Effects of Branching Strategy on the Gene Transfection of Highly Branched Poly(<italic style="font-style: italic">β</italic>-amino ester)s

Yao Wang; Chen-Fei Wang; Ming Li; De-Zhong Zhou; Wei Huang; Wen-Xin Wang

doi:10.1007/s10118-020-2393-y

Your Location：

Home >

Browse articles >

Effects of Branching Strategy on the Gene Transfection of Highly Branched Poly(β-amino ester)s

ARTICLE | Updated：2020-04-17

- Effects of Branching Strategy on the Gene Transfection of Highly Branched Poly(β-amino ester)s
- Effects of Branching Strategy on the Gene Transfection of Highly Branched Poly(β-amino ester)s
- Chinese Journal of Polymer Science 2020年38卷第8期页码：830-839
- Affiliations：
  
  a.School of Chemistry and Chemical Engineering, State Key Laboratory of Metal Matrix Composites, Shanghai Jiao Tong University, Shanghai 200240, China
  b.School of Chemical Engineering and Technology, Xi'an Jiaotong University, Xi'an 710049, China
  c.Charles Institute of Dermatology, School of Medicine, University College Dublin, Dublin 4, Ireland
  d.School of Mechanical and Materials Engineering, University College Dublin, Dublin 4, Ireland
  e.Department of Dermatology, Xinhua Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China
- Author bio：
  
  dezhong.zhou@xjtu.edu.cn (D.Z.Z.)
  wenxin.wang@ucd.ie (W.X.W.)
- Funds：
  
  This work was financially supported by Science Foundation Ireland (SFI) Principal Investigator Program (No. 13/IA/1962), the National Natural Science Foundation of China (Nos. 51873179 and 51903202), and University College Dublin.
- DOI：10.1007/s10118-020-2393-y
  中图分类号：
- 收稿：2019-12-21，
  
  修回：2020-1-12，
  
  录用：2020-1-17，
  
  网络首发：2020-04-17，
  
  纸质出版：2020-08
- Accepted：
Scan QR Code
Wang, Y.; Wang, C. F.; Li, M.; Zhou, D. Z.; Huang W.; Wang, W. X. Effects of branching strategy on the gene transfection of highly branched poly(, β,-amino ester)s. Chinese J. Polym. Sci. 2020, 38, 830–839

Yao Wang, Chen-Fei Wang, Ming Li, et al. Effects of Branching Strategy on the Gene Transfection of Highly Branched Poly(β-amino ester)s[J]. Chinese Journal of Polymer Science, 2020, 38(8): 830-839. DOI: 10.1007/s10118-020-2393-y.
Wang, Y.; Wang, C. F.; Li, M.; Zhou, D. Z.; Huang W.; Wang, W. X. Effects of branching strategy on the gene transfection of highly branched poly(, β,-amino ester)s. Chinese J. Polym. Sci. 2020, 38, 830–839 DOI： 10.1007/s10118-020-2393-y.

Yao Wang, Chen-Fei Wang, Ming Li, et al. Effects of Branching Strategy on the Gene Transfection of Highly Branched Poly(β-amino ester)s[J]. Chinese Journal of Polymer Science, 2020, 38(8): 830-839. DOI: 10.1007/s10118-020-2393-y. DOI：

摘要

Abstract

Highly branched poly(

-amino ester)s (HPAEs) have emerged as one type of the most viable non-viral gene delivery vectors

both

in vitro

and

in vivo

. However

the effects of different branching strategies on the gene transfection performance have not yet been explored. Here

using triacrylate (B3) and diamine (B4) as the branching monomers

a series of HPAEs were synthesized

via

the “A2 + B3 + C2” and “A2 + B4 + C2” strategies

respectively. Results show that the branching strategy plays a pivotal role in dictating the physiological properties of the HPAE/DNA polyplexes and thus leads to obviously different cell viability and transfection efficiency. Comparatively

HPAEs synthesized

via

the “A2 + B3 + C2” branching strategy are more favorable for DNA transfection than that synthesized

via

the “A2 + B4 + C2” strategy. This study may provide new insights into the development of HPAEs based non-viral DNA delivery system.

关键词

Keywords

references

Pack, D. W.; Hoffman, A. S.; Pun, S.; Stayton, P. S. . Design and development of polymers for gene delivery . Nat. Rev. Drug Discov. , 2005 . 4 581 - 593 . DOI: 10.1038/nrd1775 http://doi.org/10.1038/nrd1775 .

