from human mutations to treatments . Nat. Med. , 2013 . 19 179 - 192 . DOI: 10.1038/nm.3074 http://doi.org/10.1038/nm.3074 .

Zhao, X.; Han, Y.; Zhu, T.; Feng, N.; Sun, Y.; Song, Z.; Li, S.; Liu, J.; Ding, J. . Electrospun polylactide-nano-hydroxyapatite-vancomycin composite scaffolds for advanced osteomyelitis therapy . J. Biomed. Nanotechnol. , 2019 . 15 1213 - 1222 . DOI: 10.1166/jbn.2019.2773 http://doi.org/10.1166/jbn.2019.2773 .

Yang, Y.; Yu, M. R.; Boke, F.; Qin, Q.; Hubner, R.; Knust, S.; Schwiderek, S.; Grundmeier, G.; Fischer, H.; Keller, A. . Effect of nanoscale surface topography on the adsorption of globular proteins . Appl. Surf. Sci. , 2021 . 535 147671 DOI: 10.1016/j.apsusc.2020.147671 http://doi.org/10.1016/j.apsusc.2020.147671 .

Metavarayuth, K.; Sitasuwan, P.; Zhao, X.; Lin, Y.; Wang, Q. . Influence of surface topographical cues on the differentiation of mesenchymal stem cells in vitro . ACS Biomater. Sci. Eng , 2016 . 2 142 - 151 . DOI: 10.1021/acsbiomaterials.5b00377 http://doi.org/10.1021/acsbiomaterials.5b00377 .

Yao, X.; Peng, R.; Ding, J. . Cell-material interactions revealed via material techniques of surface patterning . Adv. Mater. , 2013 . 25 5257 - 5286 . DOI: 10.1002/adma.201301762 http://doi.org/10.1002/adma.201301762 .

Lee, M. S.; Lee, D. H.; Jeon, J.; Oh, S. H.; Yang, H. S. . Topographically defined, biodegradable nanopatterned patches to regulate cell fate and acceleration of bone regeneration . ACS Appl. Mater. Interfaces , 2018 . 10 38780 - 38790 . DOI: 10.1021/acsami.8b14745 http://doi.org/10.1021/acsami.8b14745 .

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