Mediating the Migration of Mesenchymal Stem Cells by Dynamically Changing the Density of Cell-selective Peptides Immobilized on <italic style="font-style: italic">β</italic>-Cyclodextrin-modified Cell-resisting Polymer Brushes

Wang Du; De-Teng Zhang; Xue-Mei Wang; Tan-Chen Ren; Chang-You Gao

doi:10.1007/s10118-019-2324-y

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Mediating the Migration of Mesenchymal Stem Cells by Dynamically Changing the Density of Cell-selective Peptides Immobilized on β-Cyclodextrin-modified Cell-resisting Polymer Brushes

ARTICLE | Updated：2019-09-27

- Mediating the Migration of Mesenchymal Stem Cells by Dynamically Changing the Density of Cell-selective Peptides Immobilized on β-Cyclodextrin-modified Cell-resisting Polymer Brushes
- Chinese Journal of Polymer Science Vol. 38, Issue 2, Pages: 126-136(2020)
- Affiliations：
  
  Ministry of Education Key Laboratory of Macromolecular Synthesis and Functionalization, Department of Polymer Science and Engineering, Zhejiang University, Hangzhou 310027, China
- Author bio：
  
  cygao@zju.edu.cn
- Funds：
- DOI：10.1007/s10118-019-2324-y
  CLC：
- Received：26 May 2019，
  
  Revised：2019-6-15，
  
  Accepted：26 June 2019，
  
  Online First：27 September 2019，
  
  Published：2020-02
- Accepted：
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Du, W.; Zhang, D. T.; Wang, X. M.; Ren, T. C.; Gao, C. Y. Mediating the migration of mesenchymal stem cells by dynamically changing the density of cell-selective peptides immobilized on , β,-cyclodextrin-modified cell-resisting polymer brushes. Chinese J. Polym. Sci. 2020, 38, 126–136.

Wang Du, De-Teng Zhang, Xue-Mei Wang, et al. Mediating the Migration of Mesenchymal Stem Cells by Dynamically Changing the Density of Cell-selective Peptides Immobilized on β-Cyclodextrin-modified Cell-resisting Polymer Brushes[J]. Chinese Journal of Polymer Science, 2020, 38(2): 126-136. DOI: 10.1007/s10118-019-2324-y.
Du, W.; Zhang, D. T.; Wang, X. M.; Ren, T. C.; Gao, C. Y. Mediating the migration of mesenchymal stem cells by dynamically changing the density of cell-selective peptides immobilized on , β,-cyclodextrin-modified cell-resisting polymer brushes. Chinese J. Polym. Sci. 2020, 38, 126–136. DOI： 10.1007/s10118-019-2324-y.

Wang Du, De-Teng Zhang, Xue-Mei Wang, et al. Mediating the Migration of Mesenchymal Stem Cells by Dynamically Changing the Density of Cell-selective Peptides Immobilized on β-Cyclodextrin-modified Cell-resisting Polymer Brushes[J]. Chinese Journal of Polymer Science, 2020, 38(2): 126-136. DOI: 10.1007/s10118-019-2324-y. DOI：

摘要

Abstract

Dynamic control of mesenchymal stem cell (MSC) behaviors on biomaterial surface is critically involved in regulating the cell fate and tissue regeneration. Herein

a stimuli-responsive surface based on host-guest interaction with cell selectivity was developed to regulate migration of MSCs

in situ

by dynamic display of cell-specific peptides. Azobenzene-grafted MSC-affinitive peptides (EPLQLKM

Azo-E7) were grafted to

-cyclodextran (

-CD)-modified poly(2-hydroxyethyl methacrylate)-

-poly(2-hydroxyethyl methacrylate-

-glycidyl methacrylate) (PHG) brushes

which were prepared by using surface-initiated atom transfer radical polymerization (SI-ATRP). X-ray photoelectron spectroscopy (XPS)

quartz crystal microbalance (QCM)

and water contact angle were used to characterize their structure and property. Cell adhesion assay showed that the combination effect of resisting property of PHG and MSC-affinity of E7 could promote the selective adhesion of MSCs over other types of cells such as RAW264.7 macrophages and NIH3T3 fibroblasts to some extent. UV-Vis spectroscopy proved that the competing guest molecules

