Biofilm Disruption Utilizing <italic style="font-style: italic">α</italic>/<italic style="font-style: italic">β</italic> Chimeric Polypeptide Molecular Brushes

Si Zhang; Xi-Mian Xiao; Fan Qi; Peng-Cheng Ma; Wei-Wei Zhang; Cheng-Zhi Dai; Dan-Feng Zhang; Run-Hui Liu

doi:10.1007/s10118-019-2278-0

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Biofilm Disruption Utilizing α/β Chimeric Polypeptide Molecular Brushes

ARTICLE | Updated：2021-02-18

- Biofilm Disruption Utilizing α/β Chimeric Polypeptide Molecular Brushes
- Biofilm Disruption Utilizing α/β Chimeric Polypeptide Molecular Brushes
- Chinese Journal of Polymer Science 2019年37卷第11期页码：1105-1112
- Affiliations：
  
  State Key Laboratory of Bioreactor Engineering, Key Laboratory for Ultrafine Materials of Ministry of Education, Research Center for Biomedical Materials of Ministry of Education, School of Materials Science and Engineering, East China University of Science and Technology, Shanghai 200237, China
- Author bio：
  
  rliu@ecust.edu.cn
- Funds：
  
  This work was financially supported by the National Natural Science Foundation of China (Nos. 21574038, 21774031, and 21861162010), the Natural Science Foundation of Shanghai (No. 18ZR1410300), the “Eastern Scholar Professorship” from Shanghai local government (No. TP2014034), the national special fund for State Key Laboratory of Bioreactor Engineering (No. 2060204), and the Fundamental Research Funds for the Central Universities (No. 22221818014).
- DOI：10.1007/s10118-019-2278-0
  中图分类号： M
- 收稿：2019-03-13，
  
  修回：2019-4-13，
  
  录用：2019-4-18，
  
  网络首发：2019-06-17，
  
  纸质出版：2019-11
- Accepted：
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Zhang, S.; Xiao, X. M.; Qi, F.; Ma, P. C.; Zhang, W. W.; Dai, C. Z.; Zhang, D. F.; Liu, R. H. Biofilm disruption utilizing α/β chimeric polypeptide molecular brushes. Chinese J. Polym. Sci. 2019, 37, 1105–1112.

Si Zhang, Xi-Mian Xiao, Fan Qi, et al. Biofilm Disruption Utilizing α/β Chimeric Polypeptide Molecular Brushes[J]. Chinese Journal of Polymer Science, 2019, 37(11): 1105-1112. DOI: 10.1007/s10118-019-2278-0.
Zhang, S.; Xiao, X. M.; Qi, F.; Ma, P. C.; Zhang, W. W.; Dai, C. Z.; Zhang, D. F.; Liu, R. H. Biofilm disruption utilizing α/β chimeric polypeptide molecular brushes. Chinese J. Polym. Sci. 2019, 37, 1105–1112. DOI： 10.1007/s10118-019-2278-0.

Si Zhang, Xi-Mian Xiao, Fan Qi, et al. Biofilm Disruption Utilizing α/β Chimeric Polypeptide Molecular Brushes[J]. Chinese Journal of Polymer Science, 2019, 37(11): 1105-1112. DOI: 10.1007/s10118-019-2278-0. DOI：

摘要

Abstract

Gram-negative bacteria can cause serious infections and are well known problems in biomedical practices. Biofilms of gram-negative bacteria are notorious for their frequently encountered resistance toward antibiotics. We demonstrate that

chimeric polypeptide molecular brush (

CPMB) exerts potent activities against antibiotic-resistant gram-negative bacteria. MTT viability assay

bacterial colony counting

and live/dead staining all indicate that

CPMB not only inhibits biofilm formation of gram-negative

Pseudomonas aeruginosa

and

Acinetobacter baumannii

but also effectively disrupts mature biofilms that are highly resistant to one of the most active antibiotics—colistin. The superior antibacterial performance of the

CPMB implies its potential topical applications in treating biofilms.

关键词

Keywords

references

Parsek, M. R.; Singh, P. K . Bacterial biofilms: An emerging link to disease pathogenesis . Annu. Rev. Microbiol. , 2003 . 57 677 - 701 . DOI: 10.1146/annurev.micro.57.030502.090720 http://doi.org/10.1146/annurev.micro.57.030502.090720 .

