Degradable Protein-loaded Polymer Capsules Fabricated by Thiol-disulfide Cross-linking Reaction at Liquid-liquid Interface

Xiaoteng Ma; Guangda Han; Hanying Zhao

doi:10.1007/s10118-019-2253-9

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Degradable Protein-loaded Polymer Capsules Fabricated by Thiol-disulfide Cross-linking Reaction at Liquid-liquid Interface

ARTICLE | Updated：2021-02-18

- Degradable Protein-loaded Polymer Capsules Fabricated by Thiol-disulfide Cross-linking Reaction at Liquid-liquid Interface
- Chinese Journal of Polymer Science Vol. 37, Issue 8, Pages: 790-796(2019)
- Affiliations：
  
  Key Laboratory of Functional Polymer Materials, Ministry of Education, College of Chemistry, Nankai University, Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), Tianjin 300071, China
- Author bio：
  
  hyzhao@nankai.edu.cn
- Funds：
- DOI：10.1007/s10118-019-2253-9
  CLC：
- Received：28 January 2019，
  
  Revised：2019-3-3，
  
  Accepted：11 March 2019，
  
  Online First：12 April 2019，
  
  Published：2019-08
- Accepted：
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Citation: Ma, X.; Han, G.; Zhao, H. Degradable protein-loaded polymer capsules fabricated by thiol-disulfide cross-linking reaction at liquid-liquid interface. Chinese J. Polym. Sci. 2019, 37, 790–796

Xiaoteng Ma, Guangda Han, Hanying Zhao. Degradable Protein-loaded Polymer Capsules Fabricated by Thiol-disulfide Cross-linking Reaction at Liquid-liquid Interface[J]. Chinese Journal of Polymer Science, 2019, 37(8): 790-796.
Citation: Ma, X.; Han, G.; Zhao, H. Degradable protein-loaded polymer capsules fabricated by thiol-disulfide cross-linking reaction at liquid-liquid interface. Chinese J. Polym. Sci. 2019, 37, 790–796 DOI： 10.1007/s10118-019-2253-9.

Xiaoteng Ma, Guangda Han, Hanying Zhao. Degradable Protein-loaded Polymer Capsules Fabricated by Thiol-disulfide Cross-linking Reaction at Liquid-liquid Interface[J]. Chinese Journal of Polymer Science, 2019, 37(8): 790-796. DOI： 10.1007/s10118-019-2253-9.

摘要

Abstract

In these years

the encapsulation of proteins for protection and delivery purpose has attracted great interest. In this research

W/O emulsion droplets were used as soft templates and bovine serum albumin (BSA) encapsulated hollow capsules were prepared by liquid-liquid interfacial thiol-disulfide exchange reaction. Block copolymer chains with pendant pyridyl disulfide groups are located at liquid-liquid interface

and upon addition of a macromolecular crosslinking agent with multiple pendant thiol groups into an emulsion

thiol-disulfide interfacial crosslinking reactions lead to the formation of BSA encapsulated hollow capsules. The cleavage of disulfides on the membranes results in the degradation of hollow structures and the release of encapsulated protein molecules. Transmission electron microscopy

scanning electron microscopy

atomic force microscopy

and confocal laser scanning microscopy were employed to characterize the hollow capsules. In comparison with native BSA

BSA molecules encapsulated in the hollow structures show higher catalytic efficiency due to higher local concentration of reactants in the structures. The membranes of the hollow capsules can efficiently protect the encapsulated BSA from hydrolysis by trypsin.

关键词

Keywords

references

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