The Effect of Topologies and Refilling Short-chain PEG on Protein Adsorption

Chi Li; Jia-Ning Zhang; Jing Jin; Wei Jiang

doi:10.1007/s10118-023-2971-x

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The Effect of Topologies and Refilling Short-chain PEG on Protein Adsorption

RESEARCH ARTICLE | Updated：2023-11-27

- The Effect of Topologies and Refilling Short-chain PEG on Protein Adsorption
- Chinese Journal of Polymer Science Vol. 41, Issue 12, Pages: 1879-1888(2023)
- Affiliations：
  
  a.State Key Laboratory of Polymer Physics and Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China
  b.School of Applied Chemistry and Engineering, University of Science and Technology of China, Hefei 230026, China
- Author bio：
  
  jjin@ciac.ac.cn (J.J.)
  wjiang@ciac.ac.cn (W.J.)
- Funds：
- DOI：10.1007/s10118-023-2971-x
  CLC：
- Received：15 January 2023，
  
  Revised：2023-02-15，
  
  Accepted：21 February 2023，
  
  Online First：22 March 2023，
  
  Published：01 December 2023
- Accepted：
Scan QR Code
Li, C.; Zhang, J. N.; Jin, J.; Jiang, W. The effect of topologies and refilling short-chain PEG on protein adsorption. Chinese J. Polym. Sci. 2023, 41, 1879–1888

Chi Li, Jia-Ning Zhang, Jing Jin, et al. The Effect of Topologies and Refilling Short-chain PEG on Protein Adsorption[J]. Chinese Journal of Polymer Science, 2023, 41(12): 1879-1888.
Li, C.; Zhang, J. N.; Jin, J.; Jiang, W. The effect of topologies and refilling short-chain PEG on protein adsorption. Chinese J. Polym. Sci. 2023, 41, 1879–1888 DOI： 10.1007/s10118-023-2971-x.

Chi Li, Jia-Ning Zhang, Jing Jin, et al. The Effect of Topologies and Refilling Short-chain PEG on Protein Adsorption[J]. Chinese Journal of Polymer Science, 2023, 41(12): 1879-1888. DOI： 10.1007/s10118-023-2971-x.

摘要

SH-PEG and SH-PEG-SH polymers can form linear and looped conformation on the surface by gold-sulfur bonds

respectively. Refilling short-chain PEGs can be seen clearly to improve protein resistance

and the substrate conformation-dependence occurred during protein adsorption.

Abstract

PEGylation is the gold standard for constructing protein resistance surfaces. Herein

grafting mPEG-SH and SH-PEG-SH with varied molecular weights (Mw=5K

10K

and 20K) on a gold chip

and the subsequent lysozyme adsorptions of the PEG layers are evaluated using quartz-crystal microbalance based on dissipation (QCM-D). The lysozyme resistance depends on the features of grafting density and chain conformation

i.e.

linear and looped conformation. However

long-chain PEG (Mw≥10K) is insufficient to form a dense layer to resist protein due to large steric hindrances. Short-chain PEG (Mw=1K) with linear and looped structures is used to refill onto the long-chain PEG layer to increase the grafting density of PEGs and improve protein resistance. The refilling process and the subsequent protein adsorption depend on conformation rather than the density of the long-chain PEG substrate. Notably

the long-chain PEG looped substrates significantly improve protein resistance

attributing to the high viscoelasticity of the looped substrate and an increase in grafting density after refilling. Thus

refilling short-chain PEG improves protein resistance and the substrate conformation-dependence gives insight into the impact of topology

providing new ideas for how to increase chain density and select suitable topo

logy to resist protein adsorption and demonstrating a potential application in biomedical fields.

关键词

Keywords

references

Brash,J.L.;Horbett,T.A.;Latour,R.A.;Tengvall,P.Thebloodcompatibilitychallenge.Part2:proteinadsorptionphenomenagoverningbloodreactivity. Acta Biomater. 2019 , 94 ,11−24..

Bochenek,M.;Oleszko-Torbus,N.;Wałach,W.;Lipowska-Kur,D.;Dworak,A.;Utrata-Wesołek,A.Polyglycidoloflinearorbranchedarchitectureimmobilizedonasolidsupportforbiomedicalapplications. Polym. Rev. 2020 , 60 ,717−767..

Mevo,S.I.U.;Ashrafudoulla,M.;FurkanurRahamanMizan,M.;Park,S.H.;Ha,S.D.Promisingstrategiestocontrolpersistentenemies:somenewtechnologiestocombatbiofilminthefoodindustry—areview. Compr. Rev. Food Sci. Food Saf. 2021 , 20 ,5938−5964..

