Oriented Antibody Immobilization and Immunoassay Based on Boronic Acid-containing Polymer Brush

Jie Zhao; Ru Mo; Li-Mei Tian; Ling-Jie Song; Shi-Fang Luan; Jing-Hua Yin; Lu-Quan Ren

doi:10.1007/s10118-018-2031-0

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Oriented Antibody Immobilization and Immunoassay Based on Boronic Acid-containing Polymer Brush

Articles | Updated：2021-02-18

- Oriented Antibody Immobilization and Immunoassay Based on Boronic Acid-containing Polymer Brush
- Oriented Antibody Immobilization and Immunoassay Based on Boronic Acid-containing Polymer Brush
- Chinese Journal of Polymer Science 2018年36卷第4期页码：472-478
- Affiliations：
  
  a.Key Laboratory of Bionic Engineering, Ministry of Education, Jilin University, Changchun 130022, China
  b.State Key Laboratory of Polymer Physics and Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China
  c.Jilin Province People's Hospital, Changchun 130021, China
- Author bio：
  
  Jie Zhao, E-mail jiezhao@jlu.edu.cn
- Funds：
- DOI：10.1007/s10118-018-2031-0
  中图分类号：
- 收稿：2017-08-23，
  
  录用：2017-8-30，
  
  网络首发：2017-12-01，
  
  纸质出版：2018-04-05
- Accepted：
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Jie Zhao, Ru Mo, Li-Mei Tian, 等. Oriented Antibody Immobilization and Immunoassay Based on Boronic Acid-containing Polymer Brush[J]. Chinese Journal of Polymer Science, 2018,36(4):472-478.

Jie Zhao, Ru Mo, Li-Mei Tian, et al. Oriented Antibody Immobilization and Immunoassay Based on Boronic Acid-containing Polymer Brush[J]. Chinese Journal of Polymer Science, 2018, 36(4): 472-478.
Jie Zhao, Ru Mo, Li-Mei Tian, 等. Oriented Antibody Immobilization and Immunoassay Based on Boronic Acid-containing Polymer Brush[J]. Chinese Journal of Polymer Science, 2018,36(4):472-478. DOI： 10.1007/s10118-018-2031-0.

Jie Zhao, Ru Mo, Li-Mei Tian, et al. Oriented Antibody Immobilization and Immunoassay Based on Boronic Acid-containing Polymer Brush[J]. Chinese Journal of Polymer Science, 2018, 36(4): 472-478. DOI： 10.1007/s10118-018-2031-0.

摘要

Abstract

High sensitive immunoassay platforms have gained intense attention due to their vital roles in early-stage disease diagnosis and therapeutic information feedback. Although random covalent-binding of antibody has been widely adopted in immunoassays due to its simplicity and effectiveness

it readily loses its activity and fails to exhibit high antigen-binding capacity. In this work

copolymer of zwitterionic sulfobetaine methacrylate (SBMA) and glycidyl methacrylate (GMA) brushes were immobilized onto inert polypropylene (PP)

via

surface-initiated atom transfer radical polymerization (ATRP) based on biomimetic dopamine pretreatment. Subsequently

boronic acid (BA) groups were covalently bonded

via

active GMA units

followed by the introduction of oriented immobilization of antibody. Owing to the oriented immobilization of antibody facilitated by BA groups in polymer brush

the bioactivity of antibody is well preserved

which endows the surface with significantly enhanced antigen-binding capacity. Moreover

the existence of SBMA segments in polymer brushes renders the surface high resistance to nonspecific protein adsorption

significantly alleviating the signal interference of antigen recognition. This strategy could find potential applications in developing high sensitive immunoassay platforms based on the different substrates.

关键词

Keywords

references

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