amantadine hydrochloride (Ama)

could release Azo-E7 peptides from the CD surface to different extents

and the effect was enhanced when UV irradiation was employed simultaneously. As a result

the decrease of cell adhesion density and migration rate could be achieved

in situ

. The cell density and migration rate could be reduced by over 40% by adding 20 μmol/L Ama

suggesting that this type of surface is a new platform for dynamic regulation of stem cell behaviors

in situ

关键词

Keywords

references

Ermis, M.; Antmen, E.; Hasirci, V . Micro and nanofabrication methods to control cell-substrate interactions and cell behavior: A review from the tissue engineering perspective . Bioact. Mater. , 2018 . 3 355 - 369 . DOI: 10.1016/j.bioactmat.2018.05.005 http://doi.org/10.1016/j.bioactmat.2018.05.005 .

Byron, A.; Morgan, M. R.; Humphries, M. J . Adhesion signalling complexes . Curr. Biol. , 2010 . 20 R1063 - R1067 . DOI: 10.1016/j.cub.2010.10.059 http://doi.org/10.1016/j.cub.2010.10.059 .

Geiger, B.; Spatz, J. P.; Bershadsky, A. D . Environmental sensing through focal adhesions . Nat. Rev. Microbiol. , 2009 . 10 21 - 33 . DOI: 10.1038/nrm2593 http://doi.org/10.1038/nrm2593 .

Zamani, M.; Prabhakaran, M. P.; Thian, E. S.; Ramakrishna, S . Controlled delivery of stromal derived factor-1 α from poly(lactic- co -glycolic acid) core-shell particles to recruit mesenchymal stem cells for cardiac regeneration . J. Colloid Interface Sci. , 2015 . 451 144 - 152 . DOI: 10.1016/j.jcis.2015.04.005 http://doi.org/10.1016/j.jcis.2015.04.005 .

Yan, C.; Sun, J.; Ding, J. D . Critical areas of cell adhesion on micropatterned surfaces . Biomaterials , 2011 . 32 3931 - 3938 . DOI: 10.1016/j.biomaterials.2011.01.078 http://doi.org/10.1016/j.biomaterials.2011.01.078 .

Okuyama, H.; Krishnamachary, B.; Zhou, Y. F.; Nagasawa, H.; Bosch-Marce, M.; Semenza, G. L . Expression of vascular endothelial growth factor receptor 1 in bone marrow-derived mesenchymal cells is dependent on hypoxia-inducible factor 1 . J. Biol. Chem. , 2006 . 281 15554 - 15563 . DOI: 10.1074/jbc.M602003200 http://doi.org/10.1074/jbc.M602003200 .

Wu, C. T.; Chang, J.; Zhai, W. Y.; Ni, S. Y.; Wang, J. Y . Porous akermanite scaffolds for bone tissue engineering: preparation, characterization, and in vitro studies . J. Biomed. Mater. Res. B , 2006 . 78B 47 - 55 . DOI: 10.1002/(ISSN)1552-4981 http://doi.org/10.1002/(ISSN)1552-4981 .

Charras, G.; Sahai, E . Physical influences of the extracellular environment on cell migration . Nat. Rev. Microbiol. , 2014 . 15 813 - 824 . DOI: 10.1038/nrm3897 http://doi.org/10.1038/nrm3897 .

Chan, C. E.; Odde, D. J . Traction dynamics of filopodia on compliant substrates . Science , 2008 . 322 1687 - 1691 . DOI: 10.1126/science.1163595 http://doi.org/10.1126/science.1163595 .

Shabbir, S. H.; Eisenberg, J. L.; Mrksich, M . An inhibitor of a cell adhesion receptor stimulates cell Migration . Angew. Chem. Int. Ed. , 2010 . 49 7706 - 7709 . DOI: 10.1002/anie.v49:42 http://doi.org/10.1002/anie.v49:42 .

