Co-delivery of Andrographolide and Notch1-targeted siRNA to Macrophages with Polymer-based Nanocarrier for Enhanced Anti-inflammation

Teng Wu; Min Tan; Heng-Ye Gong; Yong Wang; Xin-Tao Shuai

doi:10.1007/s10118-018-2158-z

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Co-delivery of Andrographolide and Notch1-targeted siRNA to Macrophages with Polymer-based Nanocarrier for Enhanced Anti-inflammation

ARTICLE | Updated：2021-02-18

- Co-delivery of Andrographolide and Notch1-targeted siRNA to Macrophages with Polymer-based Nanocarrier for Enhanced Anti-inflammation
- Co-delivery of Andrographolide and Notch1-targeted siRNA to Macrophages with Polymer-based Nanocarrier for Enhanced Anti-inflammation
- Chinese Journal of Polymer Science 2018年36卷第12期页码：1312-1320
- Affiliations：
  
  a.PCFM Lab of Ministry of Education, School of Materials Science and Engineering, Sun Yat-Sen University, Guangzhou 510275, China
  b.Center of Biomedical Engineering, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou 510080, China
- Author bio：
  
  shuaixt@mail.sysu.edu.cn
- Funds：
  
  This work was financially supported by the National Natural Science Foundation of China (No. U1401242), National Basic Research Program of China (No. 2015CB755500), the Guangdong Innovative and Entrepreneurial Research Team Program (No. 2013S086) and the Fundamental Research Funds for the Central Universities (Nos. 17lgjc01 and 17lgpy08).
- DOI：10.1007/s10118-018-2158-z
  中图分类号：
- 收稿：2018-04-25，
  
  修回：2018-5-9，
  
  录用：2018-5-10，
  
  网络首发：2018-06-22，
  
  纸质出版：2018-12
- Accepted：
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Citation: Wu, T.; Tan, M.; Gong, H. Y.; Wang, Y.; Shuai, X. T. Co-delivery of Andrographolide and Notch1-targeted siRNA to Macrophages with Polymer-based Nanocarrier for Enhanced Anti-inflammation. Chinese J. Polym. Sci. 2018, 36(12), 1312–1320

Teng Wu, Min Tan, Heng-Ye Gong, et al. Co-delivery of Andrographolide and Notch1-targeted siRNA to Macrophages with Polymer-based Nanocarrier for Enhanced Anti-inflammation[J]. Chinese Journal of Polymer Science, 2018, 36(12): 1312-1320.
Citation: Wu, T.; Tan, M.; Gong, H. Y.; Wang, Y.; Shuai, X. T. Co-delivery of Andrographolide and Notch1-targeted siRNA to Macrophages with Polymer-based Nanocarrier for Enhanced Anti-inflammation. Chinese J. Polym. Sci. 2018, 36(12), 1312–1320 DOI： 10.1007/s10118-018-2158-z.

Teng Wu, Min Tan, Heng-Ye Gong, et al. Co-delivery of Andrographolide and Notch1-targeted siRNA to Macrophages with Polymer-based Nanocarrier for Enhanced Anti-inflammation[J]. Chinese Journal of Polymer Science, 2018, 36(12): 1312-1320. DOI： 10.1007/s10118-018-2158-z.

摘要

Abstract

Chronic inflammatory responses induced by macrophages play a pivotal role in the progression of atherosclerosis. In the present study

a multifunctional nanocarrier based on poly(ethylene glycol)-block-poly(L-aspartic acid) grafted with diethylenetriamine

lysine and cholic acid (PEG-PAsp(DETA)-Lys-CA

) polymer was synthesized for co-delivery of andrographolide and siRNA targeting Notch1 gene to alleviate the inflammatory response in macrophages. The nanocarrier exerted low cytotoxicity as well as high performance in drug/siRNA co-delivery.

In vitro

studies demonstrated the co-delivery of andrographolide and Notch1 siRNA not only significantly inhibited lipopolysaccharide (LPS)-activated interleukin-6 (IL-6) and monocytes chemotactic protein 1 (MCP-1) expression as well as blocked nuclear factor-κB (NF-κB) signal activation

but also interfered the Notch1 gene expression and increased anti-inflammatory cytokines such as interleukin-10 (IL-10) and arginase-1 expression obviously in macrophages. These results suggested that the combination therapy based on Notch1 siRNA and andrographolide co-delivered nanocarrier

i.e.

suppressing the expression of pro-inflammatory cytokines while simultaneously increasing anti-inflammatory factors expression

be a feasible strategy for atherosclerosis treatment.

关键词

Keywords

references

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