Drescher, K.; Dunkeld, J.; Nadella, C. D.; Teeffelen, S. V.; Grnjaa, I.; Wingreenb, N. S.; Stone, H. A.; Bassler, B. L . Architectural transitions in Vibrio cholerae biofilms at single-cell resolution . Proc. Natl. Acad. Sci. , 2016 . 113 2066 - 2072 . DOI: 10.1073/pnas.1601702113 http://doi.org/10.1073/pnas.1601702113 .

Zheng, C. X.; Zhao, Y.; Liu, Y . Recent advances in self-assembled nano-therapeutics . Chinese J. Polym. Sci. , 2018 . 36 322 - 346 . .

Wei, T.; Yu, Q.; Chen, H . Responsive and synergistic antibacterial coatings: Fighting against bacteria in a smart and effective way . Adv. Healthc. Mater. , 2019 . 8 1801381 - 1801405 . DOI: 10.1002/adhm.v8.3 http://doi.org/10.1002/adhm.v8.3 .

Cheung, R. C. F.; Wong, J. H.; Pan, W. L.; Chan, Y. S.; Yin, C. M.; Dan, X. L.; Wang, H. X.; Fang, E. F.; Lam, S. K.; Ngai, P. H. K.; Xia, L. X.; Liu, F.; Ye, X. Y.; Zhang, G. Q.; Liu, Q. H.; Sha, O.; Lin, P.; Ki, C.; Bekhit, A. A.; Bekhit, A. E. D.; Wan, D. C. C.; Ye, X. J.; Xia, J.; Ng, T. B . Antifungal and antiviral products of marine organisms . Appl. Microbiol. Biotechnol. , 2014 . 98 3475 - 3494 . DOI: 10.1007/s00253-014-5575-0 http://doi.org/10.1007/s00253-014-5575-0 .

Kooa, H.; Allanb, R. N.; Howlind, R. P.; Hall-Stoodleye, L.; Stoodley, P . Targeting microbial biofilms: Current and prospective therapeutic strategies . Nat. Rev. Microbiol. , 2017 . 15 740 - 755 . DOI: 10.1038/nrmicro.2017.99 http://doi.org/10.1038/nrmicro.2017.99 .

Kim, S. K.; Lee, J. H . Biofilm dispersion in Pseudomonas aeruginosa . J. Microbiol. , 2016 . 54 71 - 85 . DOI: 10.1007/s12275-016-5528-7 http://doi.org/10.1007/s12275-016-5528-7 .

Jian, J. R.; Jie, Y. S.; Mei, T. L.; Ai, X. S.; Rong, X. Z.; Fang, L. S.; Hua, Y. J.; Quan, R. L.; Jie, Z . A biomimetic surface for infection-resistance through assembly of metal-phenolic networks . Chinese J. Polym. Sci. , 2018 . 36 576 - 583 . DOI: 10.1007/s10118-018-2032-z http://doi.org/10.1007/s10118-018-2032-z .

Vögeling, H.; Pinnapireddy, S. R.; Seitz, B.; Bakowsky, U . Indocyanine green loaded PLGA film coated coronary stents for photo-triggered in situ biofilm eradication . Colloid Interface Sci. Commun. , 2018 . 27 35 - 39 . DOI: 10.1016/j.colcom.2018.10.002 http://doi.org/10.1016/j.colcom.2018.10.002 .

Reffuveille, F.; Fuente-Núñez, C. D. L.; Mansour, S.; Hancock, R. E. W . A broad-spectrum antibiofilm peptide enhances antibiotic action against bacterial biofilms . Antimicrob. Agents Chemother. , 2014 . 58 5363 - 5371 . DOI: 10.1128/AAC.03163-14 http://doi.org/10.1128/AAC.03163-14 .

Uppu, D. S. S. M.; Konai, M. M.; Sarkar, P.; Samaddar, S.; Fensterseifer, I. C. M.; Farias-Junior, C.; Krishnamoorthy, P.; Shome, B. R.; Franco, O. V. L.; Haldar, J . Membrane-active macromolecules kill antibiotic-tolerant bacteria and potentiate antibiotics towards gram-negative bacteria . PLoS One , 2017 . 12 1 - 30 . .

