A Glycosylated and Catechol-crosslinked <italic style="font-style: italic">ε</italic>-Polylysine Hydrogel: Simple Preparation and Excellent Wound Hemostasis and Healing Properties

Lin Teng; Zheng-Wei Shao; Yu-Shi He; Jia-Yu Lu; De-Rong Zou; Chuan-Liang Feng; Chang-Ming Dong

doi:10.1007/s10118-022-2741-1

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A Glycosylated and Catechol-crosslinked ε-Polylysine Hydrogel: Simple Preparation and Excellent Wound Hemostasis and Healing Properties

RESEARCH ARTICLE | Updated：2022-08-19

- A Glycosylated and Catechol-crosslinked ε-Polylysine Hydrogel: Simple Preparation and Excellent Wound Hemostasis and Healing Properties
- Chinese Journal of Polymer Science Vol. 40, Issue 9, Pages: 1110-1119(2022)
- Affiliations：
  
  a.School of Chemistry and Chemical Engineering, Frontiers Science Center for Transformative Molecules, Shanghai Key Laboratory of Electrical Insulation and Thermal Aging, Shanghai Jiao Tong University, Shanghai 200240, China
  b.Department of Stomatology, Shanghai Jiao Tong University Affiliated Sixth People’s Hospital, Shanghai Jiao Tong University, Shanghai 200233, China
  c.School of Chemistry and Chemical Engineering, Shanghai Electrochemical Energy Devices Research Center, Shanghai Jiao Tong University, Shanghai 200240, China
  d.School of Materials Science and Engineering, State Key Laboratory of Metal Matrix Composites, Shanghai Jiao Tong University, Shanghai 200240, China
- Author bio：
  
  angelinelu@sjtu.edu.cn (J.Y.L.)
  cmdong@sjtu.edu.cn (C.M.D.)
- Funds：
- DOI：10.1007/s10118-022-2741-1
  CLC：
- Published：01 September 2022，
  
  Published Online：24 June 2022，
  
  Received：10 March 2022，
  
  Revised：23 March 2022，
  
  Accepted：24 March 2022
- Accepted：
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Teng, L.; Shao, Z. W.; He, Y. S.; Lu, J. Y.; Zou, D. R.; Feng, C. L.; Dong, C. M. A glycosylated and catechol-crosslinked ε-polylysine hydrogel: simple preparation and excellent wound hemostasis and healing properties. Chinese J. Polym. Sci. 2022, 40, 1110–1119

Lin Teng, Zheng-Wei Shao, Yu-Shi He, et al. A Glycosylated and Catechol-crosslinked ε-Polylysine Hydrogel: Simple Preparation and Excellent Wound Hemostasis and Healing Properties. [J]. Chinese Journal of Polymer Science, 2022,40(9):1110-1119.
Teng, L.; Shao, Z. W.; He, Y. S.; Lu, J. Y.; Zou, D. R.; Feng, C. L.; Dong, C. M. A glycosylated and catechol-crosslinked ε-polylysine hydrogel: simple preparation and excellent wound hemostasis and healing properties. Chinese J. Polym. Sci. 2022, 40, 1110–1119 DOI： 10.1007/s10118-022-2741-1.

Lin Teng, Zheng-Wei Shao, Yu-Shi He, et al. A Glycosylated and Catechol-crosslinked ε-Polylysine Hydrogel: Simple Preparation and Excellent Wound Hemostasis and Healing Properties. [J]. Chinese Journal of Polymer Science, 2022,40(9):1110-1119. DOI： 10.1007/s10118-022-2741-1.

摘要

Inspired by bio-functions and microstructures of extracellular matrixes and mussel foot proteins

we constructed the glucose-/catechol-functionalized ε-polylysine-based hydrogels

which presented excellent hemostasis and wound healing performance

and the hydroproline level within 14 days is comparable to that in normal skins. The cooperative glycosylation and catechol-modification make commercial ε-polylysine suitable for constructing high performance wound hemostats and dressings with potential transition applications.

Abstract

Commercial tissue adhesives have been widely applied in wound hemostats and dressings while enhancing the hemostasis and healing capabilities is challenging to meet clinical needs. Herein

we designed the glucose- and catechol-functionalized derivatives from commercial

-polylysine (EPL) and prepared the hydrogels by simple amidation and catechol-crosslinking reactions

which have larger swelling ratios of 220%−240%

suitable microporous size of about 6−8 μm

and tissue adhesion strength of about 20−40 kPa. The hemolysis

cytotoxicity

and cellular double-staining assays indicate that those hydrogels had good biocompatibility and the H-3 hydrogel with higher glucose content gave a lower hemolysis ratio of 0.73%±0.14%. The blood-clotting index

blood cell attachment and adhesion studies showed those hydrogels had fast blood-coagulation

resulting in excellent hemostasis performance with a short hemostatic time of 38−46 s and less blood loss of 19%−34% in a liver hemorrhage model. A full-thickness rat-skin defect model further demonstrates that the H-3 hydrogel achieved fast wound healing with a wound closure of 70.0%±2.7% on postoperative day 7 and nearly full closure on day 14. Remarkably

the hydroproline level that denotes the collagen production reached a higher one of 7.24±0.55 μg/mg comparable to that in normal skins on day 14

evidencing the wound healing was close to completion in the H-3 treatment. Consequently

this work provides a simple method to construct a glycosylated and catechol-functionalized hydrogel platform from commercial EPL

holding translational potentials in wound hemostats and dressings.

关键词

Keywords

Polylysine hydrogelGlycosylationMussel-mimeticHemostasisWound dressing

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⁰

A Glycosylated and Catechol-crosslinked ε-Polylysine Hydrogel: Simple Preparation and Excellent Wound Hemostasis and Healing Properties

DOI：10.1007/s10118-022-2741-1

摘要

Abstract

关键词

Keywords

references