A Macroporous Cryogel with Enhanced Mechanical Properties for Osteochondral Regeneration <italic style="font-style: italic">In vivo</italic>

Xin-Yu Wu; Jun Yang; Fang-Hui Wu; Wang-Bei Cao; Tong Zhou; Zhao-Yi Wang; Chen-Xi Tu; Zhong-Ru Gou; Lei Zhang; Chang-You Gao

doi:10.1007/s10118-022-2835-9

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A Macroporous Cryogel with Enhanced Mechanical Properties for Osteochondral Regeneration In vivo

RESEARCH ARTICLE | Updated：2022-12-19

- A Macroporous Cryogel with Enhanced Mechanical Properties for Osteochondral Regeneration In vivo
- Chinese Journal of Polymer Science Vol. 41, Issue 1, Pages: 40-50(2023)
- Affiliations：
  
  a.Ministry of Education, Key Laboratory of Macromolecular Synthesis and Functionalization, Department of Polymer Science and Engineering, Zhejiang University, Hangzhou 310027, China
  b.Department of Orthopaedics, the Third Hospital Affiliated to Wenzhou Medical University & Rui’an People’s Hospital, Rui’an 325200, China
  c.Bio-nanomaterials and Regenerative Medicine Research Division, Zhejiang-California International Nanosystem Institute, Zhejiang University, Hangzhou 310058, China
- Author bio：
  
  cygao@zju.edu.cn
- Funds：
- DOI：10.1007/s10118-022-2835-9
  CLC：
- Received：01 June 2022，
  
  Revised：2022-6-27，
  
  Accepted：02 July 2022，
  
  Online First：08 October 2022，
  
  Published：01 January 2023
- Accepted：
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Wu, X. Y.; Yang, J.; Wu, F. H.; Cao, W. B.; Zhou, T.; Wang, Z. Y.; Tu, C. X.; Gou, Z. R.; Zhang, L.; Gao, C. Y. A macroporous cryogel with enhanced mechanical properties for osteochondral regeneration in vivo. Chinese J. Polym. Sci. 2023, 41, 40–50

Xin-Yu Wu, Jun Yang, Fang-Hui Wu, et al. A Macroporous Cryogel with Enhanced Mechanical Properties for Osteochondral Regeneration In vivo[J]. Chinese Journal of Polymer Science, 2023, 41(1): 40-50. DOI: 10.1007/s10118-022-2835-9.
Wu, X. Y.; Yang, J.; Wu, F. H.; Cao, W. B.; Zhou, T.; Wang, Z. Y.; Tu, C. X.; Gou, Z. R.; Zhang, L.; Gao, C. Y. A macroporous cryogel with enhanced mechanical properties for osteochondral regeneration in vivo. Chinese J. Polym. Sci. 2023, 41, 40–50 DOI： 10.1007/s10118-022-2835-9.

Xin-Yu Wu, Jun Yang, Fang-Hui Wu, et al. A Macroporous Cryogel with Enhanced Mechanical Properties for Osteochondral Regeneration In vivo[J]. Chinese Journal of Polymer Science, 2023, 41(1): 40-50. DOI: 10.1007/s10118-022-2835-9. DOI：

摘要

A novel cell-free hydrogel scaffold with macroporous structure and enhanced mechanical properties is designed and fabricated by using two gelatin derivatives to regenerate osteochondral defect

in situ

Abstract

The dense networks of conventional hydrogel hinder the efficient cell penetration and thus tissue regeneration. In this study

a macroporous cryogel with excellent mechanical properties was designed and fabricated through cryogelation and crosslinking of methacrylated gelatin (Gel-MA) and gelatin grafted with 2-ureido-4[1

]-6-methyl-pyrimidinone (Gel-UPY). The successful modification of the two functional units on the side chain of gelatin was confirmed by

H-NMR and infrared spectroscopy. The macroporous structure of cryogel was verified by scanning electron microscopy and confocal microscopy. The compressive modulus of cryogel (110 kPa) was 2 magnitudes higher than that of the conventional hydrogel of the same chemical compositions (8 kPa). Both the hydrogel and cryogel exhibited excellent stress-recovery ability. The cryogel could well maintain the viability of chondrocytes and facilitated their migration into the scaffold interior. After being implanted into osteochondral defects in rabbits for 12 w

the Gel-MA/Gel-UPY cryogel better facilitated the regeneration of upper cartilage and subchondral bone compared to the conventional hydrogel.