Li, X. H.; Lee, J. H . Antibiofilm agents: A new perspective for antimicrobial strategy . J. Microbiol. , 2017 . 55 753 - 766 . DOI: 10.1007/s12275-017-7274-x http://doi.org/10.1007/s12275-017-7274-x .

Amato, S. M.; Fazen, C. H.; Henry, T. C.; Mok, W. K.; Orman, M. A.; Sandvik, E. L.; Volzing, K. G.; Brynildsen, M . The role of metabolism in bacterial persistence . Front. Microbiol. , 2014 . 5 1 - 9 . .

Balaban, N. Q.; Gerdes, K.; Lewis, K.; McKinney, J. D . A problem of persistence: Still more questions than answers? . Nat. Rev. Microbiol. , 2013 . 11 587 - 591 . .

Kumagai, Y.; Matsuo, J.; Cheng, Z.; Yoshihiro; Hayakawa; Rikihisa, Y . Cyclic dimeric GMP signaling regulates intracellular aggregation, sessility, and growth of Ehrlichia chaffeensis . Infect. Immun. , 2011 . 79 3905 - 3912 . DOI: 10.1128/IAI.05320-11 http://doi.org/10.1128/IAI.05320-11 .

Lewis, K . Persister cells . Annu. Rev. Microbiol. , 2010 . 64 357 - 372 . DOI: 10.1146/annurev.micro.112408.134306 http://doi.org/10.1146/annurev.micro.112408.134306 .

Balaban, N. Q.; Merrin, J.; Chait, R.; Kowalik, L.; Leibler, S . Bacterial persistence as a phenotypic switch . Science , 2004 . 305 1622 - 1626 . DOI: 10.1126/science.1099390 http://doi.org/10.1126/science.1099390 .

Jie, Z. Y.; Sheng, H. S.; Zhong, D. J . ε -Poly(L-lysine)-based hydrogels with fast-acting and prolonged antibacterial activities . Chinese J. Polym. Sci. , 2018 . 36 1239 - 1250 . DOI: 10.1007/s10118-018-2156-1 http://doi.org/10.1007/s10118-018-2156-1 .

Chua, S. L.; Yam, J. K. H.; Hao, P.; Adav, S. S.; Salido, M. M.; Liu, Y.; Givskov, M.; Sze, S. K.; Tolker-Nielsen, T.; Yang, L . Selective labelling and eradication of antibiotic-tolerant bacterial populations in Pseudomonas aeruginosa biofilms . Nat. Commun. , 2016 . 7 10750 - 10760 . DOI: 10.1038/ncomms10750 http://doi.org/10.1038/ncomms10750 .

Yang, Q.; Li, M.; Spiller, O. B.; Andrey, D. O.; Hinchliffe, P.; Li, H.; MacLean, C.; Niumsup, P.; Powell, L.; Pritchard, M.; Papkou, A.; Shen, Y.; Portal, E.; Sands, K.; Spencer, J.; Tansawai, U.; Thomas, D.; Wang, S.; Wang, Y.; Shen, J.; Walsh, T . Balancing mcr -1 expression and bacterial survival is a delicate equilibrium between essential cellular defence mechanisms . Nat. Commun. , 2017 . 8 2054 - 2066 . DOI: 10.1038/s41467-017-02149-0 http://doi.org/10.1038/s41467-017-02149-0 .

Zhao, D.; Xu, X. D.; Yuan, S. S.; Yan, S. J.; Wang, X. H.; Luan, S. F.; Yin, J. H . Fouling-resistant behavior of liquid-infused porous slippery surfaces . Chinese J. Polym. Sci. , 2017 . 35 887 - 896 . DOI: 10.1007/s10118-017-1930-9 http://doi.org/10.1007/s10118-017-1930-9 .

Wu, J.; Zhang, C.; Xu, S.; Pang, X.; Cai, G.; Wang, J . Preparation of zwitterionic polymer-functionalized cotton fabrics and the performance of anti-biofouling and long-term biofilm resistance . Colloid Interface Sci. Commun. , 2018 . 24 98 - 104 . DOI: 10.1016/j.colcom.2018.02.001 http://doi.org/10.1016/j.colcom.2018.02.001 .

