Controllable Emission <italic style="font-style: italic">via</italic> Tuning the Size of Fluorescent Nano-probes Formed by Polymeric Amphiphiles

Xiao-Cheng Wang; Shi-Xin Zhou; Lan Ding; Yu-Han Zhao; Shen-Xi Min; Bin Dong; Bo Song

doi:10.1007/s10118-019-2256-6

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Controllable Emission via Tuning the Size of Fluorescent Nano-probes Formed by Polymeric Amphiphiles

ARTICLE | Updated：2021-02-18

- Controllable Emission via Tuning the Size of Fluorescent Nano-probes Formed by Polymeric Amphiphiles
- Controllable Emission via Tuning the Size of Fluorescent Nano-probes Formed by Polymeric Amphiphiles
- Chinese Journal of Polymer Science 2019年37卷第8期页码：767-773
- Affiliations：
  
  a.College of Chemistry, Chemical Engineering and Materials Science, Soochow University, Suzhou 215123, China
  b.Department of Cell Biology and Stem Cell Research Center, School of Basic Medical Sciences, Peking University Health Science Center, Beijing 100191, China
  c.Institute of Functional Nano & Soft Materials (FUNSOM) & Collaborative Innovation Center for Suzhou Nano Science and Technology, Jiangsu Key Laboratory for Carbon-Based Functional Materials & Devices, Soochow University, Suzhou 215123, China
- Author bio：
  
  songbo@suda.edu.cn
- Funds：
  
  This work was financially supported by the National Natural Science Foundation of China (Nos. 21674075 and 21233003), the Natural Science Foundation of Jiangsu Province (No. BK20161211), Key University Science Research Project of Jiangsu Province (No. 17KJA150007), and a Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions.
- DOI：10.1007/s10118-019-2256-6
  中图分类号：
- 收稿：2019-01-28，
  
  修回：2019-1-1，
  
  录用：2019-3-18，
  
  网络首发：2019-04-24，
  
  纸质出版：2019-08
- Accepted：
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Citation: Wang, X. C.; Zhou, S. X.; Ding, L.; Zhao, Y. H.; Min, S. X.; Dong, B.; Song, B. Controllable emission via tuning the size of fluorescent nano-probes formed by polymeric amphiphiles. Chinese J. Polym. Sci. 2019, 37, 767–773

Xiao-Cheng Wang, Shi-Xin Zhou, Lan Ding, et al. Controllable Emission via Tuning the Size of Fluorescent Nano-probes Formed by Polymeric Amphiphiles[J]. Chinese Journal of Polymer Science, 2019, 37(8): 767-773. DOI: 10.1007/s10118-019-2256-6.
Citation: Wang, X. C.; Zhou, S. X.; Ding, L.; Zhao, Y. H.; Min, S. X.; Dong, B.; Song, B. Controllable emission via tuning the size of fluorescent nano-probes formed by polymeric amphiphiles. Chinese J. Polym. Sci. 2019, 37, 767–773 DOI： 10.1007/s10118-019-2256-6.

Xiao-Cheng Wang, Shi-Xin Zhou, Lan Ding, et al. Controllable Emission via Tuning the Size of Fluorescent Nano-probes Formed by Polymeric Amphiphiles[J]. Chinese Journal of Polymer Science, 2019, 37(8): 767-773. DOI: 10.1007/s10118-019-2256-6. DOI：

摘要

Abstract

Incorporating fluorophores into polymeric nanoparticles has been testified as a feasible way to improve the emitting property and bio-compatibility of nano-emitters

which can be applied as fluorescent probes in labeling cells for imaging. Plenty of efforts have been made on the above direction. However

the size effect of nano-emitters has not been addressed yet mainly given the difficulties in controlling morphology and size of the assemblies. In our preceding study

we employed post-polymerization modification method for preparing amphiphilic copolymers

and obtained core-shell (the hydrophobic fluorophores are wrapped inside the nanoparticle to form the core) assemblies in aqueous solution. By this method

we are able to regulate the ratio of the hydrophilic/hydrophobic moieties

and thus alternate the size of the assemblies in a rather simple way. In this study

we synthesized a series of random copolymers by changing the ratio of poly(ethylene glycol) to tetraphenylethylene groups. Notably

the number of repeating units of the polymer was controlled constant for all the copolymers. The self-assembly of these copolymers resulted in different sizes of nanoparticles

and the size decreased with the decreasing fraction of poly(ethylene glycol). Interestingly

the emission of the nanoparticles showed size dependence

and smaller diameter corresponded to stronger emission. Being cultured with HeLa cells

either the large (diameter of ~300 nm) or the small (diameter of ~180 nm) nano-emitters allowed for very high cell viabilities up to 25 μg·mL

−1

. Both of them can be applied in cell imaging and provide high contrast fluorescent images.

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Controllable Emission via Tuning the Size of Fluorescent Nano-probes Formed by Polymeric Amphiphiles

DOI：10.1007/s10118-019-2256-6

摘要

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