Ir(III)-based Ratiometric Hypoxic Probe for Cell Imaging

Shi-Lu Ji; Hua-Min Lan; Sen-Sen Zhou; Xiao-Ke Zhang; Wei-Zhi Chen; Xi-Qun Jiang

doi:10.1007/s10118-023-2922-6

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Ir(III)-based Ratiometric Hypoxic Probe for Cell Imaging

RESEARCH ARTICLE | Updated：2023-04-21

- Ir(III)-based Ratiometric Hypoxic Probe for Cell Imaging
- Ir(III)-based Ratiometric Hypoxic Probe for Cell Imaging
- 高分子科学（英文版） 2023年41卷第5期页码：794-801
- Affiliations：
  
  a.Department of Polymer Science & Engineering, College of Chemistry & Chemical Engineering, Nanjing University, Nanjing 210093, China
  b.College of Science, Nanjing Forestry University, Nanjing 210037, China
- Author bio：
  
  chenwz@nju.edu.cn(W.Z.C)
  jiangx@nju.edu.cn(X.Q.J)
- Funds：
  
  This work was financially supported by the National Key R&D Program of China (Nos. 2017YFA0701301 and 2017YFA0205400), the National Natural Science Foundation of China (Nos. 92163214, 51690153, 21720102005 and 51803089) and the Natural Science Foundation of Jiangsu Province (No. BK20202002).;Electronic supplementary information (ESI) is available free of charge in the online version of this article athttp://doi.org/10.1007/s10118-023-2922-6.
- DOI：10.1007/s10118-023-2922-6
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Ir(III)-based Ratiometric Hypoxic Probe for Cell Imaging[J]. 高分子科学（英文版）, 2023,41(5):794-801.

Shi-Lu Ji, Hua-Min Lan, Sen-Sen Zhou, et al. Ir(III)-based Ratiometric Hypoxic Probe for Cell Imaging[J]. Chinese Journal of Polymer Science, 2023,41(5):794-801.
Ir(III)-based Ratiometric Hypoxic Probe for Cell Imaging[J]. 高分子科学（英文版）, 2023,41(5):794-801. DOI： 10.1007/s10118-023-2922-6.

Shi-Lu Ji, Hua-Min Lan, Sen-Sen Zhou, et al. Ir(III)-based Ratiometric Hypoxic Probe for Cell Imaging[J]. Chinese Journal of Polymer Science, 2023,41(5):794-801. DOI： 10.1007/s10118-023-2922-6.

摘要

Two new ratiometric hypoxia probes (Ir-C343 and Ir-GFP) are synthesized, and a ratiometric response is realized that is only related to oxygen concentration. Ir-GFP shows promising applications in the ratiometric hypoxia imaging of cells due to its long excitation wavelength, good water solubility, high biocompatibility, and low relative fluorescence intensity compared with the phosphorescent emitter Ir-fliq.

Abstract

Two new ratiometric hypoxia probes (Ir-C343 and Ir-GFP) are synthesized by covalently incorporating florescent internal standard molecules coumarin 343 (C343) and green fluorescent protein (GFP) into bis[1-(9,9-dimethyl-9,H,-fluoren-2-yl)-isoquinoline] (succinylacetone) Ir(III) (Ir-fliq), respectively. After connecting with internal standard molecules, the Ir-fliq moiety still exhibits high sensitivity to oxygen concentration, while the fluorescence intensity of the internal standard remains relatively constant under different oxygen concentrations. As a result, a ratiometric response is realized that is only related to oxygen concentration. In addition, Ir-GFP shows more promising applications in the ratiometric hypoxia imaging of cells due to its long excitation wavelength, good water solubility, high biocompatibility, and low relative fluorescence intensity compared with the phosphorescent emitter Ir-fliq.

关键词

Keywords

Hypoxia probeRatiometric probeHypoxic responseCell imaging

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