Thermoresponsive Nanogels from Dendronized Copolymers for Complexation, Protection and Release of Nucleic Acids

Yi Yao; Jian-Hui Wu; Shi-Jie Cao; Bi-Yi Xu; Jia-Tao Yan; Di Wu; Wen Li; Afang Zhang

doi:10.1007/s10118-020-2452-4

Your Location：

Home >

Browse articles >

Thermoresponsive Nanogels from Dendronized Copolymers for Complexation, Protection and Release of Nucleic Acids

ARTICLE | Updated：2020-07-07

- Thermoresponsive Nanogels from Dendronized Copolymers for Complexation, Protection and Release of Nucleic Acids
- Thermoresponsive Nanogels from Dendronized Copolymers for Complexation, Protection and Release of Nucleic Acids
- Chinese Journal of Polymer Science 2020年38卷第11期页码：1164-1170
- Affiliations：
  
  Department of Polymer Materials, School of Materials Science & Engineering, Shanghai University, Shanghai 200444, China
- Author bio：
  
  wli@shu.edu.cn (W.L.)
  azhang@shu.edu.cn (A.Z.)
- Funds：
  
  We sincerely thank Dr. Hongmei Deng from the Instrumental Analysis and Research Center of Shanghai University for her assistance with NMR measurements. This work was financially supported by the National Natural Science Foundation of China (Nos. 21971161, 21971160, and 21574078), Shanghai Pujiang Program (No. 19PJ1403700), and Program for Professor of Special Appointment (Eastern Scholar) at Shanghai Institutions of Higher Learning.
- DOI：10.1007/s10118-020-2452-4
  中图分类号：
- 收稿：2020-03-03，
  
  修回：2020-4-4，
  
  录用：2020-5-6，
  
  网络首发：2020-07-07，
  
  纸质出版：2020-08-14
- Accepted：
Scan QR Code
Yao, Y.; Wu, J. H.; Cao, S. J.; Xu, B. Y.; Yan, J. T.; Wu, D.; Li, W.; Zhang, A. Thermoresponsive nanogels from dendronized copolymers for complexation, protection and release of nucleic acids. Chinese J. Polym. Sci. 2020, 38, 1164–1170

Yi Yao, Jian-Hui Wu, Shi-Jie Cao, et al. Thermoresponsive Nanogels from Dendronized Copolymers for Complexation, Protection and Release of Nucleic Acids[J]. Chinese Journal of Polymer Science, 2020, 38(11): 1164-1170.
Yao, Y.; Wu, J. H.; Cao, S. J.; Xu, B. Y.; Yan, J. T.; Wu, D.; Li, W.; Zhang, A. Thermoresponsive nanogels from dendronized copolymers for complexation, protection and release of nucleic acids. Chinese J. Polym. Sci. 2020, 38, 1164–1170 DOI： 10.1007/s10118-020-2452-4.

Yi Yao, Jian-Hui Wu, Shi-Jie Cao, et al. Thermoresponsive Nanogels from Dendronized Copolymers for Complexation, Protection and Release of Nucleic Acids[J]. Chinese Journal of Polymer Science, 2020, 38(11): 1164-1170. DOI： 10.1007/s10118-020-2452-4.

摘要

Abstract

A series of thermoresponsive cationic dendronized copolymers and their corresponding nanogels containing dendritic oligoethylene glycol (OEG) units and guanidine groups were prepared

and their complexation

protection

and release of nucleic acids were investigated. The dendritic OEGs endow these copolymer materials with good biocompatibility and characteristic thermoresponsiveness

while cationic guanidine groups can efficiently bind with the nucleic acids. The dendritic topology also affords the copolymers specific shielding effect which plays an essential role in protecting the activity of nucleic acids. At room temperature

dendronized copolymers and the corresponding nanogels could efficiently capture and condense the nucleic acids

while above their cloud points (

more than 75% of siRNA could be released in 1 h triggered by ATP. More importantly

the copolymer showed protective capability to siRNA

while nanogels exhibit even better protection when compared to the copolymers due to the synergetic effect from the three-dimensional cross-linked network and high density of dendritic units in vicinity. This kind of smart dendronized copolymer nanogels form a novel class of scaffolds as promising materials for biomedical applications.

关键词

Keywords

references

Zan, G.; Wu, Q. . Biomimetic and bioinspired synthesis of nanomaterials/nanostructures . Adv. Mater. , 2016 . 28 2099 - 2147 . DOI: 10.1002/adma.201503215 http://doi.org/10.1002/adma.201503215 .