Hancock, R. E. W.; Sahl, H. G . Antimicrobial and host-defense peptides as new anti-infective therapeutic strategies . Nat. Biotechnol. , 2006 . 24 1551 - 1557 . DOI: 10.1038/nbt1267 http://doi.org/10.1038/nbt1267 .

Nijnik, A.; Hancock, R. E. W . Host defence peptides: Antimicrobial and immunomodulatory activity and potential applications for tackling antibiotic-resistant infections . Emerg. Health Threats J. , 2009 . 2 ( e1 ): 1 - 7 . .

Yang, Y.; Cai, Z.; Huang, Z.; Tang, X.; Zhang, X . Antimicrobial cationic polymers: From structural design to functional control . Polym. J. , 2017 . 50 33 - 44 . .

Brogden, K. A . Antimicrobial peptides: Pore formers or metabolic inhibitors in bacteria? . Nat. Rev. Microbiol. , 2005 . 3 238 - 250 . .

Liu, S. P.; Zhou, L.; Lakshminarayanan, R.; Beuerman, R. W . Multivalent antimicrobial peptides as therapeutics: Design principles and structural diversities . Int. J. Pept. Res. Ther. , 2010 . 16 199 - 213 . DOI: 10.1007/s10989-010-9230-z http://doi.org/10.1007/s10989-010-9230-z .

Lam, S. J.; Wong, E. H. H.; O’Brien-Simpson, N. M.; Pantarat, N.; Blencowe, A.; Reynolds, E. C.; Qiao, G. G . Bionano interaction study on antimicrobial star-shaped peptide polymer nanoparticles . ACS Appl. Mater. Interfaces , 2016 . 8 33446 - 33456 . DOI: 10.1021/acsami.6b11402 http://doi.org/10.1021/acsami.6b11402 .

Cuthbert, T. J.; Hisey, B.; Harrison, T. D.; Trant, J. F.; Gillies, E. R.; Ragogna, P. J . Surprising antibacterial activity and selectivity of hydrophilic polyphosphoniums featuring sugar and hydroxy substituents . Angew. Chem. Int. Ed. , 2018 . 57 12707 - 12710 . DOI: 10.1002/anie.201806412 http://doi.org/10.1002/anie.201806412 .

Li, P.; Zhou, C.; Rayatpisheh, S.; Ye, K.; Poon, Y. F.; Hammond, P. T.; Duan, H.; Chan-Park, M. B . Cationic peptidopolysaccharides show excellent broad-spectrum antimicrobial activities and high selectivity . Adv. Mater. , 2012 . 24 4130 - 4137 . DOI: 10.1002/adma.201104186 http://doi.org/10.1002/adma.201104186 .

Ong, Z. Y.; Wiradharma, N.; Yang, Y. Y . Strategies employed in the design and optimization of synthetic antimicrobial peptide amphiphiles with enhanced therapeutic potentials . Adv. Drug Delivery Rev. , 2014 . 78 28 - 45 . DOI: 10.1016/j.addr.2014.10.013 http://doi.org/10.1016/j.addr.2014.10.013 .

Sang, P.; Shi, Y.; Teng, P.; Cao, A.; Xu, H.; Li, Q.; Cai, J . Antimicrobial AApeptides . Curr. Top. Med. Chem. , 2017 . 17 1266 - 1279 . DOI: 10.2174/1568026616666161018145945 http://doi.org/10.2174/1568026616666161018145945 .

Mowery, B. P.; Lee, S. E.; Kissounko, D. A.; Epand, R. F.; Epand, R. M.; Weisblum, B.; Stahl, S. S.; Gellman, S. H . Mimicry of antimicrobial host-defense peptides by random copolymers . J. Am. Chem. Soc. , 2007 . 129 15474 - 15476 . DOI: 10.1021/ja077288d http://doi.org/10.1021/ja077288d .

Yang. L. H.; Gordon.V. D.; Mishra. A.; Som. A.; Purdy K. R.; Davis. M. A.; Tew. G. N.; Gerard C. L. W . Synthetic antimicrobial oligomers induce a composition-dependent topological transition in membranes . J. Am. Chem. Soc. , 2007 . 129 12141 - 12147 . DOI: 10.1021/ja072310o http://doi.org/10.1021/ja072310o .

