Regulating FUS Liquid-Liquid Phase Separation <italic style="font-style: italic">via</italic> Specific Metal Recognition

Fen Li; Yiyang Lin; Yan Qiao

doi:10.1007/s10118-022-2763-8

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Regulating FUS Liquid-Liquid Phase Separation via Specific Metal Recognition

RESEARCH ARTICLE | Updated：2022-08-19

- Regulating FUS Liquid-Liquid Phase Separation via Specific Metal Recognition
  Enhanced Publication
- Chinese Journal of Polymer Science Vol. 40, Issue 9, Pages: 1043-1049(2022)
- Affiliations：
  
  a.Beijing National Laboratory for Molecular Sciences (BNLMS), Laboratory of Polymer Physics and Chemistry, CAS Research/Education Center for Excellence in Molecular Sciences, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, China
  b.University of Chinese Academy of Sciences, Beijing 100049, China
  c.State Key Laboratory of Chemical Resource Engineering, Beijing Laboratory of Biomedical Materials, Beijing University of Chemical Technology, Beijing 100029, China
- Author bio：
  
  y.lin@mail.buct.edu.cn (Y.L.)
  yanqiao@iccas.ac.cn (Y.Q.)
- Funds：
- DOI：10.1007/s10118-022-2763-8
  CLC：
- Published：01 September 2022，
  
  Published Online：24 June 2022，
  
  Received：16 March 2022，
  
  Revised：12 April 2022，
  
  Accepted：15 April 2022
- Accepted：
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Li, F.; Lin, Y.; Qiao, Y. Regulating FUS liquid-liquid phase separation via specific metal recognition. Chinese J. Polym. Sci. 2022, 40, 1043–1049

Fen Li, Yiyang Lin, Yan Qiao. Regulating FUS Liquid-Liquid Phase Separation via Specific Metal Recognition. [J]. Chinese Journal of Polymer Science, 2022,40(9):1043-1049.
Li, F.; Lin, Y.; Qiao, Y. Regulating FUS liquid-liquid phase separation via specific metal recognition. Chinese J. Polym. Sci. 2022, 40, 1043–1049 DOI： 10.1007/s10118-022-2763-8.

Fen Li, Yiyang Lin, Yan Qiao. Regulating FUS Liquid-Liquid Phase Separation via Specific Metal Recognition. [J]. Chinese Journal of Polymer Science, 2022,40(9):1043-1049. DOI： 10.1007/s10118-022-2763-8.

摘要

We design a fused in sarcoma (FUS)-derived protein by fusing a hexhistidine-tag to the low complexity domain of FUS

which undergoes liquid-liquid phase separation in the presence of metal ions. Regulation of dynamic coacervation of FUS with the competitive binding strategy offers a possibility to design protocells as a controlled release system.

Abstract

Liquid-liquid phase separation (LLPS) or biomolecular condensation that leads to formation of membraneless organelles plays a critical role in many biochemical processes. Mechanism study of regulating LLPS is therefore central to the understanding of protein aggregation and disease-relevant process. We report a fused in sarcoma protein (FUS)-derived low complexity (LC) sequence that undergoes LLPS in the presence of metal ions. The LC protein was constructed by fusing a hexhistidine-tag to the

-terminal low complexity domain (the residues 1–165 in QGSY-rich segment) of FUS. Spontaneous condensation of the intrinsic disordered protein into coacervate droplets was observed in the presence of metal ions that chelate oligohistidine moieties to form protein matrix. We demonstrate the key role of metal ion-histidine coordination in governing LLPS behaviours and the fluidity of biomolecular condensates. By taking advantage of competitive binding using chelators

we show the possibility of regulating dynamic behaviors of disease-relevant protein droplets

and developing a potential approach towards controllable biological encapsulation/release.

关键词

Keywords

FUSLiquid-liquid phase separationBiomolecular condensateMembraneless organellesProtocellsMetal coordination

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Regulating FUS Liquid-Liquid Phase Separation via Specific Metal Recognition

DOI：10.1007/s10118-022-2763-8

摘要

Abstract

关键词

Keywords

references