Nguyen, J.; Szoka, F. C. . Nucleic acid delivery: the missing pieces of the puzzle? . Acc. Chem. Res. , 2012 . 45 1153 - 1162 . DOI: 10.1021/ar3000162 http://doi.org/10.1021/ar3000162 .

Behr, J. P. . Synthetic gene-transfer vectors . Acc. Chem. Res. , 1993 . 26 274 - 278 . DOI: 10.1021/ar00029a008 http://doi.org/10.1021/ar00029a008 .

Srinivas, R.; Samanta, S.; Chaudhuri, A. . Cationic amphiphiles: promising carriers of genetic materials in gene therapy . Chem. Soc. Rev. , 2009 . 38 3326 - 3338 . DOI: 10.1039/b813869a http://doi.org/10.1039/b813869a .

Mintzer, M. A.; Simanek, E. E. . Nonviral vectors for gene delivery . Chem. Rev. , 2009 . 109 259 - 302 . DOI: 10.1021/cr800409e http://doi.org/10.1021/cr800409e .

Guo, X.; Huang, L. . Recent advances in non-viral vectors for gene delivery . Acc. Chem. Res. , 2013 . 45 971 - 979 . .

Boussif, O.; Lezoualc'h, F; Zanta, M. A.; Mergny, M. D.; Scherman, D.; Demeneix, B.; Behr, J. P. . A versatile vector for gene and oligonucleotide transfer into cells in culture and in vivo : polyethylenimine . Proc. Natl. Acad. Sci. , 1995 . 92 7297 - 7301 . DOI: 10.1073/pnas.92.16.7297 http://doi.org/10.1073/pnas.92.16.7297 .

Liu, H.; Wang, H.; Yang, W.; Cheng, Y. . Disulfide cross-linked low generation dendrimers with high gene transfection efficacy, low cytotoxicity, and low cost . J. Am. Chem. Soc. , 2012 . 134 17680 - 17687 . DOI: 10.1021/ja307290j http://doi.org/10.1021/ja307290j .

Zhao, T.; Zhang, H.; Newland, B.; Aied, A.; Zhou, D.; Wang, W. . Significance of branching for transfection: synthesis of highly branched degradable functional poly(dimethylaminoethyl methacrylate) by vinyl oligomer combination . Angew. Chem. Int. Ed. , 2014 . 53 6095 - 6100 . DOI: 10.1002/anie.201402341 http://doi.org/10.1002/anie.201402341 .

Zhou, D.; Li, C.; Hu, Y.; Zhou, H.; Chen, J.; Zhang, Z.; Guo, T. . The effects of a multifunctional oligomer and its incorporation strategies on the gene delivery efficiency of poly(L-lysine) . Chem. Commun. , 2012 . 48 4594 DOI: 10.1039/c2cc31359f http://doi.org/10.1039/c2cc31359f .

Li, C.; Guo, T.; Zhou, D.; Hu, Y.; Zhou, H.; Wang, S.; Chen, J.; Zhang, Z. . A novel glutathione modified chitosan conjugate for efficient gene delivery . J. Control. Release , 2011 . 154 177 - 188 . DOI: 10.1016/j.jconrel.2011.06.007 http://doi.org/10.1016/j.jconrel.2011.06.007 .

Liu, S.; Zhou, D.; Yang, J.; Zhou, H.; Chen, J.; Guo, T. . Bioreducible zinc(II)-coordinative polyethylenimine with low molecular weight for robust gene delivery of primary and stem cells . J. Am. Chem. Soc. , 2017 . 139 5102 - 5109 . DOI: 10.1021/jacs.6b13337 http://doi.org/10.1021/jacs.6b13337 .

Wang, M.; Liu, H.; Li, L.; Cheng, Y. . A fluorinated dendrimer achieves excellent gene transfection efficacy at extremely low nitrogen to phosphorus ratios . Nat. Commun. , 2014 . 5 3053 DOI: 10.1038/ncomms4053 http://doi.org/10.1038/ncomms4053 .

Lynn, D. M.; Langer, R. . Degradable poly( β -amino esters): synthesis, characterization, and self-assembly with plasmid DNA . J. Am. Chem. Soc. , 2000 . 122 10761 - 10768 . DOI: 10.1021/ja0015388 http://doi.org/10.1021/ja0015388 .