Lienkamp, K.; Madkour, A. E.; Musante, A.; Nelson, C. F.; sslein, K. N.; Tew, G. N . Antimicrobial polymers prepared by ROMP with unprecedented selectivity: A molecular construction kit approach . J. Am. Chem. Soc. , 2008 . 130 9836 - 9843 . DOI: 10.1021/ja801662y http://doi.org/10.1021/ja801662y .

Tew, G. N.; Scott, R. W.; Klein, M. L.; Degrado, W. F . De novo design of antimicrobial polymers, foldamers, and small molecules: From discovery to practical applications . Acc. Chem. Res. , 2010 . 43 30 - 39 . DOI: 10.1021/ar900036b http://doi.org/10.1021/ar900036b .

Liu, R.; Chen, X.; Falk, S. P.; Mowery, B. P.; Karlsson, A. J.; Weisblum, B.; Palecek, S. P.; Masters, K. S.; Gellman, S. H . Structure-activity relationships among antifungal Nylon-3 polymers: Identification of materials active against drug-resistant strains of Candida albicans . J. Am. Chem. Soc. , 2014 . 136 4333 - 4342 . DOI: 10.1021/ja500036r http://doi.org/10.1021/ja500036r .

Qian, Y.; Qi, F.; Chen, Q.; Zhang, Q.; Qiao, Z.; Zhang, S.; Wei, T.; Yu, Q.; Yu, S.; Mao, Z.; Gao, C.; Ding, Y.; Cheng, Y.; Jin, C.; Xie, H.; Liu, R . Surface modified with a host defense peptide-mimicking β -peptide polymer kills bacteria on contact with high efficacy . ACS Appl. Mater. Interfaces , 2018 . 10 15395 - 15400 . DOI: 10.1021/acsami.8b01117 http://doi.org/10.1021/acsami.8b01117 .

Zhang. D.; Qian. Y.; Zhang. S.; Ma. P.; Zhang. Q.; Shao. N.; Qi, F.; Xie. J.; Dai. C.; Zhou. R.; Qiao. Z.; Zhang. W.; Sheng, C.; Runhui, L . α - β Chimeric polypeptide molecular brushes display potent activity against superbugs-methicillin resistant Staphylococcus aureus . Sci. China Mater. , 2018 . 21 1 - 7 . .

Verduzco, R.; Li, X.; Peseka, S. L.; Stein, G. E . Structure, function, self-assembly, and applications of bottlebrush copolymers . Chem. Soc. Rev. , 2015 . 44 2405 - 2420 . DOI: 10.1039/C4CS00329B http://doi.org/10.1039/C4CS00329B .

Zhang, M.; Müller, A. H. E . Cylindrical polymer brushes . J. Polym. Sci., Part A: Polym. Chem. , 2005 . 43 3461 - 3481 . DOI: 10.1002/(ISSN)1099-0518 http://doi.org/10.1002/(ISSN)1099-0518 .

Sheiko, S. S.; Sumerlin, B. S.; Matyjaszewski, K . Cylindrical molecular brushes: Synthesis, characterization, and properties . Prog. Polym. Sci. , 2008 . 33 759 - 785 . DOI: 10.1016/j.progpolymsci.2008.05.001 http://doi.org/10.1016/j.progpolymsci.2008.05.001 .

Lu, X.; Tran, T. H.; Jia, F.; Tan, X.; Davis, S.; Krishnan, S.; Amiji, M. M.; Zhang, K . Providing oligonucleotides with steric selectivity by brush-polymer-assisted compaction . J. Am. Chem. Soc. , 2015 . 137 12466 - 12469 . DOI: 10.1021/jacs.5b08069 http://doi.org/10.1021/jacs.5b08069 .

Wang, J.; Lu, H.; Ren, Y.; Zhang, Y.; Morton, M.; Cheng, J.; Lin, Y . Interrupted helical structure of grafted polypeptides in brush-like macromolecules . Macromolecules , 2011 . 44 8699 - 8708 . DOI: 10.1021/ma201833b http://doi.org/10.1021/ma201833b .