Trzebicka, B.; Szweda, R.; Kosowski, D.; Szweda, D.; Otulakowski, Ł.; Haladjova, E.; Dworak, A. . Thermoresponsive polymer-peptide/protein conjugates . Prog. Polym. Sci. , 2017 . 68 35 - 76 . DOI: 10.1016/j.progpolymsci.2016.12.004 http://doi.org/10.1016/j.progpolymsci.2016.12.004 .

Wu, Y.; Ng, D. Y.; Kuan, S. L.; Weil, T. . Protein-polymer therapeutics: a macromolecular perspective . Biomater. Sci. , 2015 . 3 214 - 230 . DOI: 10.1039/C4BM00270A http://doi.org/10.1039/C4BM00270A .

Zhu, J.; Zhang, Y.; Lu, D.; Zare, R. N.; Ge, J.; Liu, Z. . Temperature-responsive enzyme-polymer nanoconjugates with enhanced catalytic activities in organic media . Chem. Commun. , 2013 . 49 6090 - 6092 . DOI: 10.1039/c3cc42493f http://doi.org/10.1039/c3cc42493f .

Wang, B.; Ma, R.; Liu, G.; Li, Y.; Liu, X.; An, Y.; Shi, L. . Glucose-responsive micelles from self-assembly of poly(ethylene glycol)- b -poly(acrylic acid- co -acrylamidophenylboronic acid) and the controlled release of insulin . Langmuir , 2009 . 25 12522 - 12528 . DOI: 10.1021/la901776a http://doi.org/10.1021/la901776a .

Liu, J.; Pang, Y.; Huang, W.; Zhu, Z.; Zhu, X.; Zhou, Y.; Yan, D. . Redox-responsive polyphosphate nanosized assemblies: a smart drug delivery platform for cancer therapy . Biomacromolecules , 2011 . 12 2407 - 2415 . DOI: 10.1021/bm2005164 http://doi.org/10.1021/bm2005164 .

Mogaki, R.; Hashim, P. K.; Okuro, K.; Aida, T. . Guanidinium-based "molecular glues" for modulation of biomolecular functions . Chem. Soc. Rev. , 2017 . 46 6480 - 6491 . DOI: 10.1039/C7CS00647K http://doi.org/10.1039/C7CS00647K .

Yu, S.; Chen, J.; Dong, R.; Su, Y.; Ji, B.; Zhou, Y.; Zhu, X.; Yan, D. . Enhanced gene transfection efficiency of PDMAEMA by incorporating hydrophobic hyperbranched polymer cores: effect of degree of branching . Polym. Chem. , 2012 . 3 3324 - 3329 . DOI: 10.1039/c2py20487h http://doi.org/10.1039/c2py20487h .

Yang, J.; Zhang, Q.; Chang, H.; Cheng, Y. . Surface-engineered dendrimers in gene delivery . Chem. Rev. , 2015 . 115 5274 - 300 . DOI: 10.1021/cr500542t http://doi.org/10.1021/cr500542t .

Feng, C.; Huang, X. . Polymer brushes: efficient synthesis and applications . Acc. Chem. Res. , 2018 . 51 2314 - 2323 . DOI: 10.1021/acs.accounts.8b00307 http://doi.org/10.1021/acs.accounts.8b00307 .

Huang, X.; Yin, Y.; Tang, Y.; Bai, X.; Zhang, Z.; Xu, J.; Liu, J.; Shen, J. . Smart microgel catalyst with modulatory glutathione peroxidase activity . Soft Matter , 2009 . 5 1905 - 1911 . DOI: 10.1039/b816888a http://doi.org/10.1039/b816888a .

Liu, F.; Cui, Y.; Wang, L.; Wang, H.; Yuan, Y.; Pan, J.; Chen, H.; Yuan, L. . Temperature-responsive poly( N -isopropylacrylamide) modified gold nanoparticle-protein conjugates for bioactivity modulation . ACS Appl. Mater. Interfaces , 2015 . 7 11547 - 11554 . DOI: 10.1021/acsami.5b02502 http://doi.org/10.1021/acsami.5b02502 .

Li, F.; Gao, Q.; Yang, M.; Guo, W. . Regulation of catalytic DNA activities with thermosensitive gold nanoparticle surfaces . Langmuir , 2018 . 34 14932 - 14939 . DOI: 10.1021/acs.langmuir.8b02149 http://doi.org/10.1021/acs.langmuir.8b02149 .

