Six water-soluble polysaccharide-protein complexes coded as GM1

GM2

GM3

GM4

GM5 and GM6 wereisolated from the mycelium of Ganoderma tsugae by extracting with 0.2 mol/L phosphate buffer solution at 25

40 and80℃

water at 120℃

0.5 mol/L aqueous NaOH solution at 25 and 65℃

consecutively. Their chemical components wereanalyzed by using IR

GC

HPLC and 13C-NMR

and some new results were obtained. The four samples GM1

GM2

GM3 and GM4 are heteropolysaccharide-prote in complexes

in which

α- (1→3) linked D-glucose is the major monosaccharide while galactose

mannose and ribose are the secondary ones.GM5 and GM6 are β-(1→3)-D-glucan-protein complexes.The protein content increased from 32% to 69% with the progress of isolation.Weight-average molecular mass Mw and the intrinsic viscosity [η] of the GM samples in 0.5mol/L aqueous NaCl solution at 25℃ were measured systematically by laser light scattering (LLS)

size exclusion chromatography(SEC) combined with LLS

and viscometry.The Mw of GMI to GM6 are 35.5

46.8

58.9

41.6

3.3 and 22.0×104

respectively.The conformation and molecular mass of the two fractions of sample GM5 were characterized satisfactorily by SEC-LLS without further fractionation.