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references

Hunziker, E. B. Articular cartilage repair: basic science and clinical progress . A review of the current status and prospects . Osteoarthr. Cartil. , 2002 . 10 432 - 463 . .

Malda, J.; Groll, J.; van Weeren, P. R . Rethinking articular cartilage regeneration based on a 250-year-old statement . Nat. Rev. Rheumatol. , 2019 . 15 571 - 572 . DOI: 10.1038/s41584-019-0278-7 http://doi.org/10.1038/s41584-019-0278-7 .

Makris, E. A.; Gomoll, A. H.; Malizos, K. N.; Hu, J. C.; Athanasiou, K. A . Repair and tissue engineering techniques for articular cartilage . Nat. Rev. Rheumatol. , 2015 . 11 21 - 34 . DOI: 10.1038/nrrheum.2014.157 http://doi.org/10.1038/nrrheum.2014.157 .

Shimomura, K.; Moriguchi, Y.; Murawski, C. D.; Yoshikawa, H.; Nakamura, N . Osteochondral tissue engineering with biphasic scaffold: current strategies and techniques . Tissue Eng. Part B-Rev. , 2014 . 20 468 - 476 . DOI: 10.1089/ten.TEB.2013.0543 http://doi.org/10.1089/ten.TEB.2013.0543 .

Zhou, L.; Gjvm, V. O.; Malda, J.; Stoddart, M. J.; Lai, Y.; Richards, R. .; Ki-wai Ho, K.; Qin, L . GInnovative tissue-engineered strategies for osteochondral defect repair and regeneration: current progress and challenges . Adv. Healthc. Mater. , 2020 . 9 2001008 DOI: 10.1002/adhm.202001008 http://doi.org/10.1002/adhm.202001008 .

Xue, X.; Hu, Y.; Deng, Y.; Su, J . Recent advances in design of functional biocompatible hydrogels for bone tissue engineering . Adv. Funct. Mater. , 2021 . 31 2009432 DOI: 10.1002/adfm.202009432 http://doi.org/10.1002/adfm.202009432 .

Ngadimin, K. D.; Stokes, A.; Gentile, P.; Ferreira, A. M . Biomimetic hydrogels designed for cartilage tissue engineering . Biomater. Sci. , 2021 . 9 4246 - 4259 . DOI: 10.1039/D0BM01852J http://doi.org/10.1039/D0BM01852J .

Wu, H. Y.; Yang, L.; Tu, J. S.; Wang, J.; Li, J. G.; Lv, H. Y.; Yang, X. N . Hydrogels with dynamically controllable mechanics and biochemistry for 3D cell culture platforms . Chinese J. Polym. Sci. , 2022 . 40 38 - 46 . DOI: 10.1007/s10118-021-2639-3 http://doi.org/10.1007/s10118-021-2639-3 .

Qi, C.; Liu, J.; Jin, Y.; Xu, L.; Wang, G.; Wang, Z.; Wang, L . Photo-crosslinkable, injectable sericin hydrogel as 3D biomimetic extracellular matrix for minimally invasive repairing cartilage . Biomaterials , 2018 . 163 89 - 104 . DOI: 10.1016/j.biomaterials.2018.02.016 http://doi.org/10.1016/j.biomaterials.2018.02.016 .

