Thermo-sensitive Poly(DEGMMA-co-MEA) Microgels: Synthesis, Characterization and Interfacial Interaction with Adsorbed Protein Layer

Zhen-bing Li; Yan-hui Xiang; Xian-jing Zhou; Jing-jing Nie; Mao Peng; Bin-yang Du

doi:10.1007/s10118-015-1694-z

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Thermo-sensitive Poly(DEGMMA-co-MEA) Microgels: Synthesis, Characterization and Interfacial Interaction with Adsorbed Protein Layer

PAPER | Updated：2021-06-21

- Thermo-sensitive Poly(DEGMMA-co-MEA) Microgels: Synthesis, Characterization and Interfacial Interaction with Adsorbed Protein Layer
- Thermo-sensitive Poly(DEGMMA-co-MEA) Microgels: Synthesis, Characterization and Interfacial Interaction with Adsorbed Protein Layer
- Chinese Journal of Polymer Science 2015年33卷第11期页码：1516-1526
- Affiliations：
  
  a.MOE Key Laboratory of Macromolecular Synthesis and Functionalization, Department of Polymer Science & Engineering, Zhejiang University, Hangzhou 310027, China
  b.Department of Chemistry, Zhejiang University, Hangzhou 310027, China
- Author bio：
  
  **Bin-yang Du (杜滨阳), E-mail: duby@zju.edu.cn
- Funds：
  
  National Natural Science Foundation of China(21274129;21322406);Fundamental Research Funds for the Central Universities(2014XZZX003-21);third level of 2013 Zhejiang Province 151 Talent Project, and Open Research Fund of State Key Laboratory of Polymer Physics and Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences
- DOI：10.1007/s10118-015-1694-z
  中图分类号：
- 收稿：2015-04-08，
  
  修回：2015-04-28，
  
  录用：2015-04-28，
  
  纸质出版：2015-11
- Accepted：
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Zhen-bing Li, Yan-hui Xiang, Xian-jing Zhou, 等. Thermo-sensitive Poly(DEGMMA-co-MEA) Microgels: Synthesis, Characterization and Interfacial Interaction with Adsorbed Protein Layer[J]. Chinese Journal of Polymer Science, 2015,33(11):1516-1526.

Zhen-bing Li, Yan-hui Xiang, Xian-jing Zhou, et al. Thermo-sensitive Poly(DEGMMA-co-MEA) Microgels: Synthesis, Characterization and Interfacial Interaction with Adsorbed Protein Layer[J]. Chinese Journal of Polymer Science, 2015, 33(11): 1516-1526. DOI: 10.1007/s10118-015-1694-z.
Zhen-bing Li, Yan-hui Xiang, Xian-jing Zhou, 等. Thermo-sensitive Poly(DEGMMA-co-MEA) Microgels: Synthesis, Characterization and Interfacial Interaction with Adsorbed Protein Layer[J]. Chinese Journal of Polymer Science, 2015,33(11):1516-1526. DOI： 10.1007/s10118-015-1694-z.

Zhen-bing Li, Yan-hui Xiang, Xian-jing Zhou, et al. Thermo-sensitive Poly(DEGMMA-co-MEA) Microgels: Synthesis, Characterization and Interfacial Interaction with Adsorbed Protein Layer[J]. Chinese Journal of Polymer Science, 2015, 33(11): 1516-1526. DOI: 10.1007/s10118-015-1694-z. DOI：

摘要

Abstract

The novel microgels

poly[di(ethylene glycol) methyl ether methacrylate-

-2-methoxyethyl acrylate] poly(DEGMMA-

-MEA) microgels

were synthesized. The poly(DEGMMA-

-MEA) microgels were thermo-sensitive and exhibited a volume phase transitive temperature (VPTT) of 14–22 °C. The incorporation of hydrophobic comonomer MEA shifted the VPTT of poly(DEGMMA-

-MEA) microgels to lower temperatures. The interfacial interaction of poly(DEGMMA-

-MEA) microgels and three model proteins

namely fibrinogen

bovine serum albumin and lysozyme

was investigated by quartz crystal microbalance (QCM). An injection sequence of "microgel-after-protein" was then established for the real-time study of the interaction of proteins and the microgels at their swollen and collapsed states by using QCM technique. The results indicated that the interfacial interaction of poly(DEGMMA-

-MEA) microgels and adsorbed protein layers was mainly determined by the electrostatic interaction. Because poly(DEGMMA-

-MEA) microgels were negatively charged in Tris-HCl buffer solution (pH = 7.4)

the microgels did not adsorb on negatively charged fibrinogen and bovine serum albumin layers but strongly adsorbed on positively charged lysozyme layer. Stronger interaction between lysozyme and the microgels at collapsed state (

i.e.

at 37 °C) was observed. Furthermore

the incorporation of MEA might weaken the interaction between poly(DEGMMA-

-MEA) microgels and proteins.

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