Zhang, Y.; Yin, Q.; Lu, H.; Xia, H.; Lin, Y.; Cheng, J . PEG-polypeptide dual brush block copolymers: Synthesis and application in nanoparticle surface PEGylation . ACS Macro Lett. , 2013 . 2 809 - 813 . DOI: 10.1021/mz4003672 http://doi.org/10.1021/mz4003672 .

Li, Z . Synthesis of hetero-grafted amphiphilic diblock molecular brushes and their self-assembly in aqueous medium . Macromolecules , 2010 . 43 1182 - 1184 . DOI: 10.1021/ma902513n http://doi.org/10.1021/ma902513n .

Fan, J.; Borguet, Y. P.; Su, L.; Nguyen, T. P.; Wang, H.; He, X.; Zou, J.; Woole, K. L . Two-dimensional controlled syntheses of polypeptide molecular brushes via N -carboxyanhydride ring-opening polymerization and ring-opening metathesis polymerization . ACS Macro Lett. , 2017 . 6 1031 - 1035 . DOI: 10.1021/acsmacrolett.7b00603 http://doi.org/10.1021/acsmacrolett.7b00603 .

Gao, Q.; Yu, M.; Su, Y. J.; Xie, M. H.; Zhao, X.; Li, P.; Ma, P. X . Rationally designed dual functional block copolymers for bottlebrush-like coatings: In vitro and in vivo antimicrobial, antibiofilm, and antifouling properties . Acta Biomater. , 2017 . 51 112 - 124 . DOI: 10.1016/j.actbio.2017.01.061 http://doi.org/10.1016/j.actbio.2017.01.061 .

Elshaarawya, R. F. M.; Refaeec, A. A.; El-Sawi, E. A . Pharmacological performance of novel poly-(ionic liquid)-grafted chitosan- N -salicylidene Schiff bases and their complexes . Carbohydr. Polym. , 2016 . 146 376 - 387 . DOI: 10.1016/j.carbpol.2016.03.017 http://doi.org/10.1016/j.carbpol.2016.03.017 .

Rahman, M. A.; Bam, M.; Luat, E.; Jui, M. S.; Ganewatta, M. S.; Shokfai, T.; Nagarkatti, M.; Decho, A. W.; Tang, C . Macromolecular-clustered facial amphiphilic antimicrobials . Nat. Commun. , 2018 . 9 5231 - 5240 . DOI: 10.1038/s41467-018-07651-7 http://doi.org/10.1038/s41467-018-07651-7 .

Vincek, M. K.; Mor, A.; Gorgieva, S.; Kokol, V . Antibacterial activity and cytotoxycity of gelatine-conjugated lysine-based peptides . J. Biomed. Mater. Res., Part A , 2017 . 105 3110 - 3126 . DOI: 10.1002/jbm.a.36164 http://doi.org/10.1002/jbm.a.36164 .

Yao, D.; Guo, Y.; Chen, S.; Tang, J.; Chen, Y . Shaped core/shell polymer nanoobjects with high antibacterial activities via block copolymer microphase separation . Polymer , 2013 . 54 3485 - 3491 . DOI: 10.1016/j.polymer.2013.05.005 http://doi.org/10.1016/j.polymer.2013.05.005 .

Yang, C.; Ding, X.; Ono, R. J.; Lee, H.; Hsu, L. Y.; Tong, Y. W.; Hedrick, J.; Yang, Y. Y . Brush-like polycarbonates containing dopamine, cations, and PEG providing a broad-spectrum, antibacterial, and antifouling surface via one-step coating . Adv. Mater. , 2014 . 26 7346 - 7351 . DOI: 10.1002/adma.v26.43 http://doi.org/10.1002/adma.v26.43 .

Mohamed, M. F.; Brezden, A.; Mohammad, H.; Chmielewski, J.; Seleem, M. N . A short D-enantiomeric antimicrobial peptide with potent immunomodulatory and antibiofilm activity against multidrug-resistant Pseudomonas aeruginosa and Acinetobacter baumannii . Sci. Rep. , 2017 . 7 6953 DOI: 10.1038/s41598-017-07440-0 http://doi.org/10.1038/s41598-017-07440-0 .

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Biofilm Disruption Utilizing α/β Chimeric Polypeptide Molecular Brushes

DOI：10.1007/s10118-019-2278-0

摘要

Abstract

关键词

Keywords

references