Yoshimatsu, K.; Lesel, B. K.; Yonamine, Y.; Beierle, J. M.; Hoshino, Y.; Shea, K. J. . Temperature-responsive “catch and release” of proteins by using multifunctional polymer-based nanoparticles . Angew. Chem. Int. Ed. , 2012 . 51 2405 - 2408 . DOI: 10.1002/anie.201107797 http://doi.org/10.1002/anie.201107797 .

Raynal, M.; Ballester, P.; Vidal-Ferran, A.; van Leeuwen, P. W. . Supramolecular catalysis. Part 2: artificial enzyme mimics . Chem. Soc. Rev. , 2014 . 43 1734 - 1787 . DOI: 10.1039/C3CS60037H http://doi.org/10.1039/C3CS60037H .

Shi, H.; Liu, Y.; Qu, R.; Li, Y.; Ma, R.; An, Y.; Shi, L. . A facile one-pot method to prepare peroxidase-like nanogel artificial enzymes for highly efficient and controllable catalysis . Colloid Surf. B-Biointerfaces , 2019 . 174 352 - 359 . DOI: 10.1016/j.colsurfb.2018.11.021 http://doi.org/10.1016/j.colsurfb.2018.11.021 .

Schlüter, A. D.; Rab, J. P . Dendronized polymers: synthesis, characterization, assembly at interfaces, and manipulation . Angew. Chem. Int. Ed. , 2000 . 39 864 - 883 . DOI: 10.1002/(SICI)1521-3773(20000303)39:5<864::AID-ANIE864>3.0.CO;2-E http://doi.org/10.1002/(SICI)1521-3773(20000303)39:5<864::AID-ANIE864>3.0.CO;2-E .

Chen, Y.; Xiong, X. . Tailoring dendronized polymers . Chem. Commun. , 2010 . 46 5049 - 5060 . DOI: 10.1039/b922777f http://doi.org/10.1039/b922777f .

Fuhrmann, G.; Grotzky, A.; Lukic, R.; Matoori, S.; Luciani, P.; Yu, H.; Zhang, B.; Walde, P.; Schluter, A. D.; Gauthier, M. A.; Leroux, J. C. . Sustained gastrointestinal activity of dendronized polymer-enzyme conjugates . Nat. Chem. , 2013 . 5 582 - 589 . DOI: 10.1038/nchem.1675 http://doi.org/10.1038/nchem.1675 .

Zeng, H.; Little, H. C.; Tiambeng, T. N.; Williams, G. A.; Guan, Z. . Multifunctional dendronized peptide polymer platform for safe and effective siRNA delivery . J. Am. Chem. Soc. , 2013 . 135 4962 - 4965 . DOI: 10.1021/ja400986u http://doi.org/10.1021/ja400986u .

Junk, M. J.; Li, W.; Schluter, A. D.; Wegner, G.; Spiess, H. W.; Zhang, A.; Hinderberger, D. . EPR spectroscopic characterization of local nanoscopic heterogeneities during the thermal collapse of thermoresponsive dendronized polymers . Angew. Chem. Int. Ed. , 2010 . 49 5683 - 5687 . DOI: 10.1002/anie.201001469 http://doi.org/10.1002/anie.201001469 .

Liu, L.; Li, W.; Liu, K.; Yan, J.; Hu, G.; Zhang, A. . Comblike thermoresponsive polymers with sharp transitions: synthesis, characterization, and their use as sensitive colorimetric sensors . Macromolecules , 2011 . 44 8614 - 8621 . DOI: 10.1021/ma201874c http://doi.org/10.1021/ma201874c .

Feng, X.; Liu, J.; Xu, G.; Zhang, X.; Su, X.; Li, W.; Zhang, A. . Thermoresponsive double network cryogels from dendronized copolymers showing tunable encapsulation and release of proteins . J. Mater. Chem. B , 2018 . 6 1903 - 1911 . DOI: 10.1039/C7TB03352D http://doi.org/10.1039/C7TB03352D .

Zhou, C.; Abdel-Rahman, M. A.; Li, W.; Liu, K.; Zhang, A. . Thermoresponsive dendronized copolymers for protein recognitions based on biotin-avidin interaction . Chin. Chem. Lett. , 2017 . 28 832 - 838 . DOI: 10.1016/j.cclet.2016.11.016 http://doi.org/10.1016/j.cclet.2016.11.016 .

