Reversible Protein Adsorption on PMOXA/PAA Based Coatings: Role of PAA

Fatima Mumtaz; Chao-Shi Chen; Hai-Kun Zhu; Muhammad Atif; Yan-Mei Wang

doi:10.1007/s10118-018-2168-x

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Reversible Protein Adsorption on PMOXA/PAA Based Coatings: Role of PAA

ARTICLE | Updated：2021-02-18

- Reversible Protein Adsorption on PMOXA/PAA Based Coatings: Role of PAA
- Reversible Protein Adsorption on PMOXA/PAA Based Coatings: Role of PAA
- Chinese Journal of Polymer Science 2018年36卷第12期页码：1328-1341
- Affiliations：
  
  Chinese Academy of Sciences Key Laboratory of Soft Matter Chemistry, Department of Polymer Science and Engineering, University of Science and Technology of China, Hefei 230026, China
- Author bio：
  
  wangyanm@ustc.edu.cn
- Funds：
  
  This work was financially supported by the National Natural Science Foundation of China (No. 21674102). We gratefully acknowledge to Prof. Guangming Liu and Dr. Xiaodong Ye (Department of Chemical Physics, University of Science and Technology of China, Hefei, China) for QCM, ellipsometry and GPC analysis.
- DOI：10.1007/s10118-018-2168-x
  中图分类号：
- 收稿：2018-05-10，
  
  修回：2018-5-25，
  
  录用：2018-5-28，
  
  网络首发：2018-07-04，
  
  纸质出版：2018-12
- Accepted：
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Citation: Mumtaz, F.; Chen, C. S.; Zhu, H. K.; Atif, M.; Wang, Y. M. Reversible Protein Adsorption on PMOXA/PAA Based Coatings: Role of PAA. Chinese J. Polym. Sci. 2018, 36(12), 1328–1341

Fatima Mumtaz, Chao-Shi Chen, Hai-Kun Zhu, et al. Reversible Protein Adsorption on PMOXA/PAA Based Coatings: Role of PAA[J]. Chinese Journal of Polymer Science, 2018, 36(12): 1328-1341.
Citation: Mumtaz, F.; Chen, C. S.; Zhu, H. K.; Atif, M.; Wang, Y. M. Reversible Protein Adsorption on PMOXA/PAA Based Coatings: Role of PAA. Chinese J. Polym. Sci. 2018, 36(12), 1328–1341 DOI： 10.1007/s10118-018-2168-x.

Fatima Mumtaz, Chao-Shi Chen, Hai-Kun Zhu, et al. Reversible Protein Adsorption on PMOXA/PAA Based Coatings: Role of PAA[J]. Chinese Journal of Polymer Science, 2018, 36(12): 1328-1341. DOI： 10.1007/s10118-018-2168-x.

摘要

Abstract

In this study we report design of stimuli-responsive coating based on poly(2-methyl-2-oxazoline-random-glycidyl methacrylate) (PMOXA-

-GMA) comb copolymer and poly(acrylic acid)-block-poly(glycidyl methacrylate) (PAA-

-PGMA) block copolymers and scrutinize its ability to control protein adsorption. Firstly

PMOXA/PAA based coatings were prepared by simply spin coating the mixture of PMOXA-

-GMA and PAA-

-PGMA copolymer solutions onto silicon substrates followed by annealing at 110 °C. Then coatings were rigorously characterized by X-ray photoelectron spectroscopy (XPS)

the static water contact angle (WCA) test

ellipsometry and atomic force microscopy (AFM). After that

the relationship of switchable behavior of PMOXA/PAA based coatings with PAA content and chain length was investigated through the change of thickness and WCA upon pH and ionic strength (

) trigger

which indicated that the change in thickness and WCA was triggered when PAA contents were increased as well as by increasing chain length of PAA in PMOXA/PAA based coatings. Finally

real-time adsorption/desorption of lysozyme (Lyso) on PMOXA/PAA based coatings was monitored using quartz crystal microbalance with dissipation monitoring (QCM-D). The results showed that the Lyso adsorption amount was increased upon increasing chain length and contents of PAA in PMOXA/PAA based coatings. The adsorbed Lyso was then efficiently desorbed by changing pH and

of medium with the maximum desorption (

90% desorption percentage) observed for the suitable ratio of PMOXA and PAA while chain length of PAA was kept longer than that of PMOXA. Furthermore

the prepared coatings were found to repeatedly adsorb and desorb Lyso for four successive cycles of adsorption/desorption

which confirmed the stability of prepared coatings.

关键词

Keywords

references

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