Complexation between DNA and Peptides with Precisely Controlled Charge Density and Distribution

Ji-han Zhou; Hao Wen; Cui-cui Su; Lin Niu; De-hai Liang

doi:10.1007/s10118-014-1478-x

Your Location：

Home >

Browse articles >

Complexation between DNA and Peptides with Precisely Controlled Charge Density and Distribution

Updated：2021-06-21

- Complexation between DNA and Peptides with Precisely Controlled Charge Density and Distribution
- Complexation between DNA and Peptides with Precisely Controlled Charge Density and Distribution
- Chinese Journal of Polymer Science 2014年32卷第11期页码：1460-1468
- Affiliations：
  
  Beijing National Laboratory for Molecular Sciences and the Key Laboratory of Polymer Chemistry and Physics of Ministry of Education, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China
- Author bio：
  
  **De-hai Liang (梁德海), Email: dliang@pku.edu.cn
- Funds：
  
  National Natural Science Foundation of China(21174007;20990232)
- DOI：10.1007/s10118-014-1478-x
  中图分类号：
- 收稿：2014-01-03，
  
  修回：2014-02-21，
  
  录用：2014-02-25，
  
  纸质出版：2014-11
- Accepted：
Scan QR Code
Ji-han Zhou, Hao Wen, Cui-cui Su, 等. Complexation between DNA and Peptides with Precisely Controlled Charge Density and Distribution[J]. Chinese Journal of Polymer Science, 2014,32(11):1460-1468.

Ji-han Zhou, Hao Wen, Cui-cui Su, et al. Complexation between DNA and Peptides with Precisely Controlled Charge Density and Distribution[J]. Chinese Journal of Polymer Science, 2014, 32(11): 1460-1468.
Ji-han Zhou, Hao Wen, Cui-cui Su, 等. Complexation between DNA and Peptides with Precisely Controlled Charge Density and Distribution[J]. Chinese Journal of Polymer Science, 2014,32(11):1460-1468. DOI： 10.1007/s10118-014-1478-x.

Ji-han Zhou, Hao Wen, Cui-cui Su, et al. Complexation between DNA and Peptides with Precisely Controlled Charge Density and Distribution[J]. Chinese Journal of Polymer Science, 2014, 32(11): 1460-1468. DOI： 10.1007/s10118-014-1478-x.

摘要

Abstract

Using three designed peptides with precisely-controlled charge density and three types of DNAs with different length and flexibility

the effect of charge density on the formation of PEC was studied. Highly charged (KKKK)

interacts strongly with 21 bp dsDNA to form large complex

followed by precipitation; while the medium charged (KGKG)

only form complex with 21 bp dsDNA at proper +/− charge ratios; and no prominent complex between weakly charged (KGGG)5 and 21 bp dsDNA is observed at the same conditions. Similar trend is observed when the peptides form complex with 2000 bp DNA or 21nt ssDNA. It is also found that the complex formed by adding peptide to DNA is in random coil conformation

but the complex prepared by the inverse order is in molten globule state. Re-dissolution of the complex occurs only when DNA is added to peptides with similar or shorter length.

关键词

Keywords

references

浏览量

Downloads

CSCD

文章被引用时，请邮件提醒。

Submit

工具集

关联资源

A DNA Threshold of Four G/C Base Pairs Drives Intramolecular β-Sheet Formation in α-Synuclein

Polyelectrolyte Complexes and Coacervates Formed by De novo-Designed Peptides and Oligonucleotide

Core-Corona Structure Formed by Hyaluronic Acid and Poly(L-lysine) via Kinetic Path

2026-9 Contents

Two-way Shape Memory Effect of Polycaprolactone-based Polyurethane via Synergistic Role of Hydrogen Bonding and Crystallization Tuned by Malic Acid