Affinity Switching for Lysozyme and Dual-responsive Microgels by Stopped-flow Technique:Kinetic Control and Activity Evaluation

Yun-zhu Wu; Zhi-huang Zhang; Xin Han; Jian Zhang; Wen-ming Zhang; Jun Yin

doi:10.1007/s10118-017-1948-z

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Affinity Switching for Lysozyme and Dual-responsive Microgels by Stopped-flow Technique:Kinetic Control and Activity Evaluation

Papers | Updated：2021-02-18

- Affinity Switching for Lysozyme and Dual-responsive Microgels by Stopped-flow Technique:Kinetic Control and Activity Evaluation
- Affinity Switching for Lysozyme and Dual-responsive Microgels by Stopped-flow Technique:Kinetic Control and Activity Evaluation
- Chinese Journal of Polymer Science 2017年35卷第8期页码：950-960
- Affiliations：
  
  Department of Polymer Science and Engineering, School of Chemistry and Chemical Engineering, Hefei University of Technology and Anhui Key Laboratory of Advanced Functional Materials and Devices, Hefei 230009, China
- Author bio：
  
  Jun Yin (殷俊),E-mail:yinjun@hfut.edu.cn
- Funds：
  
  the National Natural Science Foundation of China(51673058);Supplementary material is available in the online version of this article at http://dx.doi.org/10.1007/s10118-017-1948-z.
- DOI：10.1007/s10118-017-1948-z
  中图分类号：
- 收稿：2017-02-09，
  
  修回：2017-02-22，
  
  录用：2017-3-7，
  
  纸质出版：2017-08
- Accepted：
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Yun-zhu Wu, Zhi-huang Zhang, Xin Han, 等. Affinity Switching for Lysozyme and Dual-responsive Microgels by Stopped-flow Technique:Kinetic Control and Activity Evaluation[J]. Chinese Journal of Polymer Science, 2017,35(8):950-960.

Yun-zhu Wu, Zhi-huang Zhang, Xin Han, et al. Affinity Switching for Lysozyme and Dual-responsive Microgels by Stopped-flow Technique:Kinetic Control and Activity Evaluation[J]. Chinese Journal of Polymer Science, 2017, 35(8): 950-960.
Yun-zhu Wu, Zhi-huang Zhang, Xin Han, 等. Affinity Switching for Lysozyme and Dual-responsive Microgels by Stopped-flow Technique:Kinetic Control and Activity Evaluation[J]. Chinese Journal of Polymer Science, 2017,35(8):950-960. DOI： 10.1007/s10118-017-1948-z.

Yun-zhu Wu, Zhi-huang Zhang, Xin Han, et al. Affinity Switching for Lysozyme and Dual-responsive Microgels by Stopped-flow Technique:Kinetic Control and Activity Evaluation[J]. Chinese Journal of Polymer Science, 2017, 35(8): 950-960. DOI： 10.1007/s10118-017-1948-z.

摘要

Abstract

The use of proteins as therapeutics in nanomedicine is an emerging research field and has developed rapidly. However

proteins are always vulnerable to renal excretion or digestion by the proteolytic system

in vivo

which limits their usage to a large extent. Although biocompatible polymers have been covalently linked to proteins to protect them from recognition by the immune system and prolong their circulation time

the biological activity of them is sometimes decreased. To fill this gap

physical isolation

wrapping

or encapsulation techniques are employed. Up to now

various mature examples were reported

but the whole time scales for guest molecules loading and releasing

especially the initial rapid loading process

were rarely mentioned. Herein

a series of dual-responsive poly(

-isopropylacrylamide-

-methacrylic acid) (P(NIPAM-

-MAA)) microgels were synthesized and employed to investigate the kinetics of

in situ

complexation and release of lysozyme under external stimuli modulation upon a stopped-flow apparatus

which was suitable for rapid dynamic monitoring. Close inspection of the adsorption kinetics during the early stages (

50 s) revealed that the initial microgel collapse occurred within~1 s

with more rapid transitions being observed when higher lysozyme concentrations were targeted. All the dynamic traces could be well fitted with a double exponential function

suggesting a fast (

) and a slow (

) relaxation time

respectively. Then

the kinetics of releasing bound lysozyme from microgels was carried on by utilizing the pH-responsive property

and the evaluation of the activity of released lysozyme was synchronously measured in a Micrococcus lysodeikticus (

M. lysodeikticus

) cell suspension. The corresponding relaxation time (

) was also calculated by fitting the recorded dynamic traces. We speculate that this work can provide basic dynamics data and theoretical basis for microgels based nanocarriers to be used for protein delivery

controlled release

and possible chemical separation.

关键词

Keywords

references

T. Oya , T. Enoki , A.Y. Grosberg , S. Masamune , T. Sakiyama , Y. Takeoka , K. Tanaka , G.Q. Wang , Y. Yilmaz , M.S. Feld , R. Dasari , T. Tanaka . . Science , 1999 . 286 1543 DOI: 10.1126/science.286.5444.1543 http://doi.org/10.1126/science.286.5444.1543 .