Yan, J.; Liu, K.; Li, W.; Shi, H.; Zhang, A. . Thermoresponsive dendronized polypeptides showing switchable recognition to catechols . Macromolecules , 2016 . 49 510 - 517 . DOI: 10.1021/acs.macromol.5b02259 http://doi.org/10.1021/acs.macromol.5b02259 .

Stanzl, E. G.; Trantow, B. M.; Vargas, J. R.; Wender, P. A. . Fifteen years of cell-penetrating, guanidinium-rich molecular transporters: basic science, research tools, and clinical applications . Acc. Chem. Res. , 2013 . 46 2944 - 2954 . DOI: 10.1021/ar4000554 http://doi.org/10.1021/ar4000554 .

Li, W.; Zhang, A.; Feldman, K.; Walde, P.; Schluter, A. D. . Thermoresponsive dendronized polymers . Macromolecules , 2010 . 41 3659 - 3667 . .

Funhoff, A. M.; van Nostrum, C. F.; Lok, M. C.; Fretz, M. M.; Crommelin, D. J. A.; Hennink, W. E. . Poly(3-guanidinopropyl methacrylate): a novel cationic polymer for gene delivery . Bioconjugate Chem. , 2004 . 15 1212 - 1220 . DOI: 10.1021/bc049864q http://doi.org/10.1021/bc049864q .

Biltresse, S.; Attolini, M.; Dive, G.; Cordi, A.; Tucker, G. C.; Marchand-Brynaert, J. . Novel RGD-like molecules based on the tyrosine template: design, synthesis, and biological evaluation on isolated integrins α V β 3 / α IIb β 3 and in cellular adhesion tests . Bioorg. Med. Chem. , 2004 . 12 5379 - 5393 . DOI: 10.1016/j.bmc.2004.07.055 http://doi.org/10.1016/j.bmc.2004.07.055 .

Li, W.; Zhang, A.; Schluter, A. D. . Thermoresponsive dendronized polymers with tunable lower critical solution temperatures . Chem. Commun. , 2008 . 5523 - 5525 . .

Ping, Y.; Liu, C.; Zhang, Z.; Liu, K. L.; Chen, J.; Li, J. . Chitosan-graft-(PEI- β -cyclodextrin) copolymers and their supramolecular PEGylation for DNA and siRNA delivery . Biomaterials , 2011 . 32 8328 - 8341 . DOI: 10.1016/j.biomaterials.2011.07.038 http://doi.org/10.1016/j.biomaterials.2011.07.038 .

Liu, L.; Li, W.; Yan, J.; Zhang, A. . Thermoresponsive dendronized polymeric sensors . J. Polym. Sci., Part A: Polym. Chem. , 2014 . 52 1706 - 1713 . DOI: 10.1002/pola.27171 http://doi.org/10.1002/pola.27171 .

Di Virgilio, F. Dr. Jekyll/Mr. . Hyde: the dual role of extracellular ATP . J. Auton. Nerv. Syst. , 2000 . 81 59 - 63 . DOI: 10.1016/S0165-1838(00)00114-4 http://doi.org/10.1016/S0165-1838(00)00114-4 .

Cheng, Q.; Huang, Y.; Zheng, H.; Wei, T.; Zheng, S.; Huo, S.; Wang, X.; Du, Q.; Zhang, X.; Zhang, H. Y.; Liang, X. J.; Wang, C.; Tang, R.; Liang, Z. . The effect of guanidinylation of PEGylated poly(2-aminoethyl methacrylate) on the systemic delivery of siRNA . Biomaterials , 2013 . 34 3120 - 3131 . DOI: 10.1016/j.biomaterials.2013.01.043 http://doi.org/10.1016/j.biomaterials.2013.01.043 .

Okuro, K.; Sasaki, M.; Aida, T. . Boronic acid-appended molecular glues for ATP-responsive activity modulation of enzymes . J. Am. Chem. Soc. , 2016 . 138 5527 - 5530 . DOI: 10.1021/jacs.6b02664 http://doi.org/10.1021/jacs.6b02664 .

浏览量

Downloads

CSCD

文章被引用时，请邮件提醒。

Submit

工具集

关联资源

Synthesis of Helical Poly(phenylacetylene)s Carrying Dendritic Pendants with Varied Branching Densities Through Polymerization in Different Solvents

A ROS-Sensitive Diselenide-Crosslinked Polymeric Nanogel for NIR Controlled Release

Thermoresponsive Fluorescent Semicrystalline Polymers Decorated with Aggregation Induced Emission Luminogens

Sliding Dynamics of Ring Chain on a Rod-Coil Block Copolymer in Rotaxane

2026-9 Contents