Wu, J. D.; Mao, Z. W.; Gao, C. Y . Controlling the migration behaviors of vascular smooth muscle cells by methoxy poly(ethylene glycol) brushes of different molecular weight and density . Biomaterials , 2012 . 33 810 - 820 . DOI: 10.1016/j.biomaterials.2011.10.022 http://doi.org/10.1016/j.biomaterials.2011.10.022 .

Biazar, E.; Khorasani, M. T.; Joupari, M. D . Cell adhesion and surface properties of polystyrene surfaces grafted with poly( N -isopropylacrylamide) . Chinese J. Polym Sci. , 2013 . 31 1509 - 1518 . DOI: 10.1007/s10118-013-1335-3 http://doi.org/10.1007/s10118-013-1335-3 .

Cao, Z. Q.; Bian, Q.; Chen, Y.; Liang, F. X.; Wang, G. J . Light-responsive Janus-particle-based coatings for cell capture and release . ACS Macro Lett. , 2017 . 6 1124 - 1128 . DOI: 10.1021/acsmacrolett.7b00714 http://doi.org/10.1021/acsmacrolett.7b00714 .

Chen, Y. H.; Chang, S. H.; Wang, T. J.; Wang, I.; Young, T. H . Cell fractionation on pH-responsive chitosan surface . Biomaterials , 2013 . 34 854 - 863 . DOI: 10.1016/j.biomaterials.2012.10.014 http://doi.org/10.1016/j.biomaterials.2012.10.014 .

Xiao, Y.; Zhou, H. Y.; Xuan, N. X.; Cheng, M.; Rao, Y. F.; Luo, Y.; Wang, B.; Tang, R . Effective and selective cell retention and recovery from whole blood by electroactive thin films . ACS Appl. Mater. Interfaces , 2014 . 6 20804 - 20811 . DOI: 10.1021/am505072z http://doi.org/10.1021/am505072z .

Liu, H. Y.; Korc, M.; Lin, C. C . Biomimetic and enzyme-responsive dynamic hydrogels for studying cell-matrix interactions in pancreatic ductal adenocarcinoma . Biomaterials , 2018 . 160 24 - 36 . DOI: 10.1016/j.biomaterials.2018.01.012 http://doi.org/10.1016/j.biomaterials.2018.01.012 .

Costa, P.; Gautrot, J. E.; Connelly, J. T . Directing cell migration using micropatterned and dynamically adhesive polymer brushes . Acta Biomater. , 2014 . 10 2415 - 2422 . DOI: 10.1016/j.actbio.2014.01.029 http://doi.org/10.1016/j.actbio.2014.01.029 .

Boekhoven, J.; Perez, C. M. R.; Sur, S.; Worthy, A.; Stupp, S. I . Dynamic display of bioactivity through host-guest chemistry . Angew. Chem. Int. Ed. , 2013 . 52 12077 - 12080 . DOI: 10.1002/anie.201306278 http://doi.org/10.1002/anie.201306278 .

Voskuhl, J.; Sankaran, S.; Jonkheijm, P . Optical control over bioactive ligands at supramolecular surfaces . Chem. Commun. , 2014 . 50 15144 - 15147 . DOI: 10.1039/C4CC03184A http://doi.org/10.1039/C4CC03184A .

Gong, Y. H.; Li, C.; Yang, J.; Wang, H. Y.; Zhuo, R. X.; Zhang, X. Z . Photoresponsive "smart template" via host-guest interaction for reversible cell adhesion . Macromolecules , 2011 . 44 7499 - 7502 . DOI: 10.1021/ma201676w http://doi.org/10.1021/ma201676w .

Deng, J.; Liu, X. Y.; Shi, W. B.; Cheng, C.; He, C.; Zhao, C. S . Light-triggered switching of reversible and alterable biofunctionality via β -cyclodextrin/azobenzene-based host-guest interaction . ACS Macro Lett. , 2014 . 3 1130 - 1133 . DOI: 10.1021/mz500568k http://doi.org/10.1021/mz500568k .

Bian, Q.; Wang, W.; Wang, S.; Wang, G . Light-triggered specific cancer cell release from cyclodextrin/azobenzene and aptamer-modified substrate . ACS Appl. Mater. Interfaces , 2016 . 8 27360 - 27367 . DOI: 10.1021/acsami.6b09734 http://doi.org/10.1021/acsami.6b09734 .

