Thermo- and pH-sensitive Polymer with Pendant Spacer-linked Imidazole Cycles

Natalya V. Zakharova; Stanislav N. Zelinskiy; Mariya S. Strelova; Elena N. Danilovtseva; Vadim V. Annenkov

doi:10.1007/s10118-023-3056-6

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Thermo- and pH-sensitive Polymer with Pendant Spacer-linked Imidazole Cycles

RESEARCH ARTICLE | Updated：2024-03-14

- Thermo- and pH-sensitive Polymer with Pendant Spacer-linked Imidazole Cycles
  Enhanced Publication
- Chinese Journal of Polymer Science Vol. 42, Issue 4, Pages: 437-445(2024)
- Affiliations：
  
  a.Institute of Macromolecular Compounds of the Russian Academy of Sciences, Saint Petersburg 199004, Russia
  b.Limnological Institute of Siberian Branch of the Russian Academy of Sciences, Irkutsk 664033, Russia
- Author bio：
  
  Na_Zar@inbox.ru
- Funds：
- DOI：10.1007/s10118-023-3056-6
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Zakharova, N. V.; Zelinskiy, S. N.; Strelova, M. S.; Danilovtseva, E. N.; Annenkov, V. V. Thermo- and pH-sensitive polymer with pendant spacer-linked imidazole cycles. Chinese J. Polym. Sci. 2024, 42, 437–445

Natalya V. Zakharova, Stanislav N. Zelinskiy, Mariya S. Strelova, et al. Thermo- and pH-sensitive Polymer with Pendant Spacer-linked Imidazole Cycles. [J]. Chinese Journal of Polymer Science 42(4):437-445(2024)
Zakharova, N. V.; Zelinskiy, S. N.; Strelova, M. S.; Danilovtseva, E. N.; Annenkov, V. V. Thermo- and pH-sensitive polymer with pendant spacer-linked imidazole cycles. Chinese J. Polym. Sci. 2024, 42, 437–445 DOI： 10.1007/s10118-023-3056-6.

Natalya V. Zakharova, Stanislav N. Zelinskiy, Mariya S. Strelova, et al. Thermo- and pH-sensitive Polymer with Pendant Spacer-linked Imidazole Cycles. [J]. Chinese Journal of Polymer Science 42(4):437-445(2024) DOI： 10.1007/s10118-023-3056-6.

摘要

Poly(N-(3-(1H-imidazol-1-yl)propyl)acrylamide) was synthesized. The methylene units in the side chain cause the polymer thermolability at 29-48°C and in the neutral pH range. The new polymer is able to form complexes with oligo-DNA more actively than poly(1-vinylimidazole) and can be applied to create finely controlled surfaces for cell culture.

Abstract

By the reaction of poly(acryloyl chloride) with

-(3-aminopropyl)imidazole

poly(

-(3-(1

-imidazol-1-yl)propyl)acrylamide) was synthesized. The new polymer contains an imidazole ring removed from the main chain by a spacer of five bonds. The structure and purity

molecular weight

hydrodynamic and thermosensitive properties of the obtained sample were studied by

H- and

C-NMR

FTIR spectroscopy

acid-base titration

light scattering

turbidimetry and viscometry. The observed ability of the imidazole-containing polymer to form and destroy associates in water-salt solutions at pH 6.6−7.4 and temperatures of 29−48 °C indicates that these are promising candidates for designing complex biomedical systems. The new polymer is able to form complexes with oligo-DNA more actively than poly(1-vinylimidazole)

which is of interest for gene delivery applications. The polymer cross-linked with epichlorohydrin gives micro-relief coatings on the plastic surface

and the modified surface is able to attach negatively charged objects. This thermo- and pH-sensitive polymer modification can be applied to create finely controlled surfaces for cell culturing.

关键词

Keywords

Imidazole-containing polymerThermolabilitypH-sensitivityThe modified surfaceDNA immobilization

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