AN AMPEROMETRIC BIOSENSOR BASED ON COVALENT IMMOBILIZATION OF ASCORBATE OXIDASE ON BIOCOMPATIABLE AND LOW-TOXIC POLY(THIOPHENE-3-ACETIC ACID) MATRIX

Dong Li; Yang-ping Wen; Jing-kun Xu; Hao-hua He; Ming Liu

doi:10.1007/s10118-012-1167-6

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AN AMPEROMETRIC BIOSENSOR BASED ON COVALENT IMMOBILIZATION OF ASCORBATE OXIDASE ON BIOCOMPATIABLE AND LOW-TOXIC POLY(THIOPHENE-3-ACETIC ACID) MATRIX

PAPER | Updated：2023-01-03

- AN AMPEROMETRIC BIOSENSOR BASED ON COVALENT IMMOBILIZATION OF ASCORBATE OXIDASE ON BIOCOMPATIABLE AND LOW-TOXIC POLY(THIOPHENE-3-ACETIC ACID) MATRIX
- AN AMPEROMETRIC BIOSENSOR BASED ON COVALENT IMMOBILIZATION OF ASCORBATE OXIDASE ON BIOCOMPATIABLE AND LOW-TOXIC POLY(THIOPHENE-3-ACETIC ACID) MATRIX
- Chinese Journal of Polymer Science 2012年30卷第5期页码：705-718
- Affiliations：
  
  a.Jiangxi Key Laboratory of Organic Chemistry, Jiangxi Science and Technology Normal University, Nanchang 330013, China
  b.Key Laboratory of Crop Physiology, Ecology and Genetic Breeding, Ministry of Education, Key Laboratory of Physiology, Ecology and Cultivation of Double Cropping Rice, Ministry of Agriculture, Jiangxi Agricultural University, Nanchang 330045, China
- Author bio：
  
  **Jing-kun Xu (徐景坤), E-mail: xujingkun@tsinghua.org.cn
  Hao-hua He (贺浩华), E-mail: hhhua64@163.com
- Funds：
  
  NSFC(50963002;51073074);Key Projects in the National Science & Technology Pillar Program in the Eleventh Five-year Plan Period(2006BAD02A04;2006BAD01A01);Jiangxi Provincial Department of Science and Technology(2006BAD01A01-2-5)
- DOI：10.1007/s10118-012-1167-6
  中图分类号：
- 收稿：2011-11-30，
  
  修回：2012-02-29，
  
  录用：2012-03-12，
  
  纸质出版：2012-09
- Accepted：
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Dong Li, Yang-ping Wen, Jing-kun Xu, 等. AN AMPEROMETRIC BIOSENSOR BASED ON COVALENT IMMOBILIZATION OF ASCORBATE OXIDASE ON BIOCOMPATIABLE AND LOW-TOXIC POLY(THIOPHENE-3-ACETIC ACID) MATRIX[J]. Chinese Journal of Polymer Science, 2012,30(5):705-718.

Dong Li, Yang-ping Wen, Jing-kun Xu, et al. AN AMPEROMETRIC BIOSENSOR BASED ON COVALENT IMMOBILIZATION OF ASCORBATE OXIDASE ON BIOCOMPATIABLE AND LOW-TOXIC POLY(THIOPHENE-3-ACETIC ACID) MATRIX[J]. Chinese Journal of Polymer Science, 2012, 30(5): 705-718.
Dong Li, Yang-ping Wen, Jing-kun Xu, 等. AN AMPEROMETRIC BIOSENSOR BASED ON COVALENT IMMOBILIZATION OF ASCORBATE OXIDASE ON BIOCOMPATIABLE AND LOW-TOXIC POLY(THIOPHENE-3-ACETIC ACID) MATRIX[J]. Chinese Journal of Polymer Science, 2012,30(5):705-718. DOI： 10.1007/s10118-012-1167-6.

Dong Li, Yang-ping Wen, Jing-kun Xu, et al. AN AMPEROMETRIC BIOSENSOR BASED ON COVALENT IMMOBILIZATION OF ASCORBATE OXIDASE ON BIOCOMPATIABLE AND LOW-TOXIC POLY(THIOPHENE-3-ACETIC ACID) MATRIX[J]. Chinese Journal of Polymer Science, 2012, 30(5): 705-718. DOI： 10.1007/s10118-012-1167-6.

摘要

Abstract

The biocompatiable and low-toxic poly(thiophene-3-acetic acid) (PTAA) matrix was successfully electrosynthesized in ionic liquid 1-butyl-3-methylimidazolium hexafluorophosphate (BmimPF

) in comparison with the electrosynthesis of PTAA matrix in acetonitrile (ACN). Ascorbate oxidase (AO) was used as a model for the development and application of biosensor. Vitamin C (VC) biosensors were facilely fabricated by the covalent immobilization of AO molecules on PTAA matrices electrosynthesized in ACN containing tetrabutylammonium tetrafluoroborate and BmimPF

respectively. Electrochemical impedance spectroscopy

scanning electron microscopy and FTIR spectroscopy indicated that AO molecules were covalently immobilized on PTAA matrices. Parameters of the as-obtained biosensors such as working potential

pH and temperature have been optimized. The amperometric biosensor based on PTAA matrix electrosynthesized in BmimPF

exhibited wider linear range

lower detection limit

higher sensitivity and bioaffinity

and better operational and storage stability than that electrosynthesized in ACN under optimal conditions. The as-obtained biosensor based on PTAA matrix electrosynthesized in BmimPF

was employed for the detection of VC content in commercial juices

and the result was close to the data given by manufacturers. Excellent results indicate that the PTAA matrix electrosynthesized in ionic liquid is a promising platform for the covalent immobilization of biologically-active species and the development of biosensors.

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