Polyelectrolyte Complexes and Coacervates Formed by <italic style="font-style: italic">De novo</italic>-Designed Peptides and Oligonucleotide

Tian-Hao Ren; De-Hai Liang

doi:10.1007/s10118-024-3096-6

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Polyelectrolyte Complexes and Coacervates Formed by De novo-Designed Peptides and Oligonucleotide

RESEARCH ARTICLE | Updated：2024-08-27

- Polyelectrolyte Complexes and Coacervates Formed by De novo-Designed Peptides and Oligonucleotide
- Chinese Journal of Polymer Science Vol. 42, Issue 9, Pages: 1333-1340(2024)
- Affiliations：
  
  Beijing National Laboratory for Molecular Sciences, Department of Polymer Science and Engineering and the Key Laboratory of Polymer Chemistry and Physics of the Ministry of Education, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China
- Author bio：
  
  dliang@pku.edu.cn
- Funds：
- DOI：10.1007/s10118-024-3096-6
  CLC：
- Published：01 September 2024，
  
  Published Online：04 March 2024，
  
  Received：27 November 2023，
  
  Revised：02 January 2024，
  
  Accepted：12 January 2024
- Accepted：
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Ren, T. H.; Liang, D. H. Polyelectrolyte complexes and coacervates formed by De novo-designed peptides and oligonucleotide. Chinese J. Polym. Sci. 2024, 42, 1333–1340

Tian-Hao Ren, De-Hai Liang. Polyelectrolyte Complexes and Coacervates Formed by De novo-Designed Peptides and Oligonucleotide. [J]. Chinese Journal of Polymer Science 42(9):1333-1340(2024)
Ren, T. H.; Liang, D. H. Polyelectrolyte complexes and coacervates formed by De novo-designed peptides and oligonucleotide. Chinese J. Polym. Sci. 2024, 42, 1333–1340 DOI： 10.1007/s10118-024-3096-6.

Tian-Hao Ren, De-Hai Liang. Polyelectrolyte Complexes and Coacervates Formed by De novo-Designed Peptides and Oligonucleotide. [J]. Chinese Journal of Polymer Science 42(9):1333-1340(2024) DOI： 10.1007/s10118-024-3096-6.

摘要

Electrostatic interaction determines the growth and size of the peptide/ss-oligo coacervates by controlling the strength of complexation and the degree of chain relaxation. The hydrophobic interaction is prominent when the charges are neutralized. The secondary structures of peptides exhibit an effect even stronger than that of the electrostatic interaction.

Abstract

The liquid-liquid phase separation of biopolymers in living cells contains multiple interactions and occurs in a dynamic environment. Resolving the regulation mechanism is still a challenge. In this work

we designed a series of peptides (XXLY)

SSSGSS and studied their complexation and coacervation behavior with single-stranded oligonucleotides. The “X” and “Y” are varied to combine known amounts of charged and non-charged amino acids

together with the introduction of secondary structures and pH responsiveness. Results show that the electrostatic interaction

which is described as charge density

controls both the strength of complexation and the degree of chain relaxation

and thus determines the growth and size of the coacervates. The hydrophobic interaction is prominent when the charges are neutralized. Interestingly

the secondary structures of peptides exhibit profound effect on the morphology of the phases

such as solid phase to liquid phase transition. Our study gains insight into the phase separation under physiological conditions. It is also helpful to create coacervates with desirable structures and functions.

关键词

Keywords

Polyelectrolyte complexCoacervatePeptidesSecondary structureElectrostatic interaction

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Polyelectrolyte Complexes and Coacervates Formed by De novo-Designed Peptides and Oligonucleotide

DOI：10.1007/s10118-024-3096-6

摘要

Abstract

关键词

Keywords

references