Polyelectrolyte Complexes and Coacervates Formed by <italic style="font-style: italic">De novo</italic>-Designed Peptides and Oligonucleotide

Tian-Hao Ren; De-Hai Liang

doi:10.1007/s10118-024-3096-6

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Polyelectrolyte Complexes and Coacervates Formed by De novo-Designed Peptides and Oligonucleotide

RESEARCH ARTICLE | Updated：2024-08-27

- Polyelectrolyte Complexes and Coacervates Formed by De novo-Designed Peptides and Oligonucleotide
- Polyelectrolyte Complexes and Coacervates Formed by De novo-Designed Peptides and Oligonucleotide
- 高分子科学（英文版） 2024年42卷第9期页码：1333-1340
- Affiliations：
  
  Beijing National Laboratory for Molecular Sciences, Department of Polymer Science and Engineering and the Key Laboratory of Polymer Chemistry and Physics of the Ministry of Education, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China
- Author bio：
  
  dliang@pku.edu.cn
- Funds：
  
  This work was financially supported by the National Natural Science Foundation of China (No. 21973002). The measurements of LCSM were performed at the Analytical Instrumentation Center of Peking University. We acknowledge the assistance and support from PKUAIC (Dr. Yan Guan).;Electronic supplementary information (ESI) is available free of charge in the online version of this article at http://doi.org/10.1007/s10118-024-3096-6.
- DOI：10.1007/s10118-024-3096-6
  中图分类号：
- 纸质出版日期：2024-09-01，
  
  网络出版日期：2024-03-04，
  
  收稿日期：2023-11-27，
  
  修回日期：2024-01-02，
  
  录用日期：2024-01-12
- Accepted：
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Ren, T. H.; Liang, D. H. Polyelectrolyte complexes and coacervates formed by De novo-designed peptides and oligonucleotide. Chinese J. Polym. Sci. 2024, 42, 1333–1340

Tian-Hao Ren, De-Hai Liang. Polyelectrolyte Complexes and Coacervates Formed by De novo-Designed Peptides and Oligonucleotide[J]. Chinese Journal of Polymer Science, 2024,42(9):1333-1340.
Ren, T. H.; Liang, D. H. Polyelectrolyte complexes and coacervates formed by De novo-designed peptides and oligonucleotide. Chinese J. Polym. Sci. 2024, 42, 1333–1340 DOI： 10.1007/s10118-024-3096-6.

Tian-Hao Ren, De-Hai Liang. Polyelectrolyte Complexes and Coacervates Formed by De novo-Designed Peptides and Oligonucleotide[J]. Chinese Journal of Polymer Science, 2024,42(9):1333-1340. DOI： 10.1007/s10118-024-3096-6.

摘要

Electrostatic interaction determines the growth and size of the peptide/ss-oligo coacervates by controlling the strength of complexation and the degree of chain relaxation. The hydrophobic interaction is prominent when the charges are neutralized. The secondary structures of peptides exhibit an effect even stronger than that of the electrostatic interaction.

Abstract

The liquid-liquid phase separation of biopolymers in living cells contains multiple interactions and occurs in a dynamic environment. Resolving the regulation mechanism is still a challenge. In this work

we designed a series of peptides (XXLY)

SSSGSS and studied their complexation and coacervation behavior with single-stranded oligonucleotides. The “X” and “Y” are varied to combine known amounts of charged and non-charged amino acids

together with the introduction of secondary structures and pH responsiveness. Results show that the electrostatic interaction

which is described as charge density

controls both the strength of complexation and the degree of chain relaxation

and thus determines the growth and size of the coacervates. The hydrophobic interaction is prominent when the charges are neutralized. Interestingly

the secondary structures of peptides exhibit profound effect on the morphology of the phases

such as solid phase to liquid phase transition. Our study gains insight into the phase separation under physiological conditions. It is also helpful to create coacervates with desirable structures and functions.

关键词

Keywords

Polyelectrolyte complexCoacervatePeptidesSecondary structureElectrostatic interaction

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Polyelectrolyte Complexes and Coacervates Formed by De novo-Designed Peptides and Oligonucleotide

DOI：10.1007/s10118-024-3096-6

摘要

Abstract

关键词

Keywords

references