Green, J. J.; Langer, R.; Anderson, D. G. . Yields Insight into nonviral gene delivery . Acc. Chem. Res. , 2008 . 41 749 - 759 . DOI: 10.1021/ar7002336 http://doi.org/10.1021/ar7002336 .

Akinc, A.; Lynn, D. M.; Anderson, D. G.; Langer, R. . Parallel synthesis and biophysical characterization of a degradable polymer library for gene delivery . J. Am. Chem. Soc. , 2003 . 125 5316 - 5323 . DOI: 10.1021/ja034429c http://doi.org/10.1021/ja034429c .

Eltoukhy, A. A.; Siegwart, D. J.; Alabi, C. A.; Rajan, J. S.; Langer, R.; Anderson, D. G. . Effect of molecular weight of amine end-modified poly( β -amino ester)s on gene delivery efficiency and toxicity . Biomaterials , 2012 . 33 3594 - 3603 . DOI: 10.1016/j.biomaterials.2012.01.046 http://doi.org/10.1016/j.biomaterials.2012.01.046 .

Anderson, D. G.; Lynn, D. M.; Langer, R. . Semi-automated synthesis and screening of a large library of degradable cationic polymers for gene delivery . Angew. Chem. Int. Ed. , 2003 . 42 3153 - 3158 . DOI: 10.1002/anie.200351244 http://doi.org/10.1002/anie.200351244 .

Green, J. J.; Zugates, G. T.; Tedford, N. C.; Huang, Y. H.; Griffith, L. G.; Lauffenburger, D. A.; Sawicki, J. A.; Langer, R.; Anderson, D. G. . Combinatorial modification of degradable polymers enables transfection of human cells comparable to adenovirus . Adv. Mater. , 2007 . 19 2836 - 2842 . DOI: 10.1002/adma.200700371 http://doi.org/10.1002/adma.200700371 .

Lee, C. C.; MacKay, J. A.; Fréchet, J. M. J.; Szoka, F. C. . Designing dendrimers for biological applications . Nat. Biotechnol. , 2005 . 23 1517 - 1526 . DOI: 10.1038/nbt1171 http://doi.org/10.1038/nbt1171 .

Voit, B. I.; Lederer, A. . Hyperbranched and highly branched polymer architectures-synthetic strategies and major characterization aspects . Chem. Rev. , 2009 . 109 5924 - 5973 . DOI: 10.1021/cr900068q http://doi.org/10.1021/cr900068q .

Lim, Y. B.; Kim, S. M.; Lee, Y.; Lee, W. K.; Yang, T. G.; Lee, M. J.; Suh, H.; Park, J. S. . Cationic hyperbranched poly(amino ester): a novel class of DNA condensing molecule with cationic surface, biodegradable three-dimensional structure, and tertiary amine groups in the interior . J. Am. Chem. Soc. , 2001 . 123 2460 - 2461 . DOI: 10.1021/ja005715g http://doi.org/10.1021/ja005715g .

Wu, D.; Liu, Y.; Chen, L.; He, C.; Chung, T. S.; Goh, S. H. . 2A 2 + BB′B′′ approach to hyperbranched poly(amino ester)s . Macromolecules , 2005 . 38 5519 - 5525 . DOI: 10.1021/ma047580v http://doi.org/10.1021/ma047580v .

Liu, Y.; Wu, D.; Ma, Y.; Tang, G.; Wang, S.; He, C.; Chung, T.; Goh, S . Novel poly(amino ester)s obtained from Michael addition polymerizations of trifunctional amine monomers with diacrylates: safe and efficient DNA carriers . Chem. Commun. , 2003 . 20 2630 - 2631 . DOI: 10.1039/B309487A http://doi.org/10.1039/B309487A .

Wu, D.; Liu, Y.; Jiang, X.; He, C.; Goh, S. H.; Leong, K. W. . Hyperbranched poly(amino ester)s with different terminal amine groups for DNA delivery . Biomacromolecules , 2006 . 7 1879 - 1883 . DOI: 10.1021/bm0601878 http://doi.org/10.1021/bm0601878 .