K. Miyata , Y. Kakizawa , N. Nishiyama , A. Harada , Y. Yamasaki , H. Koyama , K. Kataoka . . J. Am. Chem. Soc. , 2004 . 126 2355 DOI: 10.1021/ja0379666 http://doi.org/10.1021/ja0379666 .

K. Yoshimatsu , B.K. Lesel , Y. Yonamine , J.M. Beierle , Y. Hoshino , K.J. Shea . . Angew. Chem. Int. Ed. , 2012 . 124 2455 DOI: 10.1002/ange.v124.10 http://doi.org/10.1002/ange.v124.10 .

Z.S. Ge , S.Y. Liu . . Chem. Soc. Rev. , 2013 . 42 7289 DOI: 10.1039/c3cs60048c http://doi.org/10.1039/c3cs60048c .

Z.Y. Deng , Y.F. Qian , Y.Q. Yu , G.H. Liu , J.M. Hu , G.Y. Zhang , S.Y. Liu . . J. Am. Chem. Soc. , 2016 . 138 10452 DOI: 10.1021/jacs.6b04115 http://doi.org/10.1021/jacs.6b04115 .

Z.B. Li , Y.H. Xiang , X.J. Zhou , J.J. Nie , M. Peng , B.Y. Du . . Chinese J. Polym. Sci. , 2015 . 33 ( 11 ): 1516 DOI: 10.1007/s10118-015-1694-z http://doi.org/10.1007/s10118-015-1694-z .

J.J. Liu , Y.F. Yan , P. Yao . . Chinese J. Polym. Sci. , 2011 . 29 ( 4 ): 397 DOI: 10.1007/s10118-011-1054-6 http://doi.org/10.1007/s10118-011-1054-6 .

S.Y. Xing , Y. Guan , Y.J. Zhang . . Macromolecules , 2011 . 44 4479 DOI: 10.1021/ma200586w http://doi.org/10.1021/ma200586w .

N. Huang , Y. Guan , X.X. Zhu , Y.J. Zhang . . Chem. Phys. Chem. , 2014 . 15 1785 DOI: 10.1002/cphc.v15.9 http://doi.org/10.1002/cphc.v15.9 .

L.H. Li , Y. Zhu , B. Li , C.Y. Gao . . Langmuir , 2008 . 24 13632 DOI: 10.1021/la802556e http://doi.org/10.1021/la802556e .

Y.P. Li , W.W. Xiao , K. Xiao , L. Berti , J. Luo , H.P. Tseng , G. Fung , K.S. Lam . . Angew. Chem. Int. Ed. , 2012 . 124 2918 DOI: 10.1002/ange.201107144 http://doi.org/10.1002/ange.201107144 .

F.H. Meng , Y.L. Li , L. Zhu , Z.Z. Liu , R. Cheng , J.H. Cui , S.J. Ji , Z.Y. Zhong . . Angew. Chem. Int. Ed. , 2009 . 48 9914 DOI: 10.1002/anie.v48:52 http://doi.org/10.1002/anie.v48:52 .

H. Zhang , S. Mardyani , W.C.W. Chan , E. Kumacheva . . Biomacromolecules , 2006 . 7 1568 DOI: 10.1021/bm050912z http://doi.org/10.1021/bm050912z .

M.J. Roberts , M.D. Bentley , J.M. Harris . . Adv. Drug Delivery Rev. , 2002 . 54 459 DOI: 10.1016/S0169-409X(02)00022-4 http://doi.org/10.1016/S0169-409X(02)00022-4 .

F. Cartei , G. Cartei , V. Ceschia , S. Pacor , G. Sava . . Curr. Therap. Res. Clin. Exp. , 1991 . 50 530 .

L. Tao , G. Mantovani , F. Lecolley , D.M. Haddleton . . J. Am. Chem. Soc. , 2004 . 126 13220 DOI: 10.1021/ja0456454 http://doi.org/10.1021/ja0456454 .

D. Bontempo , H.D. Maynard . . J. Am. Chem. Soc. , 2005 . 127 6508 DOI: 10.1021/ja042230+ http://doi.org/10.1021/ja042230+ .

C. Boyer , V. Bulmus , J.Q. Liu , T.P. Davis , M.H. Stenzel , C. Barner-Kowollik . . J. Am. Chem. Soc. , 2007 . 129 7145 DOI: 10.1021/ja070956a http://doi.org/10.1021/ja070956a .

K.L. Heredia , D. Bontempo , T. Ly , J.T. Byers , S. Halstenberg , H.D. Maynard . . J. Am. Chem. Soc. , 2005 . 127 16955 DOI: 10.1021/ja054482w http://doi.org/10.1021/ja054482w .

L. Tao , J.Q. Liu , J.T. Xu , T.P. Davis . . Org. Biomol. Chem. , 2009 . 7 3481 DOI: 10.1039/b907061c http://doi.org/10.1039/b907061c .

