APPLICATION OF NONIONIC TEMPERATURE SENSITIVE HYDROGEL FOR CONCENTRATION OF PROTEIN AQUEOUS SOLUTION*

SUN Yishi; QIU Zhiyong; HONG Yaoliang

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APPLICATION OF NONIONIC TEMPERATURE SENSITIVE HYDROGEL FOR CONCENTRATION OF PROTEIN AQUEOUS SOLUTION*

Papers | Updated：2021-06-21

- APPLICATION OF NONIONIC TEMPERATURE SENSITIVE HYDROGEL FOR CONCENTRATION OF PROTEIN AQUEOUS SOLUTION*
- APPLICATION OF NONIONIC TEMPERATURE SENSITIVE HYDROGEL FOR CONCENTRATION OF PROTEIN AQUEOUS SOLUTION*
- 高分子科学（英文版） 1992年10卷第4期页码：311-318
- Affiliations：
  
  1. Department of Chemical Engineering, Tsinghua University,Beijing
  2. Tsinghua University
  3. Beijing
- Author bio：
- Funds：
- DOI：
  中图分类号：
- 纸质出版：1992-12-20
- Accepted：
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[1]孙以实,邱志勇,洪耀良.APPLICATION OF NONIONIC TEMPERATURE SENSITIVE HYDROGEL FOR CONCENTRATION OF PROTEIN AQUEOUS SOLUTION[J].Chinese Journal of Polymer Science,1992,{4}(04):311-318.

SUN Yishi, QIU Zhiyong, HONG Yaoliang. APPLICATION OF NONIONIC TEMPERATURE SENSITIVE HYDROGEL FOR CONCENTRATION OF PROTEIN AQUEOUS SOLUTION*[J]. 1992, 10(4): 311-318.
[1]孙以实,邱志勇,洪耀良.APPLICATION OF NONIONIC TEMPERATURE SENSITIVE HYDROGEL FOR CONCENTRATION OF PROTEIN AQUEOUS SOLUTION[J].Chinese Journal of Polymer Science,1992,{4}(04):311-318. DOI：

SUN Yishi, QIU Zhiyong, HONG Yaoliang. APPLICATION OF NONIONIC TEMPERATURE SENSITIVE HYDROGEL FOR CONCENTRATION OF PROTEIN AQUEOUS SOLUTION*[J]. 1992, 10(4): 311-318. DOI：

摘要

<正> Six different N-alkyl substituted acrylarnide nonionic hydrogels were prepared and their swelling characteristics were measured. Poly N-isopropyl acrylamide （PNIPA） and poly N-n-propyl-acrylamide （PNNPA） temperature sensitive hydrogels were chosen as the nonionic temperature sensitive hydrogels for concentration of very dilute aqueous protein solution. The separation properties of PNIPA and PNNPA hydr0gels with different network dimensions were studied and the modification of the hydrogels was surveyed in order to decrease their surface adsorption of protein molecules. The experimental results of the concentration of BSA （Bovin serum albumin） dilute aqueous solution by hydroxylpropyl methacrylate （HPMA） copolymerized PNIPA hydrogel were given. The value and the limitation of concentration of dilute aqueous protein solution by this method was evaluated.

Abstract

Six different N-alkyl substituted acrylarnide nonionic hydrogels were prepared and their swelling characteristics were measured. Poly N-isopropyl acrylamide （PNIPA） and poly N-n-propyl-acrylamide （PNNPA） temperature sensitive hydrogels were chosen as the nonionic temperature sensitive hydrogels for concentration of very dilute aqueous protein solution. The separation properties of PNIPA and PNNPA hydr0gels with different network dimensions were studied and the modification of the hydrogels was surveyed in order to decrease their surface adsorption of protein molecules. The experimental results of the concentration of BSA （Bovin serum albumin） dilute aqueous solution by hydroxylpropyl methacrylate （HPMA） copolymerized PNIPA hydrogel were given. The value and the limitation of concentration of dilute aqueous protein solution by this method was evaluated.

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