Huang, J. Y.; Gao, Y.; Cutlar, L.; O'Keeffe-Ahern, J.; Zhao, T.; Lin, F. H.; Zhou, D.; McMahon, S.; Greiser, U.; Wang, W.; Wang W. . Tailoring highly branched poly( β -amino ester)s: a synthetic platform for epidermal gene therapy . Chem. Commun. , 2015 . 51 8473 - 8476 . DOI: 10.1039/C5CC02193F http://doi.org/10.1039/C5CC02193F .

Zhou, D.; Cutlar, L.; Gao, Y.; Wang, W.; O’Keeffe-Ahern, J.; McMahon, S.; Duarte, B.; Larcher, F.; Rodriguez, J. B.; Greiser U.; Wang, W. . Highly branched poly( β -amino esters): synthesis and application in gene delivery . Biomacromolecules , 2015 . 16 2609 - 2617 . DOI: 10.1021/acs.biomac.5b00966 http://doi.org/10.1021/acs.biomac.5b00966 .

Zhou, D.; Cutlar, L.; Gao, Y.; Wang, W.; O’Keeffe-Ahern, J.; McMahon, S.; Duarte, B.; Larcher, F.; Rodriguez, J. B.; Greiser U.; Wang, W. . The transition from linear to highly branched poly( β -amino ester)s: branching matters for gene delivery . Sci. Adv. , 2016 . 2 e1600102 DOI: 10.1126/sciadv.1600102 http://doi.org/10.1126/sciadv.1600102 .

Zhou, D.; Gao, Y.; Aied, A.; Cutlar, L.; Igoucheva, O.; Newland, B.; Alexeeve, V.; Greiser, U.; Uitto, J.; Wang, W. . Highly branched poly( β -amino ester)s for skin gene therapy . J. Control. Release , 2016 . 244 336 - 346 . DOI: 10.1016/j.jconrel.2016.06.014 http://doi.org/10.1016/j.jconrel.2016.06.014 .

Cutlar, L.; Zhou, D.; Hu, X.; Duarte, B.; Greiser, U.; Larcher, F.; Wang, W. . A non-viral gene therapy for treatment of recessive dystrophic epidermolysis bullosa . Exp. Dermatol. , 2016 . 25 818 - 820 . DOI: 10.1111/exd.13054 http://doi.org/10.1111/exd.13054 .

Zeng, M.; Zhou, D.; Alshehri, F.; Lara-Sáez, I.; Lyu, Y.; Creagh-Flynn, J.; A,; S.;, Xu Q.; Zhang, J.; Wang W. . Manipulation of transgene expression in fibroblast cells by a multifunctional linear-branched hybrid poly( β -amino ester) synthesized through an oligomer combination approach . Nano Lett. , 2019 . 19 381 - 391 . DOI: 10.1021/acs.nanolett.8b04098 http://doi.org/10.1021/acs.nanolett.8b04098 .

Zeng, M.; Alshehri, F.; Zhou, D.; Lara-Sáez, I.; Wang, X.; Li, X. A.; Xu, Q.; Zhang, J.; Wang W. . Efficient and robust highly branched poly( β -amino ester)/minicircle COL7A1 polymeric nanoparticles for gene delivery to recessive dystrophic epidermolysis bullosa keratinocytes . ACS Appl. Mater. Interfaces , 2019 . 11 30661 - 30672 . DOI: 10.1021/acsami.9b13135 http://doi.org/10.1021/acsami.9b13135 .

Zhou, D.; Gao, Y.; Ahern, J. O.; Xu, Q.; Huang, X.; Greiser, U.; Wang, W. . Development of branched poly(5-amino-1-pentanol- co -1,4-butanediol diacrylate) with high gene transfection potency across diverse cell types . ACS Appl. Mater. Interfaces , 2016 . 8 34218 - 34226 . DOI: 10.1021/acsami.6b12078 http://doi.org/10.1021/acsami.6b12078 .

Liu, S.; Gao, Y.; Zhou, D.; Zeng, M.; Alshehri, F.; Newland, B.; Lyu, J.; O’Keeffe-Ahern, J.; Greiser, U.; Guo, T.; Zhang, F.; Wang, W. . Highly branched poly( β -amino ester) delivery of minicircle DNA for transfection of neurodegenerative disease related cells . Nat. Commun. , 2019 . 10 1 - 14 . DOI: 10.1038/s41467-018-07882-8 http://doi.org/10.1038/s41467-018-07882-8 .