Yuan, T.; Li, Z.; Zhang, Y.; Shen, K.; Zhang, X.; Xie, R.; Liu, F.; Fan, W . Injectable ultrasonication-induced silk fibroin hydrogel for cartilage repair and regeneration . Tissue Eng. Part A , 2020 . 27 17 - 18 . DOI: 10.1089/ten.TEA.2020.0323 http://doi.org/10.1089/ten.TEA.2020.0323 .

Sun, L.; Ma, Y.; Niu, H.; Liu, Y.; Yuan, Y.; Liu, C. . Recapitulation of in situ endochondral ossification using an injectable hypoxia-mimetic hydrogel . Adv. Funct. Mater. , 2020 . 31 2008515 DOI: 10.1002/adfm.202008515 http://doi.org/10.1002/adfm.202008515 .

Radhakrishnan, J.; Manigandan, A.; Chinnaswamy, P.; Subramanian, A.; Sethuraman, S . Gradient nano-engineered in situ forming composite hydrogel for osteochondral regeneration . Biomaterials , 2018 . 162 82 - 98 . DOI: 10.1016/j.biomaterials.2018.01.056 http://doi.org/10.1016/j.biomaterials.2018.01.056 .

Zhou, F.; Hong, Y.; Zhang, X.; Yang, L.; Li, J.; Jiang, D.; Bunpetch, V.; Hu, Y.; Ouyang, H.; Zhang, S . Tough hydrogel with enhanced tissue integration and in situ forming capability for osteochondral defect repair . Appl. Mater. Today , 2018 . 13 32 - 44 . DOI: 10.1016/j.apmt.2018.08.005 http://doi.org/10.1016/j.apmt.2018.08.005 .

Levato, R.; Webb, W. R.; Otto, I. A.; Mensinga, A.; Zhang, Y. D.; van Rijen, M.; van Weeren, R.; Khan, I. M.; Malda, J . The bio in the ink: cartilage regeneration with bioprintable hydrogels and articular cartilage-derived progenitor cells . Acta Biomaterialia , 2017 . 61 41 - 53 . DOI: 10.1016/j.actbio.2017.08.005 http://doi.org/10.1016/j.actbio.2017.08.005 .

Fan, C.; Wang, D. A . Macroporous hydrogel scaffolds for three-dimensional cell culture and tissue engineering . Tissue Eng. Part B-Rev. , 2017 . 23 451 - 461 . DOI: 10.1089/ten.TEB.2016.0465 http://doi.org/10.1089/ten.TEB.2016.0465 .

Hixon, K. R.; Lu, T.; Sell, S. A . A comprehensive review of cryogels and their roles in tissue engineering applications . Acta Biomaterialia , 2017 . 62 29 - 41 . DOI: 10.1016/j.actbio.2017.08.033 http://doi.org/10.1016/j.actbio.2017.08.033 .

Yuan, Z. Y.; Yuan, X. J.; Zhao, Y. M.; Cai, Q.; Wang, Y.; Luo, R. C.; Yu, S.; Wang, Y. Y.; Han, J. M.; Ge, L. H.; Huang, J. Y.; Xiong, C. Y . Injectable GelMA cryogel microspheres for modularized cell delivery and potential vascularized bone regeneration . Small , 2021 . 17 2006596 DOI: 10.1002/smll.202006596 http://doi.org/10.1002/smll.202006596 .

Yu, Y.; Li, P.; Zhu, C.; Ning, N.; Zhang, S.; Vancso, G. J . Multifunctional and recyclable photothermally responsive cryogels as efficient platforms for wound healing . Adv. Funct. Mater. , 2019 . 29 1904402 DOI: 10.1002/adfm.201904402 http://doi.org/10.1002/adfm.201904402 .

Zhang, H.; Liu, C.; Chen, L.; Dai, B . Control of ice crystal growth and its effect on porous structure of chitosan cryogels . Chem. Eng. Sci. , 2019 . 201 50 - 57 . DOI: 10.1016/j.ces.2019.02.026 http://doi.org/10.1016/j.ces.2019.02.026 .

