PRELIMINARY STUDY OF EXTRACTABLE PROTEIN BINDING USING MALEIC ANHYDRIDE COPOLYMER

Thirawan Nipithakul; Ladawan Watthanachote; Nanticha Kalapat

doi:10.1007/s10118-012-1112-8

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PRELIMINARY STUDY OF EXTRACTABLE PROTEIN BINDING USING MALEIC ANHYDRIDE COPOLYMER

PAPER | Updated：2023-01-03

- PRELIMINARY STUDY OF EXTRACTABLE PROTEIN BINDING USING MALEIC ANHYDRIDE COPOLYMER
- PRELIMINARY STUDY OF EXTRACTABLE PROTEIN BINDING USING MALEIC ANHYDRIDE COPOLYMER
- Chinese Journal of Polymer Science 2012年30卷第2期页码：181-189
- Affiliations：
  
  a.College of Interdisciplinary Studies, Thammasat University (Lampang), Lampang 52190, Thailand
  b.Polymer Science and Technology Programme, Faculty of Science, Mahidol University, Bangkok 10400, Thailand
- Author bio：
  
  *Thirawan Nipithakul, E-mail: thirawan@tu.ac.th
- Funds：
- DOI：10.1007/s10118-012-1112-8
  中图分类号：
- 收稿：2011-03-11，
  
  修回：2011-05-06，
  
  录用：2011-05-12，
  
  纸质出版：2012-03
- Accepted：
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Thirawan Nipithakul, Ladawan Watthanachote, Nanticha Kalapat. PRELIMINARY STUDY OF EXTRACTABLE PROTEIN BINDING USING MALEIC ANHYDRIDE COPOLYMER[J]. Chinese Journal of Polymer Science, 2012,30(2):181-189.

Thirawan Nipithakul, Ladawan Watthanachote, Nanticha Kalapat. PRELIMINARY STUDY OF EXTRACTABLE PROTEIN BINDING USING MALEIC ANHYDRIDE COPOLYMER[J]. Chinese Journal of Polymer Science, 2012, 30(2): 181-189.
Thirawan Nipithakul, Ladawan Watthanachote, Nanticha Kalapat. PRELIMINARY STUDY OF EXTRACTABLE PROTEIN BINDING USING MALEIC ANHYDRIDE COPOLYMER[J]. Chinese Journal of Polymer Science, 2012,30(2):181-189. DOI： 10.1007/s10118-012-1112-8.

Thirawan Nipithakul, Ladawan Watthanachote, Nanticha Kalapat. PRELIMINARY STUDY OF EXTRACTABLE PROTEIN BINDING USING MALEIC ANHYDRIDE COPOLYMER[J]. Chinese Journal of Polymer Science, 2012, 30(2): 181-189. DOI： 10.1007/s10118-012-1112-8.

摘要

Abstract

A preliminary study of using maleic anhydride copolymer for protein binding has been carried out. The polymeric films were prepared by compression of the purified resin and annealing the film to induce efficient back formation of the anhydride groups. The properties of the film surface were analyzed by attenuated total reflection Fourier transforms infrared spectroscopy and water contact angle measurements. The protein content was determined by Bradford assay. To obtain optimum conditions

immersion time for protein binding was examined. Results revealed that proteins can be successfully immobilized onto the film surface

via

covalent linkage. The efficiency of the covalent binding of the extractable protein to maleic anhydride-polyethylene film was estimated at 69.87 μg/cm

although the film had low anhydride content (3%) on the surface.

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