Zeng, M.; Zhou, D.; Ng, S.; Ahern, J. O.; Alshehri, F.; Gao, Y.; Pierucci, L.; Greiser U.; Wang, W. . Highly branched poly(5-amino-1-pentanol- co -1,4-butanediol diacrylate) for high performance gene transfection . Polymers , 2017 . 9 161 DOI: 10.3390/polym9050161 http://doi.org/10.3390/polym9050161 .

Liu, S.; Sun, Z.; Zhou, D.; Guo, T. . Alkylated branched poly( β -amino esters) demonstrate strong DNA encapsulation, high nanoparticle stability and robust gene transfection efficacy . J. Mater. Chem. B , 2017 . 5 5307 - 5310 . DOI: 10.1039/C7TB00996H http://doi.org/10.1039/C7TB00996H .

Gao, Y.; Huang, J. Y.; Ahern, J. O.; Cutlar, L.; Zhou, D.; Lin, F. H.; Wang, W. . Highly branched poly( β -amino esters) for non-viral gene delivery: high transfection efficiency and low toxicity achieved by increasing molecular weight . Biomacromolecules , 2016 . 17 3640 - 3647 . DOI: 10.1021/acs.biomac.6b01120 http://doi.org/10.1021/acs.biomac.6b01120 .

Liu, S.; Gao, Y.; Zhou, D.; Greiser, U.; Guo, T.; Guo, R.; Wang, W. . Biodegradable highly branched poly( β -amino ester)s for targeted cancer cell gene transfection . ACS Biomater. Sci. Eng. , 2017 . 3 1283 - 1286 . DOI: 10.1021/acsbiomaterials.6b00503 http://doi.org/10.1021/acsbiomaterials.6b00503 .

Sperling, L. H. Introduction to physical polymer science , 4 th ed. John Wiley & amp; Sons, 2005 .

Anderson, D. G.; Peng, W.; Akinc, A.; Hossain, N.; Kohn, A.; Padera, R.; Langer, R.; Sawicki, J. A. . A polymer library approach to suicide gene therapy for cancer . Proc. Natl. Acad. Sci. U. S. A. , 2004 . 101 16028 - 16033 . DOI: 10.1073/pnas.0407218101 http://doi.org/10.1073/pnas.0407218101 .

Newland, B.; Zheng, Y.; Jin, Y.; Abu-Rub, M.; Cao, H.; Wang, W.; Pandit, A. . Single cyclized molecule versus single branched molecule: a simple and efficient 3D ‘knot’ polymer structure for nonviral gene delivery . J. Am. Chem. Soc. , 2012 . 134 4782 - 4789 . DOI: 10.1021/ja2105575 http://doi.org/10.1021/ja2105575 .

Xiang, S.; Tong, H.; Shi, Q.; Fernandes, J. C.; Jin, T.; Dai, K.; Zhang, X. . Uptake mechanisms of non-viral gene delivery . J. Control. Release , 2012 . 158 371 - 378 . DOI: 10.1016/j.jconrel.2011.09.093 http://doi.org/10.1016/j.jconrel.2011.09.093 .

Liu, Z.; Zhang, Z.; Zhou, C.; Jiao, Y. . Hydrophobic modifications of cationic polymers for gene delivery . Prog. Polym. Sci. , 2010 . 35 1144 - 1162 . DOI: 10.1016/j.progpolymsci.2010.04.007 http://doi.org/10.1016/j.progpolymsci.2010.04.007 .

浏览量

Downloads

CSCD

文章被引用时，请邮件提醒。

Submit

工具集

关联资源

WHAT CAN WE LEARN FROM VIRUS IN DESIGNING NONVIRAL GENE VECTORS

Tunable Circularly Polarized Luminescence of Helical Poly(phenylacetylene)s via Side-chain-to-Backbone Förster Resonance Energy Transfer

Epoxy-functionalized Cyclohexane-based Ricinoleate Plasticizer: Synthesis, Performance Evaluation, and Plasticizing Mechanism

Enhancing Processability of Phenylethynyl-terminated Imide Oligomers and Properties of Cured Polyimides via ―CF3 Incorporation

Preparation and Chiral Recognition Ability of Crystalline Cellulose Phenylcarbamate

Effects of Branching Strategy on the Gene Transfection of Highly Branched Poly(β-amino ester)s

Effects of Branching Strategy on the Gene Transfection of Highly Branched Poly(β-amino ester)s

DOI：10.1007/s10118-020-2393-y

摘要

Abstract

关键词

Keywords

references