Bencherif, S. A.; Sands, R. W.; Bhatta, D.; Arany, P.; Verbeke, C. S.; Edwards, D. A.; Mooney, D. J . Injectable preformed scaffolds with shape-memory properties . Proc. Natl. Acad. Sci. U. S. A. , 2012 . 109 19590 - 19595 . DOI: 10.1073/pnas.1211516109 http://doi.org/10.1073/pnas.1211516109 .

Ying, G.; Jiang, N.; Parra, C.; Tang, G.; Zhang, J.; Wang, H.; Chen, S.; Huang, N. P.; Xie, J.; Zhang, Y. S . Bioprinted injectable hierarchically porous gelatin methacryloyl hydrogel constructs with shape-memory properties . Adv. Funct. Mater. , 2020 . 30 2003740 DOI: 10.1002/adfm.202003740 http://doi.org/10.1002/adfm.202003740 .

Chen, T. Y.; Wen, T. K.; Dai, N. T.; Hsu, S. H . Cryogel/hydrogel biomaterials and acupuncture combined to promote diabetic skin wound healing through immunomodulation . Biomaterials , 2021 . 269 120608 DOI: 10.1016/j.biomaterials.2020.120608 http://doi.org/10.1016/j.biomaterials.2020.120608 .

Lee, S. S.; Kim, J. H.; Jeong, J.; Kim, S. H. L.; Koh, R. H.; Kim, I.; Bae, S.; Lee, H.; Hwang, N. S . Sequential growth factor releasing double cryogel system for enhanced bone regeneration . Biomaterials , 2020 . 257 120223 DOI: 10.1016/j.biomaterials.2020.120223 http://doi.org/10.1016/j.biomaterials.2020.120223 .

Han, Y.; Jia, B.; Lian, M.; Sun, B.; Wu, Q.; Sun, B.; Qiao, Z.; Dai, K . High-precision, gelatin-based, hybrid, bilayer scaffolds using melt electro-writing to repair cartilage injury . Bioact. Mater. , 2021 . 6 2173 - 2186 . DOI: 10.1016/j.bioactmat.2020.12.018 http://doi.org/10.1016/j.bioactmat.2020.12.018 .

Li, T.; Liu, B.; Jiang, Y.; Lou, Y.; Chen, K.; Zhang, D . L-polylactic acid porous microspheres enhance the mechanical properties and in vivo stability of degummed silk/silk fibroin/gelatin scaffold . Biomed. Mater. , 2021 . 16 015025 DOI: 10.1088/1748-605X/abca11 http://doi.org/10.1088/1748-605X/abca11 .

Bello, A. B.; Kim, D.; Kim, D.; Park, H.; Lee, S. H . Engineering and functionalization of gelatin biomaterials: from cell culture to medical applications . Tissue Eng. Part B-Rev. , 2020 . 26 164 - 180 . DOI: 10.1089/ten.TEB.2019.0256 http://doi.org/10.1089/ten.TEB.2019.0256 .

Ullm, S.; Krueger, A.; Tondera, C.; Gebauer, T. P.; Neffe, A. T.; Lendlein, A.; Jung, F.; Pietzsch, J . Biocompatibility and inflammatory response in vitro and in vivo to gelatin-based biomaterials with tailorable elastic properties . Biomaterials , 2014 . 35 9755 - 9766 . DOI: 10.1016/j.biomaterials.2014.08.023 http://doi.org/10.1016/j.biomaterials.2014.08.023 .

Yue, K.; Trujillo-de Santiago, G.; Alvarez, M. M.; Tamayol, A.; Annabi, N.; Khademhosseini, A . Synthesis, properties, and biomedical applications of gelatin methacryloyl (GelMA) hydrogels . Biomaterials , 2015 . 73 254 - 271 . DOI: 10.1016/j.biomaterials.2015.08.045 http://doi.org/10.1016/j.biomaterials.2015.08.045 .

