Facile Construction of Synergistic <italic style="font-style: italic">β</italic>-Glucosidase and Cellulase Sequential Co-immobilization System for Enhanced Biomass Conversion

Guan Wang; Kai Zhang; Jia-Yi Xin; Chang-Wen Zhao; Yu-Hong Ma; Wan-Tai Yang

doi:10.1007/s10118-020-2437-3

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Facile Construction of Synergistic β-Glucosidase and Cellulase Sequential Co-immobilization System for Enhanced Biomass Conversion

ARTICLE | Updated：2020-06-01

- Facile Construction of Synergistic β-Glucosidase and Cellulase Sequential Co-immobilization System for Enhanced Biomass Conversion
- Facile Construction of Synergistic β-Glucosidase and Cellulase Sequential Co-immobilization System for Enhanced Biomass Conversion
- Chinese Journal of Polymer Science 2020年38卷第12期页码：1277-1285
- Affiliations：
  
  a.State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, Beijing 100029, China
  b.Beijing Laboratory of Biomedical Materials, Beijing University of Chemical Technology, Beijing 100029, China
  c.Beijing Advanced Innovation Centre for Soft Matter Science and Engineering, Beijing University of Chemical Technology, Beijing 100029, China
  d.Key Laboratory of Carbon Fiber and Functional Polymers, Ministry of Education, Beijing University of Chemical Technology, Beijing 100029, China
- Author bio：
  
  zhaocw@mail.buct.edu.cn (C.W.Z.)
  yangwt@mail.buct.edu.cn (W.T.Y.)
- Funds：
  
  This work was financially supported by the National Natural Science Foundation of China (Nos. 51873014, 51521062, and 51473015).
- DOI：10.1007/s10118-020-2437-3
  中图分类号：
- 收稿：2020-03-08，
  
  修回：2020-3-29，
  
  录用：2020-3-30，
  
  网络首发：2020-06-01，
  
  纸质出版：2020-09-10
- Accepted：
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Wang, G.; Zhang, K.; Xin, J. Y.; Zhao, C. W.; Ma, Y. H.; Yang, W. T. Facile construction of synergistic , β,-glucosidase and cellulase sequential co-immobilization system for enhanced biomass conversion. Chinese J. Polym. Sci. 2020, 38, 1277–1285

Guan Wang, Kai Zhang, Jia-Yi Xin, et al. Facile Construction of Synergistic β-Glucosidase and Cellulase Sequential Co-immobilization System for Enhanced Biomass Conversion[J]. Chinese Journal of Polymer Science, 2020, 38(12): 1277-1285. DOI: 10.1007/s10118-020-2437-3.
Wang, G.; Zhang, K.; Xin, J. Y.; Zhao, C. W.; Ma, Y. H.; Yang, W. T. Facile construction of synergistic , β,-glucosidase and cellulase sequential co-immobilization system for enhanced biomass conversion. Chinese J. Polym. Sci. 2020, 38, 1277–1285 DOI： 10.1007/s10118-020-2437-3.

Guan Wang, Kai Zhang, Jia-Yi Xin, et al. Facile Construction of Synergistic β-Glucosidase and Cellulase Sequential Co-immobilization System for Enhanced Biomass Conversion[J]. Chinese Journal of Polymer Science, 2020, 38(12): 1277-1285. DOI: 10.1007/s10118-020-2437-3. DOI：

摘要

Abstract

Converting renewable cellulose into glucose

via

cellulase catalysis for further production of biofuel has been recognized as one of the most promising ways for solving energy crisis. However

the hydrolysis performance of immobilized cellulase was not satisfactory for practical application due to the reduced catalytic efficiency and lack of

-glucosidase (BG) component in cellulase. Here

a facile method was developed to sequentially co-immobilize BG and cellulase by polymeric microparticles with hierarchical structure. In this strategy

BG was firstly entrapped into the cross-linked poly(ethylene glycol) (PEG) microparticles

via

inverse emulsion polymerization initiated by isopropyl thioxanthone (ITX) under the irradiation of visible light

leaving the formed ITX semi-pinacol (ITXSP) dormant groups on surface of BG-loaded microparticles

which could be further activated by visible light irradiation and initiated a graft polymerization to introduce poly(acrylic acid) (PAA) brush on the PEG core. After that

cellulase was covalently bonded on the PAA chains

via

carbodiimide reaction. The synergic effect of BG and cellulase was verified in the dual enzyme immobilization system

which led to a better stability at a wide range of temperature and pH than free enzymes. The dual enzymes system exhibited excellent reusability

which could retain 75% and 57% of the initial activity after 10 cycles of hydrolysis of carboxyl methyl cellulose and 5 cycles of hydrolysis of filter paper

respectively

indicative of the potential in biofuel areas in a cost-effective manner.

关键词

Keywords

references

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DOI：10.1007/s10118-020-2437-3

摘要

Abstract

关键词

Keywords

references