Ye,Z.;Zhang,P.;Zhang,J.;Deng,L.;Zhang,J.;Lin,C.;Guo,R.;Dong,A.Noveldual-functionalcoatingwithunderwaterself-healingandanti-protein-foulingpropertiesbycombiningtwokindsofmicrocapsulesandazwitterioniccopolymer. Prog. Org. Coat. 2019 , 127 ,211−221..

SabatédelRío,J.;Henry,O.Y.;Jolly,P.;Ingber,D.E.Anantifoulingcoatingthatenablesaffinity-basedelectrochemicalbiosensingincomplexbiologicalfluids. Nat. Nanotechnol. 2019 , 14 ,1143−1149..

Ahsan,S.M.;Rao,C.M.;Ahmad,M.Nanoparticle-proteininteraction:thesignificanceandroleofproteincorona. Mol. Cell. Toxicol. 2018 ,175−198..

Hedayati,M.;Marruecos,D.F.;Krapf,D.;Kaar,J.L.;Kipper,M.J.Proteinadsorptionmeasurementsonlowfoulingandultralowfoulingsurfaces:acriticalcomparisonofsurfacecharacterizationtechniques. Acta Biomater. 2020 , 102 ,169−180..

Cerchier,P.;Pezzato,L.;Gennari,C.;Moschin,E.;Moro,I.;Dabalà,M.PEOcoatingcontainingcopper:ApromisinganticorrosiveandantifoulingcoatingforseawaterapplicationofAA7075. Surf. Coat. Technol. 2020 , 393 ,125774..

Guo,L.L.;Cheng,Y.F.;Ren,X.;Gopinath,K.;Lu,Z.S.;Li,C.M.;Xu,L.Q.Simultaneousdepositionoftannicacidandpoly(ethyleneglycol)toconstructtheantifoulingpolymericcoatingontitaniumsurface. Colloids Surf. B: Biointerfaces 2021 , 200 ,111592..

Shin,E.;Lim,C.;Kang,U.J.;Kim,M.;Park,J.;Kim,D.;Choi,W.;Hong,J.;Baig,C.;Lee,D.W.;Kim,B.S.Mussel-inspiredcopolyetherloopwithsuperiorantifoulingbehavior. Macromolecules 2020 , 53 ,3551−3562..

Aghajani,M.;Esmaeili,F.Anti-biofoulingassemblystrategiesforprotein & cellrepellentsurfaces:amini-review. J. Biomater. Sci., Polym. Ed. 2021 , 32 ,1770−1789..

Zhang,K.X.;Huang,H.;Hung,H.C.;Leng,C.;Wei,S.;Crisci,R.;Jiang,S.Y.;Chen,Z.Stronghydrationatthepoly(ethyleneglycol)brush/albuminsolutioninterface. Langmuir 2020 , 36 ,2030−2036..

Hafeez,A.;Karim,Z.A.;Ismail,A.F.;Samavati,A.;Said,K.A.M.;Selambakkannu,S.Functionalizedboronnitridecompositeultrafiltrationmembranefordyeremovalfromaqueoussolution. J. Membr. Sci. 2020 , 612 ,118473..

Wang,X.;Bowman,J.;Tu,S.;Nykypanchuk,D.;Kuksenok,O.;Minko,S.PolyethyleneglycolCrowder'seffectonenzymeaggregation,thermalstability,andresidualcatalyticactivity. Langmuir 2021 , 37 ,8474−8485..

Lu,J.;Xue,Y.;Shi,R.;Kang,J.;Zhao,C.Y.;Zhang,N.N.;Wang,C.Y.;Lu,Z.Y.;Liu,K.Anon-sacrificialmethodforthequantificationofpoly(ethyleneglycol)graftingdensityongoldnanoparticlesforapplicationsinnanomedicine. Chem. Sci. 2019 , 10 ,2067−2074..

Ortiz,R.;Olsen,S.;Thormann,E.Salt-inducedcontrolofthegraftingdensityinpoly(ethyleneglycol)brushlayersbyagrafting-toapproach. Langmuir 2018 , 34 ,4455−4464..

Wang,H.;Zhang,Z.;Chen,J.;Lian,C.;Han,X.;Liu,H.Conformation-dominatedsurfaceantifoulingandaqueouslubrication. Colloids Surf. B: Biointerfaces 2022 , 214 ,112452..

Unsworth,L.;Sheardown,H.;Brash,J.Proteinresistanceofsurfacespreparedbysorptionofend-thiolatedpoly(ethyleneglycol)togold:effectofsurfacechaindensity. Langmuir 2005 , 21 ,1036−1041..