Dong, Z.; Yuan, Q.; Huang, K.; Xu, W.; Liu, G.; Gu, Z . Gelatin methacryloyl (GelMA)-based biomaterials for bone regeneration . RSC Adv. , 2019 . 9 17737 - 17744 . DOI: 10.1039/C9RA02695A http://doi.org/10.1039/C9RA02695A .

Rajabi, N.; Rezaei, A.; Kharaziha, M.; Bakhsheshi-Rad, H. R.; Luo, H.; RamaKrishna, S.; Berto, F . Recent advances on bioprinted gelatin methacrylate-based hydrogels for tissue repair . Tissue Eng. Part A , 2021 . 27 679 - 702 . DOI: 10.1089/ten.TEA.2020.0350 http://doi.org/10.1089/ten.TEA.2020.0350 .

Dai, X.; Zhang, Y.; Gao, L.; Bai, T.; Wang, W.; Cui, Y.; Liu, W . A mechanically strong, highly stable, thermoplastic, and self-healable supramolecular polymer hydrogel . Adv. Mater. , 2015 . 27 3566 - 3571 . DOI: 10.1002/adma.201500534 http://doi.org/10.1002/adma.201500534 .

Zhuo, Y.; Xia, Z.; Qi, Y.; Sumigawa, T.; Wu, J.; Sestak, P.; Lu, Y.; Hakonsen, V.; Li, T.; Wang, F.; Chen, W.; Xiao, S.; Long, R.; Kitamura, T.; Li, L.; He, J.; Zhang, Z . Simultaneously toughening and stiffening elastomers with octuple hydrogen bonding . Adv. Mater. , 2021 . 33 e2008523 DOI: 10.1002/adma.202008523 http://doi.org/10.1002/adma.202008523 .

Sontjens, S. H. M.; Sijbesma, R. P.; van Genderen, M. H. P.; Meijer, E. W . Stability and lifetime of quadruply hydrogen bonded 2-ureido-4[1 H ]-pyrimidinone dimers . J. Am. Chem. Soc. , 2000 . 122 7487 - 7493 . DOI: 10.1021/ja000435m http://doi.org/10.1021/ja000435m .

Appel, W. P. J.; Portale, G.; Wisse, E.; Dankers, P. Y. W.; Meijer, E. W . Aggregation of ureido-pyrimidinone supramolecular thermoplastic elastomers into nanofibers: a kinetic analysis . Macromolecules , 2011 . 44 6776 - 6784 . DOI: 10.1021/ma201303s http://doi.org/10.1021/ma201303s .

Alavijeh, R. Z.; Shokrollahi, P.; Barzin, J . A thermally and water activated shape memory gelatin physical hydrogel, with a gel point above the physiological temperature, for biomedical applications . J. Mater. Chem. B , 2017 . 5 2302 - 2314 . DOI: 10.1039/C7TB00014F http://doi.org/10.1039/C7TB00014F .

Shi, Z.; Wang, Q.; Li, G. F.; Shou, Y. F.; Zong, H. J.; Yan, S. F.; Zhang, K. X.; Yin, J. B . Preparation and characterization of attractive poly(amino acid) hydrogels based on 2-ureido-4[1 H ]-pyrimidinone . Chinese J. Polym. Sci. , 2021 . 39 327 - 336 . DOI: 10.1007/s10118-021-2498-y http://doi.org/10.1007/s10118-021-2498-y .

Agustina Aldana, A.; Isabel Rial-Hermida, M.; Abel Abraham, G.; Concheiro, A.; Alvarez-Lorenzo, C . Temperature-sensitive biocompatible IPN hydrogels based on poly(NIPA-PEGdma) and photocrosslinkable gelatin methacrylate . Soft Mater. , 2017 . 15 341 - 349 . DOI: 10.1080/1539445x.2017.1378677 http://doi.org/10.1080/1539445x.2017.1378677 .