Gu, L. Q.; Braha, O.; Conlan, S.; Cheley, S.; Bayley, H . Stochastic sensing of organic analytes by a pore-forming protein containing a molecular adapter . Nature , 1999 . 398 686 - 690 . DOI: 10.1038/19491 http://doi.org/10.1038/19491 .

Cheng, M. J.; Shi, F.; Li, J. S.; Lin, Z. F.; Jiang, C.; Xiao, M.; Zhang, L. Q.; Yang, W. T.; Nishi, T . Macroscopic supramolecular assembly of rigid building blocks through a flexible spacing coating . Adv. Mater. , 2014 . 26 3009 - 3013 . DOI: 10.1002/adma.201305177 http://doi.org/10.1002/adma.201305177 .

Khan, M.; Yang, J.; Shi, C. C.; Lv, J.; Feng, Y. K.; Zhang, W. C . Surface tailoring for selective endothelialization and platelet inhibition via a combination of SI-ATRP and click chemistry using Cys-Ala-Gly-peptide . Acta Biomater. , 2015 . 20 69 - 81 . DOI: 10.1016/j.actbio.2015.03.032 http://doi.org/10.1016/j.actbio.2015.03.032 .

Ren, T. C.; Yu, S.; Mao, Z. W.; Gao, C. Y . A complementary density gradient of zwitterionic polymer brushes and NCAM peptides for selectively controlling directional migration of schwann cells . Biomaterials , 2015 . 56 58 - 67 . DOI: 10.1016/j.biomaterials.2015.03.052 http://doi.org/10.1016/j.biomaterials.2015.03.052 .

Ji, Y.; Wei, Y.; Liu, X. S.; Wang, J. L.; Ren, K. F.; Ji, J . Zwitterionic polycarboxybetaine coating functionalized with REDV peptide to improve selectivity for endothelial cells . J. Biomed. Mater. Res. A , 2012 . 100A 1387 - 1397 . DOI: 10.1002/jbm.a.v100a.6 http://doi.org/10.1002/jbm.a.v100a.6 .

Zheng, X. W.; Pan, X.; Pang, Q.; Shuai, C. A.; Ma, L.; Gao, C. Y . Selective capture of mesenchymal stem cells over fibroblasts and immune cells on E7-modified collagen substrates under flow circumstances . J. Mater. Chem. B , 2018 . 6 165 - 173 . DOI: 10.1039/C7TB02812A http://doi.org/10.1039/C7TB02812A .

Man, Z. T.; Yin, L.; Shao, Z. X.; Zhang, X.; Hu, X. Q.; Zhu, J. X.; Dai, L. H.; Huang, H. J.; Yuan, L.; Zhou, C. Y . The effects of co-delivery of BMSC-affinity peptide and rhTGF- β 1 from coaxial electrospun scaffolds on chondrogenic differentiation . Biomaterials , 2014 . 35 5250 - 5260 . DOI: 10.1016/j.biomaterials.2014.03.031 http://doi.org/10.1016/j.biomaterials.2014.03.031 .

Shao, Z. X.; Zhang, X.; Pi, Y. B.; Wang, X. K.; Jia, Z. Q.; Zhu, J. X.; Dai, L. H.; Chen, W. Q.; Yin, L.; Chen, H. F . Polycaprolactone electrospun mesh conjugated with an MSC affinity peptide for MSC homing in vivo . Biomaterials , 2012 . 33 3375 - 3387 . DOI: 10.1016/j.biomaterials.2012.01.033 http://doi.org/10.1016/j.biomaterials.2012.01.033 .

Yang, M.; Chu, L. Y.; Wang, H. D.; Xie, R.; Song, H.; Niu, C. H . A thermoresponsive membrane for chiral resolution . Adv. Funct. Mater. , 2008 . 18 652 - 663 . DOI: 10.1002/(ISSN)1616-3028 http://doi.org/10.1002/(ISSN)1616-3028 .