G.G. Kochendoerfer , S.Y. Chen , F. Mao , S. Cressman , S. Traviglia , H. Shao , C.L. Hunter , D.W. Low , E.N. Cagle , M. Carnevali , V. Gueriguian , P.J. Keogh , H. Porter , S.M. Stratton , M.C. Wiedeke , J. Wilken , J. Tang , J.J. Levy , L.P. Miranda , M.M. Crnogorac , S. Kalbag , P. Botti , J. Schindler-Horvat , L. Savatski , J.W. Adamson , A. Kung , S.B.H. Kent , J.A. Bradburne . . Science , 2003 . 299 884 DOI: 10.1126/science.1079085 http://doi.org/10.1126/science.1079085 .

S. Lindhoud , L. Voorhaar , R.D. Vries , R. Schweins , M.A.C. Stuart , W. Norde . . Langmuir , 2009 . 25 11425 DOI: 10.1021/la901591p http://doi.org/10.1021/la901591p .

S. Ganguli , K. Yoshimoto , S. Tomita , H. Sakuma , T. Matsuoka , K. Shiraki , Y. Nagasaki . . J. Am. Chem. Soc. , 2009 . 131 6549 DOI: 10.1021/ja900786z http://doi.org/10.1021/ja900786z .

M. Danial , H. Klok , W. Norde , M.A.C. Stuart . . Langmuir , 2007 . 23 8003 DOI: 10.1021/la700573j http://doi.org/10.1021/la700573j .

J. Gummel , F. Boue , B. Deme , F. Cousin . . J. Phys. Chem. B , 2006 . 110 24837 DOI: 10.1021/jp064383k http://doi.org/10.1021/jp064383k .

A. Harada , K. Kataoka . . Macromolecules , 1998 . 31 288 DOI: 10.1021/ma971277v http://doi.org/10.1021/ma971277v .

S. Oncel , L. Uzun , B. Garipcan , A. Denizli . . Ind. Eng. Chem. Res. , 2005 . 44 7049 DOI: 10.1021/ie0506318 http://doi.org/10.1021/ie0506318 .

J.M. Beierle , K. Yoshimatsu , B. Chou , M.A.A. Mathews , B.K. Lesel , K.J. Shea . . Angew. Chem. Int. Ed. , 2014 . 53 9275 DOI: 10.1002/anie.201404881 http://doi.org/10.1002/anie.201404881 .

D. Dupin , J. Rosselgong , S.P. Armes , A. Routh . . Langmuir , 2007 . 23 4035 DOI: 10.1021/la063278z http://doi.org/10.1021/la063278z .

J. Yin , D. Dupin , J.F. Li , S.P. Armes , S.Y. Liu . . Langmuir , 2008 . 24 9334 DOI: 10.1021/la8014282 http://doi.org/10.1021/la8014282 .

T. Tanaka , D.J. Fillmore . . J. Chem. Phys. , 1979 . 70 1214 DOI: 10.1063/1.437602 http://doi.org/10.1063/1.437602 .

D.V. Subotic , X.Y. Wu . . Ind. Eng. Chem. Res. , 1997 . 36 1302 DOI: 10.1021/ie9604987 http://doi.org/10.1021/ie9604987 .

S.Q. Zhou , B. Chu . . J. Phys. Chem. B , 1998 . 102 1364 DOI: 10.1021/jp972990p http://doi.org/10.1021/jp972990p .

D. Shugar . . Biochim. Biophys. Acta , 1952 . 8 302 DOI: 10.1016/0006-3002(52)90045-0 http://doi.org/10.1016/0006-3002(52)90045-0 .

J. Yin , S.Y. Shi , J.M. Hu , S.Y. Liu . . Langmuir , 2014 . 30 9551 DOI: 10.1021/la501918s http://doi.org/10.1021/la501918s .

J. Yin , X.F. Guan , D. Wang , S.Y. Liu . . Langmuir , 2009 . 25 11367 DOI: 10.1021/la901377h http://doi.org/10.1021/la901377h .

F.A. Plamper , H. Becker , M. Lanzendorfer , M. Patel , A. Wittemann , M. Ballauf , A.H.E. Muller . . Macromol. Chem. Phys. , 2005 . 206 1813 DOI: 10.1002/(ISSN)1521-3935 http://doi.org/10.1002/(ISSN)1521-3935 .

A.F. Olea , J.K. Thomas . . Macromolecules , 1989 . 22 1165 DOI: 10.1021/ma00193a029 http://doi.org/10.1021/ma00193a029 .

M. Shibayama , K. Nagai . . Macromolecules , 1999 . 32 7461 DOI: 10.1021/ma990719v http://doi.org/10.1021/ma990719v .

D. Nakimbugwe , B. Masschalck , D. Deckers , L. Callewaert , A. Aertsen , C.W. Michiels . . FEMS Microbiol. Lett. , 2006 . 259 41 DOI: 10.1111/fml.2006.259.issue-1 http://doi.org/10.1111/fml.2006.259.issue-1 .

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