Folmer, B. J. B.; Sijbesma, R. P.; Versteegen, R. M.; van der Rijt, J. A. J.; Meijer, E. W . Supramolecular polymer materials: chain extension of telechelic polymers using a reactive hydrogen-bonding synthon . Adv. Mater. , 2000 . 12 874 - 878 . DOI: 10.1002/1521-4095(200006)12:12<874::AID-ADMA874>3.0.CO;2-C http://doi.org/10.1002/1521-4095(200006)12:12<874::AID-ADMA874>3.0.CO;2-C .

Onofrillo, C.; Duchi, S.; Francis, S.; O'Connell, C. D.; Caballero Aguilar, L. M.; Doyle, S.; Yue, Z.; Wallace, G. G.; Choong, P. F.; Di Bella, C . FLASH: Fluorescently labelled sensitive hydrogel to monitor bioscaffolds degradation during neocartilage generation . Biomaterials , 2021 . 264 120383 DOI: 10.1016/j.biomaterials.2020.120383 http://doi.org/10.1016/j.biomaterials.2020.120383 .

Gebauer, M.; Saas, J.; Sohler, F.; Haag, J.; Soder, S.; Pieper, M.; Bartnik, E.; Beninga, J.; Zimmer, R.; Aigner, T . Comparison of the chondrosarcoma cell line SW1353 with primary human adult articular chondrocytes with regard to their gene expression profile and reactivity to IL-1 beta . Osteoarthr. Cartil. , 2005 . 13 697 - 708 . DOI: 10.1016/j.joca.2005.04.004 http://doi.org/10.1016/j.joca.2005.04.004 .

Jarrat-Binstock, E.; Bentolila, A.; Kumar, N.; Harel, H.; Domb, A. J . Preparation, characterization, and sterilization of hydrogel sponges for iontophoretic drug-delivery use . Polym. Adv. Technol. , 2007 . 18 720 - 730 . DOI: 10.1002/pat.948 http://doi.org/10.1002/pat.948 .

Feng, X.; Xu, P.; Shen, T.; Zhang, Y.; Ye, J.; Gao, C . Influence of pore architectures of silk fibroin/collagen composite scaffolds on the regeneration of osteochondral defects in vivo . J. Mater. Chem. B , 2020 . 8 391 - 405 . DOI: 10.1039/C9TB01558B http://doi.org/10.1039/C9TB01558B .

Petersen, A.; Princ, A.; Korus, G.; Ellinghaus, A.; Leemhuis, H.; Herrera, A.; Klaumunzer, A.; Schreivogel, S.; Woloszyk, A.; Schmidt-Bleek, K.; Geissler, S.; Heschel, I.; Duda, G. N . A biomaterial with a channel-like pore architecture induces endochondral healing of bone defects . Nat. Commun. , 2018 . 9 4430 DOI: 10.1038/s41467-018-06504-7 http://doi.org/10.1038/s41467-018-06504-7 .

Zhang, Q.; Lu, H. X.; Kawazoe, N.; Chen, G. P . Pore size effect of collagen scaffolds on cartilage regeneration . Acta Biomaterialia , 2014 . 10 2005 - 2013 . DOI: 10.1016/j.actbio.2013.12.042 http://doi.org/10.1016/j.actbio.2013.12.042 .

Di Luca, A.; Lorenzo-Moldero, I.; Mota, C.; Lepedda, A.; Auhl, D.; Van Blitterswijk, C.; Moroni, L . Tuning cell differentiation into a 3D scaffold presenting a pore shape gradient for osteochondral regeneration . Adv. Healthc. Mater. , 2016 . 5 1753 - 1763 . DOI: 10.1002/adhm.201670074 http://doi.org/10.1002/adhm.201670074 .

Di Luca, A.; Ostrowska, B.; Lorenzo-Moldero, I.; Lepedda, A.; Swieszkowski, W.; Van Blitterswijk, C.; Moroni, L . Gradients in pore size enhance the osteogenic differentiation of human mesenchymal stromal cells in three-dimensional scaffolds . Sci. Rep. , 2016 . 6 22898 DOI: 10.1038/srep22898 http://doi.org/10.1038/srep22898 .