Tugulu, S.; Arnold, A.; Sielaff, I.; Johnsson, K.; Klok, H. A . Protein-functionalized polymer brushes . Biomacromolecules , 2005 . 6 1602 - 1607 . DOI: 10.1021/bm050016n http://doi.org/10.1021/bm050016n .

Ren, T. C.; Mao, Z. W.; Guo, J.; Gao, C. Y . Directional migration of vascular smooth muscle cells guided by a molecule weight gradient of poly(2-hydroxyethyl methacrylate) brushes . Langmuir , 2013 . 29 6386 - 6395 . DOI: 10.1021/la4004609 http://doi.org/10.1021/la4004609 .

Ren, T. C.; Mao, Z. W.; Moya, S. E.; Gao, C. Y . Immobilization of enzymes on 2-hydroxyethyl methacrylate and glycidyl methacrylate copolymer brushes . Chem. Asian J. , 2014 . 9 2132 - 2139 . DOI: 10.1002/asia.v9.8 http://doi.org/10.1002/asia.v9.8 .

Huang, S.; Xu, L. L.; Sun, Y. X.; Wu, T. Y.; Wang, K. X.; Li, G . An improved protocol for isolation and culture of mesenchymal stem cells from mouse bone marrow . J. Orthop. Transl. , 2015 . 3 26 - 33 . DOI: 10.1016/j.jot.2014.07.005 http://doi.org/10.1016/j.jot.2014.07.005 .

Li, X.; Wang, M. M.; Wang, L.; Shi, X. J.; Xu, Y. J.; Song, B.; Chen, H . Block copolymer modified surfaces for conjugation of biomacromolecules with control of quantity and activity . Langmuir , 2013 . 29 1122 - 1128 . DOI: 10.1021/la3044472 http://doi.org/10.1021/la3044472 .

Yan, S.; Wang, Z. N.; Gao, X. L.; Gao, C. J . Antifouling PVDF ultrafiltration membranes incorporating PVDF- g -PHEMA additive via atom transfer radical graft polymerizations . J. Membr. Sci. , 2012 . 413-414 38 - 47 . DOI: 10.1016/j.memsci.2012.03.055 http://doi.org/10.1016/j.memsci.2012.03.055 .

Zhu, L. J.; Zhu, L. P.; Jiang, J. H.; Yi, Z.; Zhao, Y. F.; Zhu, B. K.; Xu, Y. Y . Hydrophilic and anti-fouling polyethersulfone ultrafiltration membranes with poly(2-hydroxyethyl methacrylate) grafted silica nanoparticles as additive . J. Membr. Sci. , 2014 . 451 157 - 168 . DOI: 10.1016/j.memsci.2013.09.053 http://doi.org/10.1016/j.memsci.2013.09.053 .

Barbey, R.; Klok, H . Room temperature, aqueous post-polymerization modification of glycidyl methacrylate-containing polymer brushes prepared via surface-initiated atom transfer radical polymerization . Langmuir , 2010 . 26 18219 - 18230 . DOI: 10.1021/la102400z http://doi.org/10.1021/la102400z .

Kim, M.; Lee, B.; Yang, K.; Park, J.; Jeon, S.; Um, S. H.; Kim, D.; Im, S. G.; Cho, S . BMP-2 peptide-functionalized nanopatterned substrates for enhanced osteogenic differentiation of human mesenchymal stem cells . Biomaterials , 2013 . 34 7236 - 7246 . DOI: 10.1016/j.biomaterials.2013.06.019 http://doi.org/10.1016/j.biomaterials.2013.06.019 .

Zhou, W. F.; Yang, M.; Zhao, Z.; Li, S. P.; Cheng, Z. Q.; Zhu, J. S . Controlled hierarchical architecture in poly[oligo(ethylene glycol) methacrylate- b -glycidyl methacrylate] brushes for enhanced label-free biosensing . Appl. Surf. Sci. , 2018 . 450 236 - 243 . DOI: 10.1016/j.apsusc.2018.04.183 http://doi.org/10.1016/j.apsusc.2018.04.183 .

