Effects of Branching Strategy on the Gene Transfection of Highly Branched Poly(<italic style="font-style: italic">β</italic>-amino ester)s

Yao Wang; Chen-Fei Wang; Ming Li; De-Zhong Zhou; Wei Huang; Wen-Xin Wang

doi:10.1007/s10118-020-2393-y

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Effects of Branching Strategy on the Gene Transfection of Highly Branched Poly(β-amino ester)s

ARTICLE | Updated：2020-04-17

- Effects of Branching Strategy on the Gene Transfection of Highly Branched Poly(β-amino ester)s
- Chinese Journal of Polymer Science Vol. 38, Issue 8, Pages: 830-839(2020)
- Affiliations：
  
  a.School of Chemistry and Chemical Engineering, State Key Laboratory of Metal Matrix Composites, Shanghai Jiao Tong University, Shanghai 200240, China
  b.School of Chemical Engineering and Technology, Xi'an Jiaotong University, Xi'an 710049, China
  c.Charles Institute of Dermatology, School of Medicine, University College Dublin, Dublin 4, Ireland
  d.School of Mechanical and Materials Engineering, University College Dublin, Dublin 4, Ireland
  e.Department of Dermatology, Xinhua Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China
- Author bio：
  
  dezhong.zhou@xjtu.edu.cn (D.Z.Z.)
  wenxin.wang@ucd.ie (W.X.W.)
- Funds：
- DOI：10.1007/s10118-020-2393-y
  CLC：
- Received：21 December 2019，
  
  Revised：2020-1-12，
  
  Accepted：17 January 2020，
  
  Online First：17 April 2020，
  
  Published：2020-08
- Accepted：
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Wang, Y.; Wang, C. F.; Li, M.; Zhou, D. Z.; Huang W.; Wang, W. X. Effects of branching strategy on the gene transfection of highly branched poly(, β,-amino ester)s. Chinese J. Polym. Sci. 2020, 38, 830–839

Yao Wang, Chen-Fei Wang, Ming Li, et al. Effects of Branching Strategy on the Gene Transfection of Highly Branched Poly(β-amino ester)s[J]. Chinese Journal of Polymer Science, 2020, 38(8): 830-839. DOI: 10.1007/s10118-020-2393-y.
Wang, Y.; Wang, C. F.; Li, M.; Zhou, D. Z.; Huang W.; Wang, W. X. Effects of branching strategy on the gene transfection of highly branched poly(, β,-amino ester)s. Chinese J. Polym. Sci. 2020, 38, 830–839 DOI： 10.1007/s10118-020-2393-y.

Yao Wang, Chen-Fei Wang, Ming Li, et al. Effects of Branching Strategy on the Gene Transfection of Highly Branched Poly(β-amino ester)s[J]. Chinese Journal of Polymer Science, 2020, 38(8): 830-839. DOI: 10.1007/s10118-020-2393-y. DOI：

摘要

Abstract

Highly branched poly(

-amino ester)s (HPAEs) have emerged as one type of the most viable non-viral gene delivery vectors

both

in vitro

and

in vivo

. However

the effects of different branching strategies on the gene transfection performance have not yet been explored. Here

using triacrylate (B3) and diamine (B4) as the branching monomers

a series of HPAEs were synthesized

via

the “A2 + B3 + C2” and “A2 + B4 + C2” strategies

respectively. Results show that the branching strategy plays a pivotal role in dictating the physiological properties of the HPAE/DNA polyplexes and thus leads to obviously different cell viability and transfection efficiency. Comparatively

HPAEs synthesized

via

the “A2 + B3 + C2” branching strategy are more favorable for DNA transfection than that synthesized

via

the “A2 + B4 + C2” strategy. This study may provide new insights into the development of HPAEs based non-viral DNA delivery system.

关键词

Keywords

references

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⁰

Effects of Branching Strategy on the Gene Transfection of Highly Branched Poly(β-amino ester)s

DOI：10.1007/s10118-020-2393-y

摘要

Abstract

关键词

Keywords

references