Preparation and Characterization of Attractive Poly(amino acid) Hydrogels Based on 2-Ureido-4[1<italic style="font-style: italic">H</italic>]-pyrimidinone

Zhen Shi; Qi Wang; Gui-Fei Li; Yu-Feng Shou; Hong-Jie Zong; Shi-Feng Yan; Kun-Xi Zhang; Jing-Bo Yin

doi:10.1007/s10118-021-2498-y

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Preparation and Characterization of Attractive Poly(amino acid) Hydrogels Based on 2-Ureido-4[1H]-pyrimidinone

ARTICLE | Updated：2020-10-15

- Preparation and Characterization of Attractive Poly(amino acid) Hydrogels Based on 2-Ureido-4[1H]-pyrimidinone
- Preparation and Characterization of Attractive Poly(amino acid) Hydrogels Based on 2-Ureido-4[1H]-pyrimidinone
- Chinese Journal of Polymer Science 2021年39卷第3期页码：327-336
- Affiliations：
  
  Department of Polymer Materials, School of Materials Science and Engineering, Shanghai University, Shanghai 200444, China
- Author bio：
  
  guifeili@shu.edu.cn (G.F.L.)
  jbyin@oa.shu.edu.cn (J.B.Y.)
- Funds：
  
  This work was financially supported by the National Natural Science Foundation of China (No. 51773113). The authors would also like to thank Mr Qiang Li of the instrumental analysis research center of Shanghai University for his help in 1H-NMR.
- DOI：10.1007/s10118-021-2498-y
  中图分类号：
- 收稿：2020-07-14，
  
  修回：2020-8-28，
  
  录用：2020-8-31，
  
  网络首发：2020-10-15，
  
  纸质出版：2021-01-02
- Accepted：
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Shi, Z.; Wang, Q.; Li, G. F.; Shou, Y. F.; Zong, H. J.; Yan, S. F.; Zhang, K. X.; Yin, J. B. Preparation and characterization of attractive poly(amino acid) hydrogels based on 2-ureido-4[1, H,]-pyrimidinone. Chinese J. Polym. Sci. 2021, 39, 327–336

Zhen Shi, Qi Wang, Gui-Fei Li, et al. Preparation and Characterization of Attractive Poly(amino acid) Hydrogels Based on 2-Ureido-4[1H]-pyrimidinone[J]. Chinese Journal of Polymer Science, 2021, 39(3): 327-336. DOI: 10.1007/s10118-021-2498-y.
Shi, Z.; Wang, Q.; Li, G. F.; Shou, Y. F.; Zong, H. J.; Yan, S. F.; Zhang, K. X.; Yin, J. B. Preparation and characterization of attractive poly(amino acid) hydrogels based on 2-ureido-4[1, H,]-pyrimidinone. Chinese J. Polym. Sci. 2021, 39, 327–336 DOI： 10.1007/s10118-021-2498-y.

Zhen Shi, Qi Wang, Gui-Fei Li, et al. Preparation and Characterization of Attractive Poly(amino acid) Hydrogels Based on 2-Ureido-4[1H]-pyrimidinone[J]. Chinese Journal of Polymer Science, 2021, 39(3): 327-336. DOI: 10.1007/s10118-021-2498-y. DOI：

摘要

Abstract

Self-healing hydrogels with the shear-thinning property are novel injectable materials and are superior to traditional injectable hydrogels. The self-healing hydrogels based on 2-ureido-4[1

]-pyrimidinone (UPy) have recently received extensive attention due to their dynamic reversibility of UPy dimerization. However

generally

UPy-based self-healing hydrogels exhibit poor stability

cannot degrade

in vivo

and can hardly be excreted from the body

which considerably limit their bio-application. Here

using poly(

-glutamic acid) (PLGA) as biodegradable matrix

branching

-hydroxy-

-amino poly(ethylene oxide) (HAPEO) as bridging molecule to introduce UPy

and ethyl acrylate polyethylene glycol (MAPEG) to introduce double bond

the hydrogel precursors (PMHU) are prepared. A library of the self-healing hydrogels has been achieved with well self-healable and shear-thinning properties. With the increase of MAPEG grafting ratio

the storage modulus of the self-healing hydrogels decreases. The self-healing hydrogels are stable in solution only for 6 h

hard to meet the requirements of tissue regeneration. Consequently

ultraviolet (UV) photo-crosslinking is involved to obtain the dual crosslinking hydrogels with enhanced mechanical properties and stability. When MAPEG grafting ratio is 35.5%

the dual crosslinking hydrogels can maintain the shape in phosphate-buffered saline solution (PBS) for at least 8 days. Loading with adipose-derived stem cell spheroids

the self-healing hydrogels are injected and self-heal to a whole

and then they are crosslinked

in situ via

UV-irradiation

obtaining the dual crosslinking hydrogels/cell spheroids complex with cell viability of 86.7%±6.0%

which demonstrates excellent injectability

subcutaneous gelatinization

and biocompatibility of hydrogels as cell carriers. The novel PMHU hydrogels crosslinked by quadruple hydrogen bonding and then dual photo-crosslinking of double bond are expected to be applied for minimal invasive surgery or therapies in tissue engineering.

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references

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Preparation and Characterization of Attractive Poly(amino acid) Hydrogels Based on 2-Ureido-4[1H]-pyrimidinone

DOI：10.1007/s10118-021-2498-y

摘要

Abstract

关键词

Keywords

references