Mediating the Migration of Mesenchymal Stem Cells by Dynamically Changing the Density of Cell-selective Peptides Immobilized on <italic style="font-style: italic">β</italic>-Cyclodextrin-modified Cell-resisting Polymer Brushes

Wang Du; De-Teng Zhang; Xue-Mei Wang; Tan-Chen Ren; Chang-You Gao

doi:10.1007/s10118-019-2324-y

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Mediating the Migration of Mesenchymal Stem Cells by Dynamically Changing the Density of Cell-selective Peptides Immobilized on β-Cyclodextrin-modified Cell-resisting Polymer Brushes

ARTICLE | Updated：2019-09-27

- Mediating the Migration of Mesenchymal Stem Cells by Dynamically Changing the Density of Cell-selective Peptides Immobilized on β-Cyclodextrin-modified Cell-resisting Polymer Brushes
- Mediating the Migration of Mesenchymal Stem Cells by Dynamically Changing the Density of Cell-selective Peptides Immobilized on β-Cyclodextrin-modified Cell-resisting Polymer Brushes
- Chinese Journal of Polymer Science 2020年38卷第2期页码：126-136
- Affiliations：
  
  Ministry of Education Key Laboratory of Macromolecular Synthesis and Functionalization, Department of Polymer Science and Engineering, Zhejiang University, Hangzhou 310027, China
- Author bio：
  
  cygao@zju.edu.cn
- Funds：
  
  This work was financially supported by the National Key Research and Development Program of China (No. 2016YFC1100403) and the National Natural Science Foundation of China (Nos. 21434006 and 51873188).
- DOI：10.1007/s10118-019-2324-y
  中图分类号：
- 收稿：2019-05-26，
  
  修回：2019-6-15，
  
  录用：2019-6-26，
  
  网络首发：2019-09-27，
  
  纸质出版：2020-02
- Accepted：
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Du, W.; Zhang, D. T.; Wang, X. M.; Ren, T. C.; Gao, C. Y. Mediating the migration of mesenchymal stem cells by dynamically changing the density of cell-selective peptides immobilized on , β,-cyclodextrin-modified cell-resisting polymer brushes. Chinese J. Polym. Sci. 2020, 38, 126–136.

Wang Du, De-Teng Zhang, Xue-Mei Wang, et al. Mediating the Migration of Mesenchymal Stem Cells by Dynamically Changing the Density of Cell-selective Peptides Immobilized on β-Cyclodextrin-modified Cell-resisting Polymer Brushes[J]. Chinese Journal of Polymer Science, 2020, 38(2): 126-136. DOI: 10.1007/s10118-019-2324-y.
Du, W.; Zhang, D. T.; Wang, X. M.; Ren, T. C.; Gao, C. Y. Mediating the migration of mesenchymal stem cells by dynamically changing the density of cell-selective peptides immobilized on , β,-cyclodextrin-modified cell-resisting polymer brushes. Chinese J. Polym. Sci. 2020, 38, 126–136. DOI： 10.1007/s10118-019-2324-y.

Wang Du, De-Teng Zhang, Xue-Mei Wang, et al. Mediating the Migration of Mesenchymal Stem Cells by Dynamically Changing the Density of Cell-selective Peptides Immobilized on β-Cyclodextrin-modified Cell-resisting Polymer Brushes[J]. Chinese Journal of Polymer Science, 2020, 38(2): 126-136. DOI: 10.1007/s10118-019-2324-y. DOI：

摘要

Abstract

Dynamic control of mesenchymal stem cell (MSC) behaviors on biomaterial surface is critically involved in regulating the cell fate and tissue regeneration. Herein

a stimuli-responsive surface based on host-guest interaction with cell selectivity was developed to regulate migration of MSCs

in situ

by dynamic display of cell-specific peptides. Azobenzene-grafted MSC-affinitive peptides (EPLQLKM

Azo-E7) were grafted to

-cyclodextran (

-CD)-modified poly(2-hydroxyethyl methacrylate)-

-poly(2-hydroxyethyl methacrylate-

-glycidyl methacrylate) (PHG) brushes

which were prepared by using surface-initiated atom transfer radical polymerization (SI-ATRP). X-ray photoelectron spectroscopy (XPS)

quartz crystal microbalance (QCM)

and water contact angle were used to characterize their structure and property. Cell adhesion assay showed that the combination effect of resisting property of PHG and MSC-affinity of E7 could promote the selective adhesion of MSCs over other types of cells such as RAW264.7 macrophages and NIH3T3 fibroblasts to some extent. UV-Vis spectroscopy proved that the competing guest molecules

amantadine hydrochloride (Ama)

could release Azo-E7 peptides from the CD surface to different extents

and the effect was enhanced when UV irradiation was employed simultaneously. As a result

the decrease of cell adhesion density and migration rate could be achieved

in situ

. The cell density and migration rate could be reduced by over 40% by adding 20 μmol/L Ama

suggesting that this type of surface is a new platform for dynamic regulation of stem cell behaviors

in situ

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references

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Mediating the Migration of Mesenchymal Stem Cells by Dynamically Changing the Density of Cell-selective Peptides Immobilized on β-Cyclodextrin-modified Cell-resisting Polymer Brushes

DOI：10.1007/s10118-019-2324-y

摘要

Abstract

关键词

Keywords

references