Hu, W. H.; Liu, Y. S.; Lu, Z. S.; Li, C. M . Poly[oligo(ethylene glycol) methacrylate- co -glycidyl methacrylate] brush substrate for sensitive surface plasmon resonance imaging protein arrays . Adv. Funct. Mater. , 2010 . 20 3497 - 3503 . DOI: 10.1002/adfm.v20:20 http://doi.org/10.1002/adfm.v20:20 .

Lei, Z.; Gao, J. X.; Liu, X.; Liu, D. J.; Wang, Z. X . Poly(glycidyl methacrylate- co -2-hydroxyethyl methacrylate) brushes as peptide/protein microarray substrate for improving protein binding and functionality . ACS Appl. Mater. Interfaces , 2016 . 8 10174 - 10182 . DOI: 10.1021/acsami.6b01156 http://doi.org/10.1021/acsami.6b01156 .

Wu, S.; Du, W.; Duan, Y. Y.; Zhang, D. T.; Liu, Y. X.; Wu, B. B.; Zou, X. H.; Ouyang, H. W.; Gao, C. Y . Regulating the migration of smooth muscle cells by a vertically distributed poly(2-hydroxyethyl methacrylate) gradient on polymer brushes covalently immobilized with RGD peptides . Acta Biomater. , 2018 . 75 75 - 92 . DOI: 10.1016/j.actbio.2018.05.046 http://doi.org/10.1016/j.actbio.2018.05.046 .

Dehghani, E. S.; Spencer, N. D.; Ramakrishna, S. N.; Benetti, E. M . Crosslinking polymer brushes with ethylene glycol-containing segments: influence on physicochemical and antifouling properties . Langmuir , 2016 . 32 10317 - 10327 . DOI: 10.1021/acs.langmuir.6b02958 http://doi.org/10.1021/acs.langmuir.6b02958 .

Mandal, J.; Varunprasaath, R. S.; Yan, W.; Divandari, M.; Spencer, N. D.; Dubner, M . In situ monitoring of SI-ATRP throughout multiple reinitiations under flow by means of a quartz crystal microbalance . RSC Adv. , 2018 . 8 20048 - 20055 . DOI: 10.1039/C8RA03073A http://doi.org/10.1039/C8RA03073A .

Fuhrmann, I.; Karger-Kocsis, J . Photoinitiated grafting of glycidyl methacrylate and methacrylic acid on ground tire rubber . J. Appl. Polym. Sci. , 2003 . 89 1622 - 1630 . DOI: 10.1002/(ISSN)1097-4628 http://doi.org/10.1002/(ISSN)1097-4628 .

Tabata, Y . Biomaterial technology for tissue engineering applications . J. R. Soc. Interface , 2009 . 63 S311 - S324 . DOI: 10.1098/rsif.2008.0448.focus http://doi.org/10.1098/rsif.2008.0448.focus .

Ren, T. C.; Ni, Y. L.; Du, W.; Yu, S.; Mao, Z.; Gao, C. Y . Dual responsive surfaces based on host-guest interaction for dynamic mediation of cell-substrate interaction and cell migration . Adv. Mater. Interfaces , 2017 . 4 1500865 DOI: 10.1002/admi.201500865 http://doi.org/10.1002/admi.201500865 .

Lauffenburger, D. A.; Horwitz, A. F . Cell migration: a physically integrated molecular process . Cell , 1996 . 84 359 - 369 . DOI: 10.1016/S0092-8674(00)81280-5 http://doi.org/10.1016/S0092-8674(00)81280-5 .

Dimilla, P. A.; Stone, J. A.; Quinn, J. A.; Albelda, S. M.; Lauffenburger, D. A . Maximal migration of human smooth muscle cells on fibronectin and type IV collagen occurs at an intermediate attachment strength . J. Cell Bio. , 1993 . 122 729 - 737 . DOI: 10.1083/jcb.122.3.729 http://doi.org/10.1083/jcb.122.3.729 .

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Mediating the Migration of Mesenchymal Stem Cells by Dynamically Changing the Density of Cell-selective Peptides Immobilized on β-Cyclodextrin-modified Cell-resisting Polymer Brushes

DOI：10.1007/s10118-019-2324-y

摘要

Abstract

关键词

Keywords

references