IMMOBILIZATION OF GLUCOSE OXIDASE AND CELLULASE BY CHITOSAN— POLYACRYLIC ACID COMPLEX

WANG Lingzhi; JIANG Yingyan; ZHANG Changde; HUANG Dexiu

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IMMOBILIZATION OF GLUCOSE OXIDASE AND CELLULASE BY CHITOSAN— POLYACRYLIC ACID COMPLEX

Papers | Updated：2021-06-21

- IMMOBILIZATION OF GLUCOSE OXIDASE AND CELLULASE BY CHITOSAN— POLYACRYLIC ACID COMPLEX
- IMMOBILIZATION OF GLUCOSE OXIDASE AND CELLULASE BY CHITOSAN— POLYACRYLIC ACID COMPLEX
- 高分子科学（英文版） 1990年8卷第2期页码：115-120
- Affiliations：
  
  1. Institute of Chemistry, Academia Sinica, Beijing) ZHANG Changde and HUANG Dexiu
  2.   Academia Sinica
  3.   Beijing
  4.   Xi’an Jiaotong University
  5.   Xi’an
  6.   China
- Author bio：
- Funds：
- DOI：
  中图分类号：
- 纸质出版：1990-06-20
- Accepted：
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[1]王玲治,江英彦,张长德,黄德琇.IMMOBILIZATION OF GLUCOSE OXIDASE AND CELLULASE BY CHITOSAN— POLYACRYLIC ACID COMPLEX[J].Chinese Journal of Polymer Science,1990,{4}(02):115-120.

WANG Lingzhi, JIANG Yingyan, ZHANG Changde, et al. IMMOBILIZATION OF GLUCOSE OXIDASE AND CELLULASE BY CHITOSAN— POLYACRYLIC ACID COMPLEX[J]. 1990, 8(2): 115-120.
[1]王玲治,江英彦,张长德,黄德琇.IMMOBILIZATION OF GLUCOSE OXIDASE AND CELLULASE BY CHITOSAN— POLYACRYLIC ACID COMPLEX[J].Chinese Journal of Polymer Science,1990,{4}(02):115-120. DOI：

WANG Lingzhi, JIANG Yingyan, ZHANG Changde, et al. IMMOBILIZATION OF GLUCOSE OXIDASE AND CELLULASE BY CHITOSAN— POLYACRYLIC ACID COMPLEX[J]. 1990, 8(2): 115-120. DOI：

摘要

<正> This study is concerned with chitosan-polyacrylic acid complex as a carrier to immobilize glucose oxidase （GOD）and cellulase. The optimum emperature of the immobilized GOD （IG） was determined to be 60℃which is higher than that of the native GOD about 40℃. The optimum temperature of the immobilized cellulase （IC） was determined to be about 30℃higher than that of native cellulase. Both of the optimum pH of IG and IC shifted one pH unit to acid. Immobilized enzyme may be used in more wide pH range. Their storage life are much longer compared with their native states. Both of them can be reused at least 12 times.

Abstract

This study is concerned with chitosan-polyacrylic acid complex as a carrier to immobilize glucose oxidase （GOD）and cellulase. The optimum emperature of the immobilized GOD （IG） was determined to be 60℃ which is higher than that of the native GOD about 40℃. The optimum temperature of the immobilized cellulase （IC） was determined to be about 30℃ higher than that of native cellulase. Both of the optimum pH of IG and IC shifted one pH unit to acid. Immobilized enzyme may be used in more wide pH range. Their storage life are much longer compared with their native states. Both of them can be reused at least 12 times.

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