Thermoresponsive Nanogels from Dendronized Copolymers for Complexation, Protection and Release of Nucleic Acids

Yi Yao; Jian-Hui Wu; Shi-Jie Cao; Bi-Yi Xu; Jia-Tao Yan; Di Wu; Wen Li; Afang Zhang

doi:10.1007/s10118-020-2452-4

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Thermoresponsive Nanogels from Dendronized Copolymers for Complexation, Protection and Release of Nucleic Acids

ARTICLE | Updated：2020-07-07

- Thermoresponsive Nanogels from Dendronized Copolymers for Complexation, Protection and Release of Nucleic Acids
- Chinese Journal of Polymer Science Vol. 38, Issue 11, Pages: 1164-1170(2020)
- Affiliations：
  
  Department of Polymer Materials, School of Materials Science & Engineering, Shanghai University, Shanghai 200444, China
- Author bio：
  
  wli@shu.edu.cn (W.L.)
  azhang@shu.edu.cn (A.Z.)
- Funds：
- DOI：10.1007/s10118-020-2452-4
  CLC：
- Received：03 March 2020，
  
  Revised：2020-4-4，
  
  Accepted：06 May 2020，
  
  Online First：07 July 2020，
  
  Published：14 August 2020
- Accepted：
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Yao, Y.; Wu, J. H.; Cao, S. J.; Xu, B. Y.; Yan, J. T.; Wu, D.; Li, W.; Zhang, A. Thermoresponsive nanogels from dendronized copolymers for complexation, protection and release of nucleic acids. Chinese J. Polym. Sci. 2020, 38, 1164–1170

Yi Yao, Jian-Hui Wu, Shi-Jie Cao, et al. Thermoresponsive Nanogels from Dendronized Copolymers for Complexation, Protection and Release of Nucleic Acids[J]. Chinese Journal of Polymer Science, 2020, 38(11): 1164-1170.
Yao, Y.; Wu, J. H.; Cao, S. J.; Xu, B. Y.; Yan, J. T.; Wu, D.; Li, W.; Zhang, A. Thermoresponsive nanogels from dendronized copolymers for complexation, protection and release of nucleic acids. Chinese J. Polym. Sci. 2020, 38, 1164–1170 DOI： 10.1007/s10118-020-2452-4.

Yi Yao, Jian-Hui Wu, Shi-Jie Cao, et al. Thermoresponsive Nanogels from Dendronized Copolymers for Complexation, Protection and Release of Nucleic Acids[J]. Chinese Journal of Polymer Science, 2020, 38(11): 1164-1170. DOI： 10.1007/s10118-020-2452-4.

摘要

Abstract

A series of thermoresponsive cationic dendronized copolymers and their corresponding nanogels containing dendritic oligoethylene glycol (OEG) units and guanidine groups were prepared

and their complexation

protection

and release of nucleic acids were investigated. The dendritic OEGs endow these copolymer materials with good biocompatibility and characteristic thermoresponsiveness

while cationic guanidine groups can efficiently bind with the nucleic acids. The dendritic topology also affords the copolymers specific shielding effect which plays an essential role in protecting the activity of nucleic acids. At room temperature

dendronized copolymers and the corresponding nanogels could efficiently capture and condense the nucleic acids

while above their cloud points (

more than 75% of siRNA could be released in 1 h triggered by ATP. More importantly

the copolymer showed protective capability to siRNA

while nanogels exhibit even better protection when compared to the copolymers due to the synergetic effect from the three-dimensional cross-linked network and high density of dendritic units in vicinity. This kind of smart dendronized copolymer nanogels form a novel class of scaffolds as promising materials for biomedical applications.

关键词

Keywords

references

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