Darling, E. M.; Athanasiou, K. A . Biomechanical strategies for articular cartilage regeneration . Ann. Biomed. Eng. , 2003 . 31 1114 - 24 . DOI: 10.1114/1.1603752 http://doi.org/10.1114/1.1603752 .

Armiento, A. R.; Stoddart, M. J.; Alini, M.; Eglin, D . Biomaterials for articular cartilage tissue engineering: learning from biology . Acta Biomater , 2018 . 65 1 - 20 . DOI: 10.1016/j.actbio.2017.11.021 http://doi.org/10.1016/j.actbio.2017.11.021 .

Bhosale, A. M.; Richardson, J. B . Articular cartilage: structure, injuries and review of management . British Medical Bulletin , 2008 . 87 77 - 95 . DOI: 10.1093/bmb/ldn025 http://doi.org/10.1093/bmb/ldn025 .

Fu, Q.; Rahaman, M. N.; Bal, B. S.; Bonewald, L. F.; Kuroki, K.; Brown, R. F . Silicate, borosilicate, and borate bioactive glass scaffolds with controllable degradation rate for bone tissue engineering applications. II. In vitro and in vivo biological evaluation . J. Biomed. Mater. Res. Part A , 2010 . 95A 172 - 179 . .

Sung, H. J.; Meredith, C.; Johnson, C.; Galis, Z. S . The effect of scaffold degradation rate on three-dimensional cell growth and angiogenesis . Biomaterials , 2004 . 25 5735 - 5742 . DOI: 10.1016/j.biomaterials.2004.01.066 http://doi.org/10.1016/j.biomaterials.2004.01.066 .

Yang, F.; Wang, J.; Cao, L.; Chen, R.; Tang, L.; Liu, C . Injectable and redox-responsive hydrogel with adaptive degradation rate for bone regeneration . J. Mater. Chem. B , 2014 . 2 295 - 304 . DOI: 10.1039/C3TB21103G http://doi.org/10.1039/C3TB21103G .

Zhao, X.; Xu, H.; Ye, G.; Li, C.; Wang, L.; Hu, F.; Qiu, X . Temperature-activated PRP-cryogel for long-term osteogenesis of adipose-derived stem cells to promote bone repair . Mater. Chem. Front. , 2021 . 5 396 - 405 . DOI: 10.1039/D0QM00493F http://doi.org/10.1039/D0QM00493F .

Chopra, V.; Thomas, J.; Sharma, A.; Panwar, V.; Kaushik, S.; Ghosh, D . A bioinspired, ice-templated multifunctional 3D cryogel composite crosslinked through in situ reduction of GO displayed improved mechanical, osteogenic and antimicrobial properties . Mater. Sci. Eng. C-Mater. Biol. Appl. , 2021 . 119 111584 DOI: 10.1016/j.msec.2020.111584 http://doi.org/10.1016/j.msec.2020.111584 .

Gu, L.; Zhang, J.; Li, L.; Du, Z.; Gai, Q.; Yang, X . Hydroxyapatite nanowire cornposited gelatin cryogel with improved mechanical properties and cell migration for bone regeneration . Biomed. Mater. , 2019 . 14 045001 DOI: 10.1088/1748-605X/ab1583 http://doi.org/10.1088/1748-605X/ab1583 .

Liao, H. T.; Shalumon, K. T.; Chang, K. H.; Sheu, C.; Chen, J. P . Investigation of synergistic effects of inductive and conductive factors in gelatin-based cryogels for bone tissue engineering . J. Mater. Chem. B , 2016 . 4 1827 - 1841 . DOI: 10.1039/C5TB02496J http://doi.org/10.1039/C5TB02496J .

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A Macroporous Cryogel with Enhanced Mechanical Properties for Osteochondral Regeneration In vivo

DOI：10.1007/s10118-022-2835-9

摘要

Abstract

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Keywords

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