Modulating the Oxidation Degree of Linear Polyethyleneimine for Preparation of Highly Efficient and Low-cytotoxicity Degradable Gene Delivery Carriers

Xiao-Ya Guo; Zhi-Yu Yang; Hua-Pan Fang; Dan-Hua Zhou; Xuan Pang; Hua-Yu Tian; Xue-Si Chen

doi:10.1007/s10118-024-3171-z

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Modulating the Oxidation Degree of Linear Polyethyleneimine for Preparation of Highly Efficient and Low-cytotoxicity Degradable Gene Delivery Carriers

RESEARCH ARTICLE | Updated：2024-11-04

- Modulating the Oxidation Degree of Linear Polyethyleneimine for Preparation of Highly Efficient and Low-cytotoxicity Degradable Gene Delivery Carriers
- Modulating the Oxidation Degree of Linear Polyethyleneimine for Preparation of Highly Efficient and Low-cytotoxicity Degradable Gene Delivery Carriers
- 高分子科学（英文版） 2024年42卷第11期页码：1699-1709
- Affiliations：
  
  a.Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China
  b.School of Applied Chemistry and Engineering, University of Science and Technology of China, Hefei 230026, China
  c.State Key Laboratory of Physical Chemistry of Solid Surfaces, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China
  d.Innovation Laboratory for Sciences and Technologies of Energy Materials of Fujian Province (IKKEM), Xiamen 361005, China
- Author bio：
  
  hpfang@xmu.edu.cn (H.P.F.)
  thy@xmu.edu.cn (H.Y.T.)
- Funds：
  
  This work was financially supported by National Key Research and Development Program of China (No. 2021YFB3800900), the National Natural Science Foundation of China (Nos. 51925305, 51833010 and 52203183), Natural Science Foundation of Xiamen, China (No. 3502Z202371004), Fundamental Research Funds for the Central Universities (No. 20720230004) and the talent cultivation project Funds for the Innovation Laboratory for Sciences and Technologies of Energy Materials of Fujian Province (No. HRTP-[2022]52).;Electronic supplementary information (ESI) is available free of charge in the online version of this article at http://doi.org/10.1007/s10118-024-3171-z.
- DOI：10.1007/s10118-024-3171-z
  中图分类号：
- 纸质出版日期：2024-11-30，
  
  网络出版日期：2024-08-20，
  
  收稿日期：2024-04-22，
  
  修回日期：2024-05-14，
  
  录用日期：2024-05-22
- Accepted：
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Guo, X. Y.; Yang, Z. Y.; Fang, H. P.; Zhou, D. H.; Pang, X.; Tian, H. Y.; Chen, X. S. Modulating the oxidation degree of linear polyethyleneimine for preparation of highly efficient and low-cytotoxicity degradable gene delivery carriers. Chinese J. Polym. Sci. 2024, 42, 1699–1709

Xiao-Ya Guo, Zhi-Yu Yang, Hua-Pan Fang, et al. Modulating the Oxidation Degree of Linear Polyethyleneimine for Preparation of Highly Efficient and Low-cytotoxicity Degradable Gene Delivery Carriers. [J]. Chinese Journal of Polymer Science, 2024,42(11):1699-1709.
Guo, X. Y.; Yang, Z. Y.; Fang, H. P.; Zhou, D. H.; Pang, X.; Tian, H. Y.; Chen, X. S. Modulating the oxidation degree of linear polyethyleneimine for preparation of highly efficient and low-cytotoxicity degradable gene delivery carriers. Chinese J. Polym. Sci. 2024, 42, 1699–1709 DOI： 10.1007/s10118-024-3171-z.

Xiao-Ya Guo, Zhi-Yu Yang, Hua-Pan Fang, et al. Modulating the Oxidation Degree of Linear Polyethyleneimine for Preparation of Highly Efficient and Low-cytotoxicity Degradable Gene Delivery Carriers. [J]. Chinese Journal of Polymer Science, 2024,42(11):1699-1709. DOI： 10.1007/s10118-024-3171-z.

摘要

The article describes the preparation of an efficient

degradable

simple to prepare DNA delivery carrier

oxidized PEI (oxPEI)

using the “green oxidant” hydrogen peroxide to oxidize linear PEI (LPEI). It addresses the challenge of non-degradability of PEI

which is significant for advancing the further application of PEI.

Abstract

Polyethyleneimine (PEI)

as a widely used polymer material in the field of gene delivery

has been extensively studied for modification and shielding to reduce its cytotoxicity. However

research aimed at preparing degradable PEI is scarce. In this work

the hydrogen peroxide (H

) oxidation method was used to introduce degradable amide groups in the PEI and a series of oxidized PEI22k (oxPEI22k) with different degrees of oxidation were synthesized by regulating the dosage of H

. The relationship between the oxidation degree of oxPEI22k and the gene transfection efficiency of oxPEI22k was studied in detail

confirming that the oxPE

I22k with oxidation degrees of 16.7% and 28.6% achieved improved transfection efficiency compared to unmodified PEI. These oxPEI22k also proved reduced cytotoxicity and improved degradability. Further

this strategy was extended to the synthesis of low-molecular-weight oxPEI1.8k. The oxPEI1.8k with suitable oxidation degree also achieved improved transfection efficiency and reduced cytotoxicity. In brief

this work provided high-efficiency and low-cytotoxicity degradable gene delivery carriers by regulating the oxidation degree of PEI

which was of great significance for promoting clinical applications of PEI.

关键词

Keywords

PolyethyleneimineGene deliveryGene transfectionDegradable gene carriers

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