Single-wavelength Excited Ratiometric Fluorescence pH Probe to Image Intracellular Trafficking of Tobacco Mosaic Virus

Si-Jia Gao; Zhuang Li; Zhi-Cheng Sun; Jin-Yue Wen; Fu-Rong Li; Xiao-Yang Du; Yan Liu; Ye Tian; Zhong-Wei Niu

doi:10.1007/s10118-020-2365-2

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Single-wavelength Excited Ratiometric Fluorescence pH Probe to Image Intracellular Trafficking of Tobacco Mosaic Virus

ARTICLE | Updated：2019-12-05

- Single-wavelength Excited Ratiometric Fluorescence pH Probe to Image Intracellular Trafficking of Tobacco Mosaic Virus
- Single-wavelength Excited Ratiometric Fluorescence pH Probe to Image Intracellular Trafficking of Tobacco Mosaic Virus
- Chinese Journal of Polymer Science 2020年38卷第6期页码：587-592
- Affiliations：
  
  a.Key Laboratory of Photochemical Conversion and Optoelectronic Materials, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing 100190, China
  b.University of Chinese Academy of Sciences, Beijing 100049, China
  c.Beijing Engineering Research Center of Printed Electronics, Beijing Institute of Graphic Communication, Beijing 102600, China
  d.School of Future Technology, University of Chinese Academy of Sciences, Beijing 100049, China
- Author bio：
  
  tiany@mail.ipc.ac.cn (Y.T.)
  niu@mail.ipc.ac.cn (Z.W.N.)
- Funds：
  
  This work was financially supported by the National Key R&D Program of China (No. 2018YFC1105300), the National Natural Science Foundation of China (Nos. 51703230 and 21776021), the Beijing Natural Science Foundation (No. 7182110), the Cross Training Plan for High Level Talents in Beijing, the Youth Innovation Promotion Association of the Chinese Academy of Sciences (No. 2017039), and the Presidential Foundation of Technical Institute of Physics and Chemistry.
- DOI：10.1007/s10118-020-2365-2
  中图分类号：
- 收稿：2019-09-11，
  
  修回：2019-10-14，
  
  录用：2019-10-21，
  
  网络首发：2019-12-05，
  
  纸质出版：2020-06
- Accepted：
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Gao, S. J.; Li, Z.; Sun, Z. C.; Wen, J. Y.; Li, F. R.; Du, X. Y.; Liu, Y.; Tian, Y.; Niu, Z. W. Single-wavelength excited ratiometric fluorescence pH probe to image intracellular trafficking of tobacco mosaic virus. Chinese J. Polym. Sci. 2020, 38, 587–592

Si-Jia Gao, Zhuang Li, Zhi-Cheng Sun, et al. Single-wavelength Excited Ratiometric Fluorescence pH Probe to Image Intracellular Trafficking of Tobacco Mosaic Virus[J]. Chinese Journal of Polymer Science, 2020, 38(6): 587-592.
Gao, S. J.; Li, Z.; Sun, Z. C.; Wen, J. Y.; Li, F. R.; Du, X. Y.; Liu, Y.; Tian, Y.; Niu, Z. W. Single-wavelength excited ratiometric fluorescence pH probe to image intracellular trafficking of tobacco mosaic virus. Chinese J. Polym. Sci. 2020, 38, 587–592 DOI： 10.1007/s10118-020-2365-2.

Si-Jia Gao, Zhuang Li, Zhi-Cheng Sun, et al. Single-wavelength Excited Ratiometric Fluorescence pH Probe to Image Intracellular Trafficking of Tobacco Mosaic Virus[J]. Chinese Journal of Polymer Science, 2020, 38(6): 587-592. DOI： 10.1007/s10118-020-2365-2.

摘要

Abstract

As a typical plant virus which has biocompatibility and high transfection efficiency

tobacco mosaic virus (TMV) has shown broad application potential in drug or gene delivery field. Elucidating its intracellular trafficking is of great importance in investigation of its cytotoxicity

targeting site

and delivery efficiency

and is advantageous to designing new TMV-based drug delivery systems with different targets. By taking advantage of the regulated pH value of different organelles in a mammalian cell

we exploit a pH detection strategy to investigate the intracellular trafficking pathway of TMV. Here

we report a single-wavelength excited ratiometric fluorescent pH probe. This probe is constructed by simultaneously coupling pH-sensitive fluorescein isothiocyanate (FITC) and pH-insensitive rhodamine B isothiocyanate (RBIRC) onto the inner surface of TMV. The fluorescence intensity ratio of FITC to RBITC excited at 488 nm responds specifically towards pH value over other interferential agents. By taking use of this single-wavelength excited ratiometric pH probe and confocal laser scanning microscopy

it is shown that the endocytosed TMV is located in a pH decreasing microenvironment and eventually enters lysosomes. This work may provide important guidance on construction of TMV-based nano carriers.

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Keywords

references

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