Unsworth,L.D.;Tun,Z.;Sheardown,H.;Brash,J.L. In situ neutronreflectometryinvestigationofgold-chemisorbedPEOlayersofvaryingchaindensity:relationshipoflayerstructuretoproteinresistance. J. Colloid Interface Sci. 2006 , 296 ,520−526..

Liu,Y.X.;Zhang,H.D.Structuresandsu rfacestatesofpolymerbrushesingoodsolvents:effectsofsurfaceinteractions. Chinese J. Polym. Sci. 2018 , 36 ,1047−1054..

Penna,M.;Ley,K.J.;Belessiotis-Richards,A.;MacLaughlin,S.;Winkler,D.A.;Yarovsky,I.Hydrationanddynamicsofligandsdeterminetheantifoulingcapacityoffunctionalizedsurfaces. J. Phys. Chem. C 2019 , 123 ,30360−30372..

Jeon,S.;Lee,J.;Andrade,J.;DeGennes,P.Protein—surfaceinteractionsinthepresenceofpolyethyleneoxide:I.Simplifiedtheory. J. Colloid Interface Sci. 1991 , 142 ,149−158..

Benhabbour,S.R.;Sheardown,H.;Adronov,A.ProteinresistanceofPEG-functionalizeddendronizedsurfaces:effectofPEGmolecularweightanddendrongeneration. Macromolecules 2008 , 41 ,4817−4823..

Chen,Q.;Yu,S.;Zhang,D.;Zhang,W.;Zhang,H.;Zou,J.;Mao,Z.;Yuan,Y.;Gao,C.;Liu,R.ImpactofantifoulingPEGlayerontheperformanceoffunctionalpeptidesinregulatingcellbehaviors. J. Am. Chem. Soc. 2019 , 141 ,16772−16780..

Wang,H.;Dardir,K.;Lee,K.B.;Fabris,L.Impactofproteincoronainnanoflare-basedbiomoleculardetectionandquantification. Bioconjugate Chem. 2019 , 30 ,2555−2562..

Dai,Q.;Walkey,C.;Chan,W.C.Polyethyleneglycolbackfillingmitigatesthenegativeimpactoftheproteincoronaonnanoparticlecelltargeting. Angew. Chem. Int. Ed. 2014 , 53 ,5093−5096..

Du,Y.;Jin,J.;Jiang,W.AstudyofpolyethyleneglycolbackfillingforenhancingtargetrecognitionusingQCM-DandDPI. J. Mater. Chem. B 2018 , 6 ,6217−6224..

Hu,Y.;Jin,J.;Han,Y.;Yin,J.;Jiang,W.;Liang,H.Studyoffibrinogenadsorptiononpoly(ethyleneglycol)-modifiedsurfacesusingaquartzcrystalmicrobalancewithdissipationandadualpolarizationinterferometry. RSC Adv. 2014 , 4 ,7716−7724..

Jin,J.;Han,Y.;Zhang,C.;Liu,J.;Jiang,W.;Yin,J.;Liang,H.EffectofgraftedPEGchainconformationonalbuminandlysozymeadsorption:acombinedstudyusingQCM-DandDPI. Colloids Surf., B 2015 , 136 ,838−844..

Li,B.;Yu,B.;Wang,X.L.;Guo,F.;Zhou,F.Correlationbetweenconformationchangeofpolyelectrolytebrushesandlubrication. Chinese J. Polym. Sci. 2015 , 33 ,163−172..

Han,Y.;Ma,J.;Hu,Y.;Jin,J.;Jiang,W.Effectofend-graftedpolymerconformationonproteinresistance. Langmuir 2018 , 34 ,2073−2080..

Du,Y.;Jin,J.;Liang,H.;Jiang,W.Structuralandphysicochemicalpropertiesandbiocompatibilityoflinearandloopedpolymer-cappedgoldnanoparticles. Langmuir 2019 , 35 ,8316−8324..

Lv,J.;Jin,J.;Han,Y.;Jiang,W.Effectofend-graftedPEGconformationonthehemocompatibilityofpoly(styrene- b -(ethylene- co -butylene)- b -styrene). J. Biomater. Sci., Polym. Ed. 2019 , 30 ,1670−1685..

Alexander,S.Adsorptionofchainmoleculeswithapolarheadascalingdescription. J. Phys. I 1977 , 38 ,983−987..

Jin,J.;Hu,Y.;Han,Y.;Jiang,W.Modifiedsurfacebypoly(ethyleneglycol)withloopedconformationanditssuperioranticoagulantproperty. Sci. Sin. Chim. 2018 , 48 ,972−980..

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