AQUEOUS SEC ANALYSIS OF CATIONIC POLYMERS AS GENE CARRIERS

Xu-lin Jiang; Yan-feng Chu; Jia Liu; Guang-yan Zhang; Ren-xi Zhuo

doi:10.1007/s10118-011-1060-8

Your Location：

Home >

Browse articles >

AQUEOUS SEC ANALYSIS OF CATIONIC POLYMERS AS GENE CARRIERS

Special Issue: The 8th International Symposium on Polyelectrolytes (ISP 2010) | Updated：2021-06-21

- AQUEOUS SEC ANALYSIS OF CATIONIC POLYMERS AS GENE CARRIERS
- AQUEOUS SEC ANALYSIS OF CATIONIC POLYMERS AS GENE CARRIERS
- Chinese Journal of Polymer Science 2011年29卷第4期页码：421-426
- Affiliations：
  
  Key Laboratory of Biomedical Polymers of Ministry of Education and Department of Chemistry, Wuhan University, Wuhan 430072, China
- Author bio：
  
  **Xu-lin Jiang (蒋序林), E-mail: xljiang@whu.edu.cn
- Funds：
  
  National Natural Science Foundation of China(20774068;21074100);Ph.D. Programs Foundation of Ministry of Education of China(20070486035)
- DOI：10.1007/s10118-011-1060-8
  中图分类号：
- 收稿：2011-04-08，
  
  修回：2011-04-29，
  
  录用：2011-05-03，
  
  纸质出版：2011
- Accepted：
Scan QR Code
Xu-lin Jiang, Yan-feng Chu, Jia Liu, 等. AQUEOUS SEC ANALYSIS OF CATIONIC POLYMERS AS GENE CARRIERS[J]. Chinese Journal of Polymer Science, 2011,29(4):421-426.

Xu-lin Jiang, Yan-feng Chu, Jia Liu, et al. AQUEOUS SEC ANALYSIS OF CATIONIC POLYMERS AS GENE CARRIERS[J]. Chinese Journal of Polymer Science, 2011, 29(4): 421-426.
Xu-lin Jiang, Yan-feng Chu, Jia Liu, 等. AQUEOUS SEC ANALYSIS OF CATIONIC POLYMERS AS GENE CARRIERS[J]. Chinese Journal of Polymer Science, 2011,29(4):421-426. DOI： 10.1007/s10118-011-1060-8.

Xu-lin Jiang, Yan-feng Chu, Jia Liu, et al. AQUEOUS SEC ANALYSIS OF CATIONIC POLYMERS AS GENE CARRIERS[J]. Chinese Journal of Polymer Science, 2011, 29(4): 421-426. DOI： 10.1007/s10118-011-1060-8.

摘要

Abstract

Cationic polymers have been receiving much attention as non-viral gene vectors. The aqueous mobile phase was optimized in combination with Shodex OHpak SB columns for size-exclusion chromatography (SEC) analysis of disulfide-containing poly(ethylene imine) (PEI) derivatives used in gene delivery. Addition of acetonitrile in mobile phase was shown to be able to suppress the hydrophobic interactions between polymer analytes and the stationary phase. The absolute molecular weights and distributions of the cationic polymers were determined directly from online SEC-MALS (multi-angle light scattering)/RI (refractive index detector). The results demonstrate that a good SEC separation of disulfide-containing PEI derivatives used in gene delivery with little band broadening was achieved.

关键词

Keywords

references

浏览量

Downloads

CSCD

文章被引用时，请邮件提醒。

Submit

工具集

关联资源

Modulating the Oxidation Degree of Linear Polyethyleneimine for Preparation of Highly Efficient and Low-cytotoxicity Degradable Gene Delivery Carriers

The Effect of NMR Setting Parameters on Molecular Weight Determination of Polyether Diols

The New Methods for Characterization of Molecular Weight of Supramolecular Polymers

Manipulating Hierarchical Aggregation Structure via Film-forming Kinetics Enables Organic Photovoltaics over 20% Efficiency

One-handed Helical Poly(m-terphenyl acetylene)s with Broad Optical Activity Range from Ultraviolet-Visible to Near-infrared Regions